RESUMEN
We recently discovered a novel hantavirus, Sangassou virus, in Guinea, West Africa. Using enzyme-linked immunosorbent assays followed by confirmatory and serotyping assays, we retrospectively detected hantavirus antibodies in 3 (4.4%) of 68 patients with fever of unknown origin in Sangassou village, Forest Guinea. A population-based survey in Forest Guinea (n = 649) found the prevalence of hantavirus antibodies to be 1.2%. Specific neutralizing antibodies against Sangassou virus were demonstrated in serum samples from 2 patients and in 2 serum samples of the serosurvey. Our data allow us to conclude that hantavirus infections may be a significant unrecognized medical problem in at least this part of Africa.
Asunto(s)
Anticuerpos Antivirales/sangre , Infecciones por Hantavirus/sangre , Orthohantavirus/aislamiento & purificación , Anticuerpos Neutralizantes/sangre , Niño , Ensayo de Inmunoadsorción Enzimática , Femenino , Guinea/epidemiología , Orthohantavirus/inmunología , Infecciones por Hantavirus/epidemiología , Infecciones por Hantavirus/inmunología , Infecciones por Hantavirus/transmisión , Humanos , Inmunoglobulina G/sangre , Inmunoglobulina M/sangre , Masculino , Estudios Retrospectivos , Estudios SeroepidemiológicosRESUMEN
Based on empiric surveillance data, the incidence of human Lassa fever (LF) cases in Guinea and other West African countries has been reported to increase during the dry season compared to the rainy season. To investigate possible links with the ecology of the rodent reservoir of the virus, we conducted a 2-year longitudinal survey of Mastomys natalensis in a region of high human Lassa virus (LASV) seropositivity in Guinea. Standardized rodent trapping with similar trapping efforts between seasons was performed in three villages and 53.5% (601/1123) of the animals were identified as M. natalensis using morphometric and molecular criteria. Mean trapping success (TS) of M. natalensis was always higher inside houses than in proximal cultivations. In the dry season, mean TS increased 2-fold inside houses and decreased up to 10-fold outside (p < 0.0001), suggesting aggregation of rodents inside houses due to restricted food supply. 14.5% (80/553) of M. natalensis were tested positive for Lassa virus by reverse transcription-polymerase chain reaction (RT-PCR; range, 5%-30%) and prevalence of the virus was two to three times higher in rodents captured in the rainy season than in the dry season (p < 0.05). Inside houses, however, the LASV prevalence fluctuated nonsignificantly with season. These data suggest that in Guinea the risk of LASV transmission from rodents to humans is present both in the rainy and the dry season, reflected by the occurrence of LF cases throughout the year. In the dry season, however, the increased risk of humans encountering Mastomys and their excreta inside of houses may result in an increase of human Lassa fever cases.
Asunto(s)
Fiebre de Lassa/transmisión , Fiebre de Lassa/veterinaria , Virus Lassa/aislamiento & purificación , Murinae/virología , Enfermedades de los Roedores/transmisión , Zoonosis , Animales , Reservorios de Enfermedades/veterinaria , Reservorios de Enfermedades/virología , Guinea/epidemiología , Humanos , Fiebre de Lassa/epidemiología , Prevalencia , Lluvia , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Enfermedades de los Roedores/epidemiología , Estaciones del AñoRESUMEN
PCR screening of 1,482 murid rodents from 13 genera caught in 18 different localities of Guinea, West Africa, showed Lassa virus infection only in molecularly typed Mastomys natalensis. Distribution of this rodent and relative abundance compared with M. erythroleucus correlates geographically with Lassa virus seroprevalence in humans.