RESUMEN

OBJECTIVE: The objective of the study is to identify phase coupling patterns that are shared across subjects via a machine learning approach that utilises source space magnetoencephalography (MEG) phase coupling data from a working memory (WM) task. Indeed, phase coupling of neural oscillations is putatively a key factor for communication between distant brain areas and is therefore crucial in performing cognitive tasks, including WM. Previous studies investigating phase coupling during cognitive tasks have often focused on a few a priori selected brain areas or a specific frequency band, and the need for data-driven approaches has been recognised. Machine learning techniques have emerged as valuable tools for the analysis of neuroimaging data since they catch fine-grained differences in the multivariate signal distribution. Here, we expect that these techniques applied to MEG phase couplings can reveal WM-related processes that are shared across individuals. APPROACH: We analysed WM data collected as part of the Human Connectome Project. The MEG data were collected while subjects (n = 83) performed N-back WM tasks in two different conditions, namely 2-back (WM condition) and 0-back (control condition). We estimated phase coupling patterns (multivariate phase slope index) for both conditions and for theta, alpha, beta, and gamma bands. The obtained phase coupling data were then used to train a linear support vector machine in order to classify which task condition the subject was performing with an across-subject cross-validation approach. The classification was performed separately based on the data from individual frequency bands and with all bands combined (multiband). Finally, we evaluated the relative importance of the different features (phase couplings) for classification by the means of feature selection probability. MAIN RESULTS: The WM condition and control condition were successfully classified based on the phase coupling patterns in the theta (62% accuracy) and alpha bands (60% accuracy) separately. Importantly, the multiband classification showed that phase coupling patterns not only in the theta and alpha but also in the gamma bands are related to WM processing, as testified by improvement in classification performance (71%). SIGNIFICANCE: Our study successfully decoded WM tasks using MEG source space functional connectivity. Our approach, combining across-subject classification and a multidimensional metric recently developed by our group, is able to detect patterns of connectivity that are shared across individuals. In other words, the results are generalisable to new individuals and allow meaningful interpretation of task-relevant phase coupling patterns.

Asunto(s)

Magnetoencefalografía , Memoria a Corto Plazo , Encéfalo , Humanos , Neuroimagen , Máquina de Vectores de Soporte

RESUMEN

Magnetoencephalography has gained an increasing importance in systems neuroscience thanks to the possibility it offers of unraveling brain networks at time-scales relevant to behavior, i.e., frequencies in the 1-100 Hz range, with sufficient spatial resolution. In the first part of this review, we describe, in a unified mathematical framework, a large set of metrics used to estimate MEG functional connectivity at the same or at different frequencies. The different metrics are presented according to their characteristics: same-frequency or cross-frequency, univariate or multivariate, directed or undirected. We focus on phase coupling metrics given that phase coupling of neuronal oscillations is a putative mechanism for inter-areal communication, and that MEG is an ideal tool to non-invasively detect such coupling. In the second part of this review, we present examples of the use of specific phase methods on real MEG data in the context of resting state, visuospatial attention and working memory. Overall, the results of the studies provide evidence for frequency specific and/or cross-frequency brain circuits which partially overlap with brain networks as identified by hemodynamic-based imaging techniques, such as functional Magnetic Resonance (fMRI). Additionally, the relation of these functional brain circuits to anatomy and to behavior highlights the usefulness of MEG phase coupling in systems neuroscience studies. In conclusion, we believe that the field of MEG functional connectivity has made substantial steps forward in the recent years and is now ready for bringing the study of brain networks to a more mechanistic understanding.