RESUMEN
OBJECTIVE: To study the effects of hemostasis, analgesic and anti inflammation of the alcohol extract of Hibiscus tiliaceus and offer pharmacological and experimental basis for its safe and effective use in clinic. METHODS: The effects of hemostasist were observed with tail breaking method, capillary tube method and slide method; Hot board and body distortion induced by acetic acid methods were applied in mice analgesia experiment, the mice model of acute auricle swelling induced by dmi ethylbenzene and capillary permeability induced by acetic acid were applied to observe the anti inflammatory effects. RESULTS: The alcohol extract of Hibiscus tiliaceus could significantly reduce the bleeding time and the clotting time, delay the plant reaction time and reduce the writhing times of the mice, and it also had effect on inhibiting swelling of mice ear and the permeability of the capillary. CONCLUSION: These results suggest that the alcohol extract of Hibiscus tiliaceus has the effects of hemostasis, analgesic and anti inflammation.
Asunto(s)
Analgésicos/farmacología , Antiinflamatorios no Esteroideos/farmacología , Hemostáticos/farmacología , Hibiscus/química , Extractos Vegetales/farmacología , Analgésicos/administración & dosificación , Analgésicos/aislamiento & purificación , Animales , Antiinflamatorios no Esteroideos/administración & dosificación , Antiinflamatorios no Esteroideos/aislamiento & purificación , Conducta Animal/efectos de los fármacos , Permeabilidad Capilar/efectos de los fármacos , Modelos Animales de Enfermedad , Edema/inducido químicamente , Edema/tratamiento farmacológico , Etanol , Hemostáticos/administración & dosificación , Hemostáticos/aislamiento & purificación , Ratones , Ratones Endogámicos , Dolor/inducido químicamente , Dolor/tratamiento farmacológico , Dimensión del Dolor , Extractos Vegetales/administración & dosificación , Extractos Vegetales/aislamiento & purificación , Hojas de la Planta/química , Tallos de la Planta/química , Distribución Aleatoria , Xilenos/efectos adversosRESUMEN
OBJECTIVE: To develop an HPLC method for content determination of protocatechuic acid, chlorogenic acid and caffeic acid in Zhuang medicine Blumea megacephala (Randeria), and explore the content variation of the 3 components of the herbs harvesting in different months, and provide the scientific basis of reasonable application. METHOD: The determination was carried out on a Shimadzu VP-ODS column (4.6 mm x 250 mm, 5 microm) eluted with acetonitrile and water containing 0.1% phosphoric acid in a linear gradient elution. The flow rate was 1 mL min(-1), and the detected wavelength was set at 258, 327nm. RESULT: The peak areas and the concentrations of the three components had good linear relationship in the range of 1.7-17 mg x L(-1) for protocatechuic acid, 15.6-156 mg x L(-1) for chlorogenic acid, 3.96-39.6 mg x L(-1) for caffeic acid. The average recoveries were 103.4%, 102.2%, 98.5%, respectively. CONCLUSION: The method was proved to be simple, accurate and used for the quality evaluation of Blumea megacephala (Randeria).