RESUMEN

Klebsiella oxytoca causes several diseases in immunocompromised as well as healthy individuals. Increasing resistance to a number of antibiotics makes treatment options limited. Prevention using vaccine could be an important solution to get rid of infections caused by Klebsiella oxytoca. In recent time, genome based approaches have contributed significantly in vaccine development. Our aim was to identify the most conserved and immunogenic antigens that can be considered as potential vaccine candidates. KEGG database was used to find out pathways unique to the bacteria. Subcellular localization of the protein sequences taken from the selected 36 pathways were predicted using PSORTb v3.0.2 and CELLO v2.5. Prediction of B cell epitope and the probability of the antigenicity were evaluated by using IEDB and Vaxijen respectively. BLASTp was done to find out the similarity of the selected proteins with the human proteome. Proteins failing to comply with the set parameters were filtered at each step. Finally, we identified 6 surface exposed proteins as potential vaccine candidates against Klebsiella oxytoca.

Asunto(s)

Vacunas Bacterianas/inmunología , Simulación por Computador , Klebsiella oxytoca/inmunología , Vacunas Bacterianas/química , Humanos

RESUMEN

In the past few decades, genome-based approaches have contributed significantly to vaccine development. Our aim was to identify the most conserved and immunogenic antigens of Streptococcus pneumoniae, which can be potential vaccine candidates in the future. BLASTn was done to identify the most conserved antigens. PSORTb 3.0.2 was run to predict the subcellular localization of the proteins. B cell epitope prediction was done for the immunogenicity testing. Finally, BLASTp was done for verifying the extent of similarity to human proteome to exclude the possibility of autoimmunity. Proteins failing to comply with the set parameters were filtered at each step. Based on the above criteria, out of the initial 22 pneumococcal proteins selected for screening, pavB and pullulanase were the most promising candidate proteins.

Asunto(s)

Antígenos Bacterianos/química , Proteínas Bacterianas/química , Genoma Bacteriano/inmunología , Glicósido Hidrolasas/química , Streptococcus pneumoniae/genética , Factores de Virulencia/química , Secuencia de Aminoácidos , Antígenos Bacterianos/genética , Antígenos Bacterianos/inmunología , Linfocitos B/inmunología , Linfocitos B/microbiología , Proteínas Bacterianas/genética , Proteínas Bacterianas/inmunología , Biología Computacional , Secuencia Conservada , Epítopos de Linfocito B/química , Epítopos de Linfocito B/genética , Epítopos de Linfocito B/inmunología , Glicósido Hidrolasas/genética , Glicósido Hidrolasas/inmunología , Humanos , Datos de Secuencia Molecular , Infecciones Neumocócicas/inmunología , Infecciones Neumocócicas/microbiología , Infecciones Neumocócicas/prevención & control , Vacunas Neumococicas/genética , Vacunas Neumococicas/inmunología , Proteoma/genética , Proteoma/inmunología , Streptococcus pneumoniae/inmunología , Factores de Virulencia/genética , Factores de Virulencia/inmunología