RESUMEN
In this study, the aligned (A) and randomly oriented (R) polycaprolactone (PCL-A and PCL-R) and PCL/collagen (PCL/Col-A and PCL/Col-R) nanofibers were electrospun onto smooth PCL membranes (PCLMs) prepared by solvent casting. In order to investigate the effects of chemical composition and nanotopography of fibrous surfaces on proliferation and on neural differentiation of mesenchymal stem cells (MSCs), adipose and bone marrow-derived rat MSCs (AdMSCs and BMSCs) were cultivated in suitable media i.e. inducing medium containing basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF), and cell maintenance medium (CMM). BMSCs adhered and proliferated on all nanofibrous membranes more efficiently than AdMSCs. PCL/Col-A was found as the most convenient surface supporting proliferation in both cell types. Immunofluorescence staining indicated that BMSCs and AdMSCs are prone for differentiation to oligodendrocytes more than they differentiate to other neuronal cell types. PCL-A nanofibrous membranes supported differentiation of MSCs to O4(+) (an oligodendrocytes surface antigen) cells in both culture media. The intensity of immunoreactivity of O4(+) cells differentiated from BMSCs on PCL-A was highest when compared with the other groups (p < 0.001). Some BIII-T signed neural cells were investigated on PCL-A nanofibrous membranes, but the intensity of immunoreactivity was lower than that of O4(+) cells. In conclusion, this study can be evaluated to establish the cell therapy strategies in neurodegenerative disorders, which are relevant to oligodendrocyte abstinence using BMSCs or AdMSCs on aligned nanofibrous membranes.
Asunto(s)
Células de la Médula Ósea/citología , Células Madre Mesenquimatosas/citología , Poliésteres/química , Animales , Adhesión Celular , Diferenciación Celular , Proliferación Celular , Supervivencia Celular , Colágeno/química , Medios de Cultivo/química , Factor de Crecimiento Epidérmico/metabolismo , Factor 2 de Crecimiento de Fibroblastos/metabolismo , Citometría de Flujo/métodos , Microscopía Electrónica de Rastreo/métodos , Microscopía Fluorescente/métodos , Nanofibras/química , Neuronas/metabolismo , Ratas , Sales de Tetrazolio/química , Tiazoles/química , Ingeniería de Tejidos/métodos , Andamios del Tejido/químicaRESUMEN
OBJECTIVE: The purpose of this in vitro study was to investigate antimicrobial activity of calcium hydroxide (CH) in combination with glycerin, chlorhexidine gluconate (CHX), cetrimide, or distilled water against Enterococcus faecalis and Candida albicans. STUDY DESIGN: Standard holes in the cultivated agar plates were filled with one of the CH preparations and control agents. The zones of microbial inhibition were measured after incubation period. RESULTS: The CH preparations with glycerin and CHX demonstrated more antifungal activity than CH preparations with cetrimide and distilled water. The CH-glycerin preparations had no effect against E. faecalis, and CH-CHX preparation was the most effective medication. CONCLUSION: Antimicrobial activity of CH may change with the type of the vehicle and against different microorganisms. Enterococcus faecalis was more resistant than C. albicans to CH preparations.
Asunto(s)
Antiinfecciosos Locales/farmacología , Hidróxido de Calcio/farmacología , Candida albicans/efectos de los fármacos , Enterococcus faecalis/efectos de los fármacos , Vehículos Farmacéuticos/farmacología , Materiales de Obturación del Conducto Radicular/farmacología , Antiinfecciosos Locales/química , Hidróxido de Calcio/química , Cetrimonio , Compuestos de Cetrimonio/química , Compuestos de Cetrimonio/farmacología , Clorhexidina/análogos & derivados , Clorhexidina/química , Clorhexidina/farmacología , Recuento de Colonia Microbiana , Combinación de Medicamentos , Glicerol/química , Glicerol/farmacología , Vehículos Farmacéuticos/química , Materiales de Obturación del Conducto Radicular/químicaRESUMEN
OBJECTIVE: The aim of this study was to observe the colonization pattern of C. albicans on treated and untreated radicular dentin. STUDY DESIGN: Root sections of 10 human mandibular premolar teeth were longitudinally separated into halves. The 20 halves were separated into 2 groups and each half served as its own control. In Group 1, only gross pulpal remnants were removed with pliers. Root canal walls in the corresponding 102 halves (Group 2) were instrumented with Gates-Glidden burs and treated with sequential use of 15% EDTA solution for 3 minutes and 2.5% NaOCl solution for 3 minutes. Finally, all teeth were washed with distilled water. Each specimen was placed individually in each well of a 24-well cell culture plate. After the assembly was sterilized with ethylene-oxide, the root canal of each specimen was inoculated with 20 microL of C. albicans (1-1.5 x 10(6) cfu/mL) that was kept in place for 24 hours for initial attachment. Then, 2 mL of SDB was added to each well and the assembly was placed in an incubator at 37 degrees C for 10 days. Following the incubation period, the specimens were washed, fixed, dehydrated, and processed for scanning electron microscopy. RESULTS: C. albicans was present on the root canal surfaces of all specimens; however, the colonization pattern was different. In the untreated group, the main growth pattern was a dense mass of yeast cells forming biofilm layers while hyphal structures were not common. On the other hand, pseudohyphae invaded all root canal surfaces in Group 2 and yeast cells were occasionally observed. CONCLUSION: The treatment procedures of root canal dentin have a strong influence on the colonization pattern of C. albicans. This fact should be considered when planning and evaluating in vitro Candida adhesion and/or penetration studies.