RESUMEN
Financial elements and R&D elements are significant drivers for enhancing regional innovation efficiency. This paper measures financial elements by financial development scale and the marketization level of the financial industry and R&D elements by the inputs and flow of R&D personnel and R&D capital and specifically considers R&D element flow to quantify the consequential spatial spillover effects. Based on provincial panel data from 2008 to 2018, the paper firstly estimates the regional innovation efficiency of China's 30 provincial-level administrative regions using super-efficiency DEA and then conducts an empirical analysis of the influence of financial elements and R&D elements on regional innovation efficiency by the use of the Tobit model and three spatial econometric models. It is found that, by and large, the financial development scale, the marketization level of the financial industry, the inputs of R&D personnel and R&D capital, and R&D capital flow all have significant effects on regional innovation efficiency. Nonetheless, by region, R&D personnel flow in central China can significantly boost regional innovation efficiency while fails in eastern and western China. From the spatial perspective, both financial elements and R&D elements have significant positive spatial spillover effects. Therefore, in order to bolster regional innovation efficiency, it is crucial to improve the allocation of financial elements and R&D elements and build a tight regional collaborative innovation network.
Asunto(s)
Desarrollo Económico , Industrias , Modelos Econométricos , Eficiencia , ChinaRESUMEN
Sulforaphane (SFN) is an isothiocyanate (ITC) derived from a glucosinolate, glucoraphinin found in cruciferous vegetables. There are few studies that focus on the role of SFN in angiogenesis under hypoxic conditions. The effect of SFN on angiogenesis and the underlying mechanisms including the roles of Nrf2 and mitochondrial dynamics were investigated using cultured human umbilical vein endothelial cells (HUVECs) in hypoxia. SFN at low doses (1.25-5 µM) increased hypoxia-induced HUVEC migration and tube formation, and alleviated hypoxia-induced retarded proliferation, but high doses (≥10 µM) exhibited an opposite effect. Under hypoxia, the expression of Nrf2 and heme oxygenase-1 was up-regulated by SFN treatment. Nrf2 knockdown abrogated SFN (2.5 µM)-induced tube formation and further potentiated the inhibitory effect of SFN (10 µM) on angiogenesis. Meanwhile, the mitochondrial function, morphology and expression of dynamic-related proteins suggested that low-dose SFN protected against hypoxia-induced mitochondrial injury and alleviated hypoxia-induced fission Nrf2-dependently without affecting the expression of key effector proteins (Drp1, Fis1, Mfn1/2 and Opa1), while high concentrations (≥10 µM SFN) aggravated hypoxia-induced mitochondrial injury, fission and Drp1 expression, and inhibited Mfn1/2 expression. These findings suggest that SFN biphasically affected the angiogenic capacity of hypoxia challenged HUVECs potentially via mechanisms involving an integrated modulation of Nrf2 and mitochondrial dynamics.
Asunto(s)
Alimentos Funcionales , Isotiocianatos/farmacología , Sulfóxidos/farmacología , Verduras , Apoptosis/efectos de los fármacos , Células Endoteliales de la Vena Umbilical Humana/efectos de los fármacos , Humanos , Hipoxia , Isotiocianatos/administración & dosificación , Isotiocianatos/química , Dinámicas Mitocondriales/efectos de los fármacos , Factor 2 Relacionado con NF-E2/metabolismo , Sulfóxidos/administración & dosificación , Sulfóxidos/químicaRESUMEN
In this work, the anti-Escherichia coli activity of the bioactive substances produced by Bacillus amyloliquefaciens R3 was examined. A new and cheap medium for production of the anti-E. coli substances which contained 20.0 g L(-1) soybean powder, 20.0 g L(-1) wheat flour, pH 6.0 was developed. A crude surfactant concentration of 0.48 mg mL(-1) was obtained after 27 h of 10-L fermentation, and the diameter of the clear zone on the plate seeded with the pathogenic E. coli 2# was 23.3 mm. A preliminary characterization suggested that the anti-E. coli substances produced by B. amyloliquefaciens R3 were the biosurfactins (F1, F2, F3, F4, and F5) with amino acids (GLLVDLL) and hydroxy fatty acids (of 12-15 carbons in length). It was found that all the strains of the pathogenic E. coli showed resistance to several different antibiotics, suggesting that they were the multi-drug resistance and all the strains of the pathogenic E. coli were sensitive to the biosurfactins, indicating that the biosurfactins produced by B. amyloliquefaciens R3 had a broad spectrum of antibacterial activity against the pathogenic E. coli with multi-drug resistant profiles. After the treatment with the purified biosurfactin (F1), the cell membrane of both the whole cells and protoplasts of the E. coli 2# was damaged and the whole cells of the bacterium were broken.