RESUMEN
A bone marrow transplant (BMT) is one kind of standard treatment modality in advanced hemato-oncology. In order to set up a BMT unit, one of the important steps before starting a clinical program is to evaluate the cryopreservation procedure for stem cell storage. Twenty one bags of buffy coat were used to be the testing specimens. They were processed and frozen according to cryopreservation protocol and kept in liquid nitrogen for 2 weeks. The evaluation process was carried out with a lymphocyte proliferation test together with trypan blue staining. By measuring the optical density of each lymphocyte containing well after stimulation, the lymphocyte proliferation value (LPV) could be obtained. When comparing them before and after cryopreservation, the LPV was 2.064 ± 0.379 (mean ± SD) and 1.913 ± 0.546, (p = 0.314), respectively. At 2 weeks after cryopreservation, comparing between the frozen group and the unfrozen control, the LPV was 1.913 ± 0.546 and 0.486 ± 0.453, (p < 0.05), respectively. The LPV showed clear efficacy of the procedure, especially for preserving the cellular proliferation function. Our model of the cryopreservation procedure evaluation at pre-clinical phase by use of a buffy coat and lymphocyte proliferation test seems feasible for newly-established small BMT units. With these results, clinical transplantations can be performed with more confidence.
Asunto(s)
Células de la Médula Ósea/metabolismo , Trasplante de Médula Ósea , Criopreservación , Congelación , Neoplasias Hematológicas/terapia , Células de la Médula Ósea/patología , Proliferación Celular , Células Cultivadas , Criopreservación/métodos , Estudios de Factibilidad , Congelación/efectos adversos , Neoplasias Hematológicas/patología , Humanos , Coloración y Etiquetado , Azul de Tripano/metabolismoRESUMEN
Diabetic patients with poorly controlled blood glucose have frequent and persistent bacterial infections particularly those infecting the skin, such as Staphylococcus aureus and S. epidermidis. The function of phagocytes of diabetic patients is believed to be impaired due to hyperglycemia, leading to suboptimal immune response to clear acute infection. The present study investigated interleukin (IL)-1beta expression by diabetic patients' monocytes (n = 22) experimentally infected with S. aureus compared with that from healthy subjects (n = 30). In addition, the in vitro effect of hyperglycemia on IL-1beta expression by monocytes from normal subjects (n = 18) stimulated with S. aureus and S. epidermidis was investigated. Monocytes from diabetic patients, stimulated or not with S. aureus, express significantly lower levels of IL-1beta than those from healthy subjects. In vitro hyperglycemia did not affect IL-1beta expression by unstimulated monocytes. However, at the same levels of glucose normal monocytes stimulated with S. aureus produce significantly higher IL-1beta than those stimulated with S. epidermidis. These findings suggest that diabetic patients have abnormally lower IL-1beta expression and hyperglycemia is related to abnormal expression of IL-1beta by monocytes, which could lead to enhanced susceptibility to infection by the more virulent bacteria.
Asunto(s)
Complicaciones de la Diabetes/inmunología , Hiperglucemia/inmunología , Interleucina-1beta/inmunología , Infecciones Cutáneas Estafilocócicas/inmunología , Adulto , Complicaciones de la Diabetes/microbiología , Humanos , Hiperglucemia/complicaciones , Hiperglucemia/etiología , Técnicas In Vitro , Persona de Mediana Edad , Monocitos/inmunología , Monocitos/fisiología , Fagocitos/inmunología , Fagocitos/fisiología , Infecciones Cutáneas Estafilocócicas/microbiología , Staphylococcus aureus/inmunología , Staphylococcus aureus/aislamiento & purificación , Staphylococcus epidermidis/inmunología , Staphylococcus epidermidis/aislamiento & purificación , TailandiaRESUMEN
PROBLEM: JEG-3 choriocarcinoma cell line has previously been reported to express a receptor for interleukin (IL)-17. The involvement of IL-17 in the production of progesterone and human chorionic gonadotropin by placental trophoblast has not been investigated. METHOD OF STUDY: The present study investigated the in vitro effect of IL-17 on progesterone and human chorionic gonadotropin (hCG) secretion by JEG-3 cells. Both hormones were quantified using enzyme-linked immunosorbent assays. RESULTS: The results showed that IL-17 significantly increased progesterone secretion at 6 (P < 0.001) and 24 (P < 0.01) hr, while this cytokine had no effect on hCG secretion. CONCLUSION: Interleukin-17 may regulate the function of JEG-3 cells through increased progesterone secretion.
Asunto(s)
Coriocarcinoma/metabolismo , Gonadotropina Coriónica/metabolismo , Interleucina-17/metabolismo , Progesterona/metabolismo , Receptores de Interleucina-17/metabolismo , Línea Celular Tumoral , Coriocarcinoma/inmunología , Gonadotropina Coriónica/genética , Gonadotropina Coriónica/inmunología , Ensayo de Inmunoadsorción Enzimática , Femenino , Humanos , Interleucina-17/inmunología , Interleucina-17/farmacología , Embarazo , Progesterona/genética , Progesterona/inmunología , Receptores de Interleucina-17/inmunologíaRESUMEN
PROBLEM: As there has been a study in mice showing the expression of IL-17 by decidual cells and the status of IL-17 receptor expression in human pregnancy is not known, we hypothesized that IL-17 may regulate human trophoblast proliferation and invasion. METHOD OF STUDY: JEG-3 cell line was used as a model for human trophoblast. Immunohistochemitry and reverse transcriptase polymerase chain reaction techniques were used to identify IL-17 receptor protein and mRNA, respectively. The effects of IL-17 on JEG-3 cell proliferation and invasion were tested using the BrdU incorporation and the Matrigel invasion assays, respectively. RESULTS: IL-17 increased the invasive capacity of JEG-3 cells but had no effect on the proliferation and multinucleated formation of JEG-3 cells. CONCLUSION: In this JEG-3 cell model of human trophoblast, the IL-17R and IL-17 may have a regulatory role in trophoblast invasion.