RESUMEN
Error growth in spatiotemporal chaotic systems is investigated by analyzing the interplay between temporal and spatial dynamics. The spatial correlation and localization of relative fluctuations grow and decay indicating two different regimes, before and after saturation by nonlinear effects. This general behavior is shown to hold both in simple coupled map lattices and in global weather models. This explains the increasing or decreasing trends previously observed in the exponential growth rate of these spatiotemporal systems.
Asunto(s)
Dinámicas no Lineales , Tiempo (Meteorología) , Algoritmos , Predicción , Modelos Estadísticos , Factores de TiempoRESUMEN
Mycotoxins are toxic secondary metabolites produced by a number of different fungi, and can be present in a wide range of food and feed commodities including cereal grains, oil seeds, dried fruits, apple juice, wine and meat products from animals fed contaminated meal. Many mycotoxins are highly resistant, and survive food processing, and therefore enter the food chain and provide a threat to human health. The optical waveguide lightmode spectroscopy (OWLS) technique has been applied to the detection of Aflatoxin and Ochratoxin in both competitive and in direct immunoassays. After immobilizing the antibody or antigen conjugate for the direct or indirect measurement, respectively, the sensor chip was used in flow-injection analyser (FIA) system. When using non-competitive method, sensor responses were obtained first only at analyte concentrations of 5-10 ng ml(-1). In both cases, the responses were very unstable. For competitive sensor investigation with the sensitized chip first the optimal dilution rate of monoclonal antibodies was determined, for the measurement of Ochratoxin A and Aflatoxin B1 the monoclonal antibody stock solution was diluted to 1 microg ml(-1) and to a 1:400 dilution, respectively. During the competitive measurement standard solutions were mixed with monoclonal antibodies at the appropriate concentration, the mixture was incubated for 1 min and injected into the OWLS system. The sensitive detection range of the competitive detection method was between 0.5 and 10 ng ml(-1) in both cases. After the establishment of the indirect method, barley and wheat flour samples were measured, and the results were in good correlation by those measured by enzyme linked immuno-sorbent assay (ELISA). Regression coefficient between the two methods for Ochratoxin and Aflatoxin was determined as 0.96 and 0.89, respectively.
Asunto(s)
Aflatoxina B1/análisis , Técnicas Biosensibles/instrumentación , Tecnología de Fibra Óptica/instrumentación , Inmunoensayo/instrumentación , Ocratoxinas/análisis , Análisis Espectral/instrumentación , Técnicas Biosensibles/métodos , Diseño de Equipo , Análisis de Falla de Equipo , Inmunoensayo/métodos , Reproducibilidad de los Resultados , Sensibilidad y EspecificidadRESUMEN
By incorporating a grating in a planar optical waveguide one creates a device with which the spectrum of guided lightmodes can he measured. When the surface of the waveguide is exposed to different solutions, the peaks in the spectrum shift due to molecular interactions with the surface. Optical waveguide lightmode spectroscopy (OWLS) is a highly sensitive technique that is capable of real-time monitoring of these interactions. Since this integrated optical method is based on the measurement of the polarizability density (i.e., refractive index) in the vicinity of the waveguide surface, radioactive, fluorescent or other kinds of labeling are not required. In addition, measurement of at least two guided modes enables the absolute mass of adsorbed molecules to be determined. In this article, the technique will be described in some detail, and applications from different areas will be discussed. Selected examples will be presented to demonstrate how monitoring the modification of different metal oxides with polymers and the response of the coated oxides to biofluids help in the design of novel biomaterials; how OWLS is useful for accurate bioaffinity sensing, which is a key issue in the development of new drugs; and how the quantitative study of protein-DNA/RNA and cell surface interactions can enhance the understanding of processes in molecular and cellular biology.
Asunto(s)
Técnicas Biosensibles/instrumentación , Óptica y Fotónica/instrumentación , Adsorción , Materiales Biocompatibles/química , ADN/química , Cinética , Membrana Dobles de Lípidos/química , Sustancias Macromoleculares , Ensayo de Materiales , Membranas Artificiales , Unión Proteica , Proteínas/química , Propiedades de SuperficieRESUMEN
Methods have been developed which enable attachment amino and epoxy groups to the surface of integrated optical wave-guide sensors for immunosensor applications. The SiO2-TiO2 surfaces were modified by use of the trifunctional silane reagents gamma-aminopropyltriethoxysilane (APTS) and gamma-glycidoxypropyltrimethoxysilane (GOPS) in organic and/or in inorganic phases. Silanization methods were optimized taking into consideration the concentration of silane reagent used and the temperature and time of reaction. To evaluate the layers formed, immobilization experiments were undertaken on the modified surfaces using the bovine serum albumin (BSA)-anti-BSA IgG antibody model molecule pair. The regenerability of the sensitized surfaces was also studied.