RESUMEN

The cytochrome P450 enzyme-encoding genes rosC and rosD were cloned from the rosamicin biosynthetic gene cluster of Micromonospora rosaria IFO13697. The functions of RosC and RosD were demonstrated by gene disruption and complementation with M. rosaria and bioconversion of rosamicin biosynthetic intermediates with Escherichia coli expressing RosC and RosD. It is proposed that M. rosaria IFO13697 has two pathway branches that lead from the first desosaminyl rosamicin intermediate, 20-deoxo-20-dihydro-12,13-deepoxyrosamicin, to rosamicin.

Asunto(s)

Antibacterianos/biosíntesis , Proteínas Bacterianas/metabolismo , Sistema Enzimático del Citocromo P-450/metabolismo , Leucomicinas/biosíntesis , Micromonospora/enzimología , Micromonospora/genética , Proteínas Bacterianas/genética , Sistema Enzimático del Citocromo P-450/genética , Escherichia coli/enzimología , Escherichia coli/genética , Eliminación de Gen , Prueba de Complementación Genética , Familia de Multigenes