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In the present study we measured the amount of 8-oxo-2'-deoxyguanosine (8-oxo-dG) in DNA isolated from lymphocytes of arteriosclerotic patients undergoing ozonetherapy. Treatment of the patients with therapeutic concentration of ozone caused a significant increase over the control value in the amount of 8-oxo-dG of DNA isolated from their lymphocytes. However, only three out of six patients examined responded positively to the treatment in terms of the base damage. The increases varied among patients, and were in the range of 100-450%. This interindividual difference may at least be partly explained by recently demonstrated heritable susceptibility to ozone.

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From 1981 to 1986 in the Department of Maxillofacial Surgery, Institute of Stomatology, Medical Academy in Warsaw 85 patients were treated for carcinoma of the upper gingiva and palate. The follow-up of the patients after treatment was from 3 to 7 years. The obtained results of the treatment were analysed depending on the degree of differentiation of neoplasms and their biological aggressiveness and the therapeutic method used. It was found that the results of the treatment of upper gingiva carcinoma were not satisfactory. The best results were obtained using surgical treatment followed by radiotherapy. A positive result of the treatment without symptoms of recurrence was achieved in 21 cases (24.7%).

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In a period of 3 years, from 1984 to 1986 in the Department of Maxillofacial Surgery, Institute of Stomatology, Medical Academy in Warsaw 442 patients were treated for zygomatico-maxillary fractures, in 101 cases the treatment was conservative, and 341 were treated surgically by various operative methods. The aim of the study was assessment of late effectiveness of the surgical methods used most frequently in zygomatico-maxillary fractures with reposition of skeletal fragments either with one-pronged hook introduced percutaneously or from the side of the lumen of the maxillary sinus. The criterion of effectiveness of both methods was accepted to be functional assessment of the visual system and the stomatognathic system, and the condition of sensory function in the area of innervation by the suborbital nerve. Control examinations were done 2-5 years after operations confirming the both methods were successful in 90% of the treated group. A more detailed analysis of these methods is not possible since the qualification for these operations differentiated essentially both groups of patients.

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In the period of 3 years, from 1984 to 1986 in the Department of Maxillofacial Surgery, Institute of Stomatology, Medical Academy in Warsaw 230 patients were treated for maxillary fractures. Late results of this treatment were assessed in 102 patients coming for control examinations in 1988. In the assessment of the therapeutic results the following features were considered: facial symmetry, proportions of three facial segments, functions of the visual system and stomatognathic system, nasal patency, and sensory disturbances in the area innervated by the infraorbital nerves. On the basis of this late assessment of the results of treatment of these fractures it was found that the definite morphological-functional and aesthetic results were unsatisfactory in cases of Le Fort III and II type maxillary fractures. This points out that the therapeutic methods used as yet are not sufficiently satisfactory and other methods should be evolved.

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During 3 years 1246 patients were treated for mandibular fractures, 828 of them were treated by conservative-orthopaedic methods while in 418 patients the displaced mandibular fragments were reduced and fixed surgically using various methods. The purpose of the assessment was a group of 319 patients in whom the fragments were reduced and fixed with ossous sutures. A good result of treatment was achieved after this operation in 291 cases (91.2%). In 28 patients (8.8%) postoperative complications developed requiring reoperation.

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In a group of 1246 patients treated in the Department of Maxillofacial Surgery-Institute of Stomatology, Medical Academy in Warsaw, in a period of 3 years (1984-1986) late results of treatment of mandibular fractures were assessed. In the assessment the range of mandible abduction, function of temporomandibular joints, occlusal abnormalities, the sensory function in the area innervated by the mental nerves and the condition of posttraumatic scars changing the morphology and aesthetic appearance of the face were considered. Limitation of mandibular abduction after treatment was found in 39 cases. Functional disturbances of temporomandibular joints developed in 58 cases, and various occlusal complications were found in 130 out of 1246 patients. In 56 patients various sensory loss was noted in the innervation area of the mental nerves. Posttraumatic scars in 89 patients affected in various degree facial morphology and aesthetic appearance.

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Oil induced mice peritoneal cells were stored at -196 degrees C for 3 months, using dimethyl sulfoxide (DMSO) as a cryoprotective medium. Recovery counts upon thawing were about 90%. Macrophages retained their phagocytic activity of SRBC in 89% and there were no significant differences in spontaneous migration, expression of membrane immune receptors and non-specific cytotoxicity of frozen-thawed cells compared with the controls.

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