RESUMEN
Spontaneous luminol-dependent chemiluminescence (CL) of thrombocytes was studied in 12 patients with hemorrhagic fever with renal syndrome (HFRS) caused by Puumala virus. CL in the acute phase of HFRS (median, 61 mV/10 sec; range, 22-91) was higher than that of healthy controls (median, 22 mV/10 sec; range, 18-29; P < 0.01) and patients with acute hepatitis B infection accompanied by thrombocytopenia (median, 33 mV/10 sec; range, 13-45; P < 0.01). CL of thrombocytes in the convalescence phase of HFRS (median, 31 mV/10 sec; range, 12-80) was significantly lower than that during the acute phase (P < 0.01). Neither was there a difference in CL response between HFRS patients with or without thrombocytopenia, but both groups produced a higher CL response than that of the control groups. Both the complement-containing and complement-free sera from acute HFRS patients (final dilutions: range 1:10-1:2560) exhibited no effect on CL response of normal thrombocytes. A possible role of increased oxygen radicals production by thrombocytes in pathogenesis of HFRS is discussed.
Asunto(s)
Plaquetas/química , Plaquetas/metabolismo , Fiebre Hemorrágica con Síndrome Renal/sangre , Luminol/farmacología , Especies Reactivas de Oxígeno/análisis , Animales , Chlorocebus aethiops , Humanos , Mediciones Luminiscentes , Neutrófilos/fisiología , Valores de Referencia , Células VeroRESUMEN
Spontaneous and Bacillus anthracis induced luminol-dependent chemiluminescence of neutrophils was studied in six patients with the cutaneous form of anthrax. Spontaneous chemiluminescence in anthrax was decreased compared to the healthy controls (p < 0.05). B. anthracis, opsonized by complement-containing sera from patients, induced chemiluminescence in neutrophils from control donors but not from patients. B. anthracis, opsonized by complement-free sera from the patients, did not cause an increase in chemiluminescence response in either the patient or the control neutrophils. Also, B. anthracis, opsonized with normal sera and nonopsonized, did not induce chemiluminescence in either patient or control neutrophils. It was concluded that oxidative metabolism by neutrophils is impaired in anthrax, whereas the functional capacity of antibodies seems to be unaffected.