RESUMEN
A new method of labelling short ragweed pollen antigens with carbon-14 is described. The known ragweed antigens AgE, Ra3, Ra5 and several other antigens are radiolabelled. These components are biologically active, have a high specific radioactivity and a long half-life.
Asunto(s)
Antígenos , Radioisótopos de Carbono , Marcaje Isotópico/métodos , Polen , Trypanosoma cruzi , Antígenos/análisis , Antígenos/aislamiento & purificación , Liberación de Histamina , Extractos Vegetales/inmunología , Extractos Vegetales/normas , Polen/inmunologíaAsunto(s)
Células HeLa/metabolismo , Calor , Línea Celular , Nucléolo Celular , Núcleo Celular/análisis , Cromosomas/análisis , Citoplasma/efectos de los fármacos , Dactinomicina/farmacología , Retículo Endoplásmico/análisis , Fibroblastos/citología , Aparato de Golgi/análisis , Histocitoquímica , Humanos , Lisosomas/análisis , Métodos , Microtúbulos , Mitocondrias/análisis , Ácidos Nucleicos/análisis , Pepsina A/farmacología , ARN Neoplásico/biosíntesis , Ribonucleasas/farmacología , Ribosomas/análisis , Ribosomas/efectos de los fármacos , Factores de Tiempo , Tritio , UridinaAsunto(s)
Antibióticos Antineoplásicos/farmacología , Leucosis Aviar/metabolismo , ARN Viral/biosíntesis , Animales , Antibióticos Antineoplásicos/uso terapéutico , Leucosis Aviar/patología , Virus de la Leucosis Aviar , Centrifugación por Gradiente de Densidad , Embrión de Pollo , Fibroblastos/metabolismo , Técnicas In Vitro , ARN Neoplásico/biosíntesis , Ribosomas/metabolismoRESUMEN
Strain MC29 avian leukosis (myelocytomatosis) virus induced infection, elaboration of virus, and morphological alteration in chick embryo cells in vitro. Virus liberation began within 18 hr, morphological change was detectable at about 40 hr, and the cultures could be completely altered within 80 hr after infection. Altered cells were about half the volume and grew at approximately twice the rate of uninfected elements. The output of virus estimated by electron microscopy was about 140 particles per cell per hr. Deoxyribonucleic acid remained constant, but ribonucleic acid increased in both infected and control cells in adjustment to culture environment. The rates of uptake and incorporation of (3)H-uridine and the incorporation of (3)H-thymidine increased in the infected cells with onset of morphological change but were unaffected by processes of infection and virus elaboration per se. Incorporation of a (14)C-amino acid mixture was slightly greater in the infected than in control cells. The speed of continuity of infection and massive morphological alteration constitute a unique response to avian tumor viruses, and the system gives promise of singular value for detailed studies of the processes of infection and morphological change.