RESUMEN
OBJECTIVES: Mood stabilizers influence the morphology, chemotaxis, and survival of neurons, which are considered to be related to the mood-stabilizing effects of these drugs. Although previous studies suggest glial abnormalities in patients with bipolar disorder and an effect of mood stabilizers on certain genes in astrocytes, less is known about the effects of mood stabilizers in astrocytes than in neurons. The present study identifies a common underlying response to mood stabilizers in astrocytes. METHODS: Human astrocyte-derived cells (U-87 MG) were treated with the four most commonly used mood stabilizers (lithium, valproic acid, carbamazepine, and lamotrigine) and subjected to microarray gene expression analyses. The most prominently regulated genes were validated by qRT-PCR and western blot analysis. The intercellular localization of one of these regulated genes, fasciculation and elongation protein zeta 1 (FEZ1), was evaluated by immunofluorescence staining. RESULTS: The microarray data indicated that FEZ1 was the only gene commonly induced by the four mood stabilizers in human astrocyte-derived cells. An independent experiment confirmed astrocytic FEZ1 induction at both the transcript and protein levels following mood stabilizer treatments. FEZ1 localized to the cytoplasm of transformed and primary astrocytes from the human adult brain. CONCLUSIONS: Our data suggest that FEZ1 may play important roles in human astrocytes, and that mood stabilizers might exert their cytoprotective and mood-stabilizing effects by inducing FEZ1 expression in astrocytes.
Asunto(s)
Antimaníacos/farmacología , Astrocitos/efectos de los fármacos , Regulación de la Expresión Génica/efectos de los fármacos , Factores de Transcripción/metabolismo , Línea Celular Transformada , Análisis por Conglomerados , Perfilación de la Expresión Génica/métodos , Humanos , Péptidos y Proteínas de Señalización Intracelular/genética , Péptidos y Proteínas de Señalización Intracelular/metabolismo , Proteínas del Tejido Nervioso/genética , Proteínas del Tejido Nervioso/metabolismo , Neuronas/efectos de los fármacos , Análisis de Secuencia por Matrices de Oligonucleótidos/métodos , Transporte de Proteínas/efectos de los fármacos , Factores de Transcripción/genéticaRESUMEN
The radionuclide pair (214)Bi and (214)Po which belongs to the uranium series interferes with airborne radionuclide measurement needed for the radiation management of a nuclear facility. Time intervals between (214)Bi (ß) and (214)Po (α) are much shorter than artificial radionuclides due to the short half-life of (214)Po (164 µs). The purpose of this study is to develop of a new analytical method (time interval analysis: TIA) based on the beta-alpha coincidence method for selective measurement of (214)Bi-(214)Po. The developed method was applied to an actual dust-filter measurement. The TIA system was highly effective in measuring of the filter with background subtraction.