RESUMEN
The number of antral follicles is considered an important fertility trait because animals with a high follicle count (HFC) produce more oocytes and embryos per cycle. Identification of these animals by genetic markers such as single nucleotide polymorphisms (SNPs) can accelerate selection of future generations. The aim of this study was to perform a genome wide association study (GWAS) on Nelore and Angus heifers with HFC and low (LFC) antral follicle counts. The groups HFC and LFC for genotyping were formed based on the average of total follicles (≥ 3 mm) counted in each breed consistently ± standard deviation. A total of 72 Nelore heifers (32 HFC and 40 LFC) and 48 Angus heifers (21 HFC and 27 LFC) were selected and the DNA was extracted from blood and hair bulb. Genotyping was done using the Illumina Bovine HD 770K BeadChip. The GWAS analysis showed 181 and 201 SNPs with genotype/phenotype association (P ≤ 0.01) in Nelore and Angus heifers, respectively. Functional enrichment analysis was performed on candidate genes that were associated with SNPs. A total of 97 genes were associated to the 181 SNPs in the Nelore heifers and the functional analysis identified genes (ROBO1 and SLIT3) in the ROBO-SLIT pathway that can be involved in the control of germ cell migration in the ovary as it is involved in lutheal cell migration and fetal ovary development. In the Angus heifers, 57 genes were associated with the 201 SNPs, highlighting Fribilin 1 (FBN1) gene, involved in regulation of growth factors directly involved in follicle activation and development. In summary, GWAS for Nelore and Angus heifers showed SNPs associated with higher follicle count phenotype. Furthermore, these findings offer valuable insights for the further investigation of potential mechanism involved in follicle formation and development, important for breeding programs for both breeds.
RESUMEN
The present study aimed to describe the disposition and amount of circumvallate lingual papillae in Saanen goats using 26 goat tongues from the Laboratory of Animal Anatomy and Morphology of the Federal Rural University of Rio de Janeiro, which were previously fixed in 10% formaldehyde solution for later dissection of tongues that were removed from the oral cavity for the necessary studies. After identification, morphometric data were tabulated and submitted to statistical analysis for further description. In descriptive statistics, the mean of 17.85 was close to the median of 17.5 and the overall dispersion (standard deviation) was relatively low (2.24), which represents a coefficient of variation (CV) of 12.8 %. Confidence interval for mean with 95 % with 99 % confidence was also performed. Regarding the distribution of papillae, 21 tongues (80.7 %) of the total samples were located on the side of the tongue root, 3 tongues (11.5%) were distributed on the side of the tongue root, migrating toward the tongue median sagittal plane, 1 tongue (3.8 %) was located in the concave position on the side of the tongue root and 1 tongue (3.8 %) was disposed on the sides of the tongue, with some papillae arranged close to the region of the tongue median sagittal plane.
O presente estudo objetivou descrever a disposição e quantidade das papilas circunvaladas das línguas de cabras da raça Saanen, utilizando línguas de 26 cabras da raça, provenientes do Laboratório de Anatomia e Morfologia Animal da Universidade Federal Rural do Rio de Janeiro, que foram fixadas previamente em solução de formaldeído a 10% para posterior dissecção das línguas que foram retiradas da cavidade oral para os estudos necessários. Após identificação, os dados morfométricos foram tabulados e submetidos à análise estatística para posterior descrição. Na estatística descritiva, a média de 17,85 ficou próxima da mediana de 17,5 e a dispersão geral (desvio padrão) foi relativamente baixo (2,24) que representa um coeficiente de variação (CV) de 12,8%. Também foi feito o Intervalo de Confiança para a média com 95% e com 99% de confiança. Em relação a distribuição, à forma de distribuição das papilas, 21 línguas (80,7%) do total de amostras apresentadas encontravam-se dispostas nas laterais da raiz da língua, 3 línguas (11,5%), apresentavam distribuição nas laterais da raiz da língua migrando em direção ao plano sagital mediano da língua, 1 língua (3,8%) apresentava-se disposta de forma côncava nas laterais da raiz da língua e 1 língua (3,8%) encontrava-se disposta nas laterais da língua, com algumas papilas dispostas próximo a região do plano sagital mediano da língua.