RESUMEN
Gliocladium roseumis a successful antagonist of Botrytis cinerea and is considered to have the major potential for biocontrol of the pathogen in cropping systems. In order to elucidate the optimal moment of the day to apply the biological control agent, geranium plants were inoculated until run off with a suspension containing 10 e7 conidia of G. roseum + Tritón 100X. The inoculation times were 9 am, 12 am, 3 pm and 6 pm. The number cfu per cm(2) of leaves at inoculation time (time 0) and at 3, 6 y 9 h after inoculation were estimated. Then, the inoculated plants were kept in a Plexiglas, humidity chamber for 24 h. Discs of 8 mm in diameter were cut off from the inoculated leaves and put then into a paraquat medium to examine the development of G. roseum. According to the results dry period length after inoculation time, shows a significant decrease in number of cfu by cm(2) of leaf. No difference was observed between 3, 6 y 9 h after inoculation time.
RESUMEN
The influence of the physical exercise at high altitude on the endocrine function was studied in 8 normal native men of sea level and in 8 natives men of high altitude. The sea level dwellers were studied both, at sea level, during an acute exposure to low barometric pressure and after 3 months of acclimatization to altitudes over 3,500 meters above the sea level. The experiments at high altitude were conducted at an altitude of 4,500 meters above the sea level. Two types of exercise were carried out, sub-maximal and maximal, at fasting state, between 8 and 10 a.m. During an acute exposure to altitude the physical exercise produced a marked rise of glucose, cortisol and growth hormone and a fall in the insulin content of plasma. In the sea level dwellers, acclimatized to altitude during 3 months, an elevation of growth hormone was observed only during maximal physical effort. Marked variation in glucose and cortisol were observed during both types of exercise. This shows that in these subjects some adaptative changes have ocurred but of lesser extent as those observed in altitude natives. In the high altitude native higher basal concentrations of growth hormone and glucagón as well as a lower glucose concentration in blood, were found. During exercise the high altitude dweller showed no significant changes in somatotropin, meanwhile an important elevation of cortisol occurred. These findings indicate that the high altitude native has metabolic and endocrine responses to exercise similar to those found in well fitted atletes of sea level. The exposure to altitude provoked a rise in glucagon concentration directly proportional to the time of exposition ot altitude. The physical exercise did not elucidate any change in the glucagon content of blood.