RESUMEN
The extent to which pancreatic juice can contaminate bile collected from a rat with a biliary fistula has been investigated by cannulating the bile duct proximal to either the duodenum or the liver, and by stimulating pancreatic flow with secretin. Bile collected via a fistula proximal to the duodenum showed marked pancreatic contamination. Thus, bile collected via a fistula proximal to the duodenum has a higher flow rate, a greater total protein and amylase content and a different polypeptide profile than bile collected via a fistula proximal to the liver. The phospholipid content also differed in that phosphatidylcholine was converted enzymically to lysophosphatidylcholine. Secretin increased bile flow and the biliary output of total protein and amylase when the fistula was proximal to the duodenum, but had no effect upon these parameters when the fistula was proximal to the liver, or in the isolated perfused rat liver.
Asunto(s)
Bilis/análisis , Fosfolípidos/metabolismo , Proteínas/análisis , Amilasas/análisis , Animales , Bilis/efectos de los fármacos , Bilis/metabolismo , Conductos Biliares/cirugía , Cromatografía en Capa Delgada , Fístula , Hígado/metabolismo , Lisofosfatidilcolinas/análisis , Péptidos/análisis , Fosfolípidos/análisis , Ratas , Ratas Endogámicas , Secretina/farmacologíaRESUMEN
In vitro studies on mitogenic stimulation of lymphocytes from a panel of normal volunteers revealed no transformation in response to the presence of dextran 40, 70, 110 or 150 at concentrations ranging from 0.8 to 8,000 microgram/ml. High molecular weight native dextran B512 was mitogenic in 1 individual only. In addition, neither Leuconostoc-derived nor fermentation medium-derived moieties, sometimes present in clinical dextrans, were implicated as lymphocyte mitogens. It is concluded on the basis of these findings that clinical dextrans of average molecular weight 40,000--150,000 are not B- or T-cell mitogens.