RESUMEN
Ruthenium(II) complexes containing phenazine ring have attracted attention to design as 'molecular light switches'. In this study, we synthesized two ruthenium complexes containing phenazine ring and studied their abilities to function as DNA intercalators, DNA 'light switches', DNA topo I inhibitors, DNA photocleavers and potential antitumor reagents. [Ru(bpy)2(mbipz)](PF6)2 (1) (bpy = 2,2'-bipyridine, mbipz = 2-(4'-methyl-bipyridine-4-yl)-1H-imidazo[4,5-b]phenazine) exhibited off-on type DNA 'light swtich' behavior. DNA binding modes of the two complexes were determined as intercalation by using UV-vis spectra, emission spectra, viscosity and molecular docking experiments. DNA photocleavage experimental results showed that the two ruthenium complexes effectively cleave plasmid DNA by producing singlet oxygen. Furthermore, they displayed good topo I inhibition activities. We further found that the two complexes displayed good antitumor activities against Eca-109 cells and A549 cells. The results demonstrated that introduction of phenazine ring will be helpful to design DNA 'light switch' based on ruthenium complex.Communicated by Ramaswamy H. Sarma.
Asunto(s)
Rutenio , ADN , ADN-Topoisomerasas de Tipo I , Simulación del Acoplamiento Molecular , FenazinasRESUMEN
A new strategy for a G-quadruplex fluorescent probe based on a nitro-substituted ruthenium complex is described. G-quadruplex DNA can be distinguished from double- or single-strand DNA by the naked eye. This ability originates from variation of the degree of protection of the nitro group on the complex from water by G-quadruplex and other structure DNAs.