RESUMEN
The aim of this study was to prepare cellulose nanowhiskers (CNWs) from wastepaper powder (WPP), as an environmentally friendly approach for obtaining the source material, which is a highly available and low-cost precursor for cellulose nanomaterial processing. Acid hydrolysis and calcification treatments were employed for extraction of CNWs and preparation of novel phospho-calcified cellulose nanowhiskers (PCCNWs). CNWs and PCCNWs were analyzed through optical microscopy (OM), scanning electron microscopy (SEM), Fourier-transformed infrared spectra (FTIR) and X-ray diffraction analysis (XRD). Cell behaviors in the presence of CNWs and PCCNWs were studied by MTT assay and live-dead staining. Finally, the effect of these particles on osteogenic differentiation of stem cells was evaluated based on alkaline phosphatase activity (ALP), calcium mineralization as well as von Kossa and alizarin red staining. Based on the results, PCCNWs had a positive effect on osteogenic differentiation of human mesenchymal stem cells (hMSCs) and can be used for developing new approaches for bone tissue engineering.
Asunto(s)
Diferenciación Celular , Celulosa/química , Células Madre Mesenquimatosas/citología , Osteogénesis , Papel , Células Cultivadas , HumanosRESUMEN
Nowadays, the discovery of cell behaviors and their responses in communication with the stem cell niches and/or microenvironments are one of the major topics in tissue engineering and regenerative medicine. In this study, incorporated organic-inorganic polyurethane (PU) nanocomposites were prepared for better understanding of cell signaling and the effect of magnetite nanoparticles on cell proliferation and cell responses. The properties of PU-IONs were evaluated by fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), atomic-force microscopy (AFM), differential scanning calorimetry (DSC), X-ray diffraction (XRD) and electrochemical impedance spectroscopy (EIS). The presence of the iron oxide nanoparticles (IONs) affects on the properties of polyurethane nanocomposites such as bulk morphology, mechanical, electrochemical, and biological properties. The electrical conductivity and hydrophilicity of PU-IONs were improved by increasing the magnetite nanoparticles; therefore water absorption, biodegradation and cell viability were changed. The biocompatibility of PU-IONs was investigated by MTT assay, cell attachment and cell staining. According to the results, the magnetite polyurethane nanocomposites could be a potential choice for cell therapy and tissue engineering, especially nerve repair.