Asunto(s)
Infecciones Bacterianas/diagnóstico , Virosis/diagnóstico , Ensayo de Inmunoadsorción Enzimática/métodos , Ensayo de Inmunoadsorción Enzimática/tendencias , Hospitales/normas , Hospitales/tendencias , Humanos , Pruebas Inmunológicas , Laboratorios de Hospital , Reacción en Cadena de la Polimerasa/métodos , Reacción en Cadena de la Polimerasa/normas , Reacción en Cadena de la Polimerasa/tendencias , Prueba de Radioinmunoadsorción/métodos , Prueba de Radioinmunoadsorción/normas , Prueba de Radioinmunoadsorción/tendenciasRESUMEN
Comparative analysis of association with psoriasis before and after treatment of 53K/H2A and 43K/H2A leukocyte protein markers and parameters of leukocyte population (18 indexes) including concentration of peripheral blood lymphocyte subpopulations as markers of the immune status revealed advantages of the former method of patient monitoring for the evaluation of treatment efficacy. The method showed 100% sensitivity and correlation with the dynamics of clinical symptoms. A significant correlation of 53K/H2A parameter with blood content of CD3+, CD4+, CD8+, and CD72+ lymphocytes was established.
Asunto(s)
Genoma , Leucocitos/metabolismo , Psoriasis/genética , Psoriasis/inmunología , Adolescente , Adulto , Antígenos CD/biosíntesis , Antígenos de Diferenciación de Linfocitos B/biosíntesis , Complejo CD3/biosíntesis , Antígenos CD4/biosíntesis , Antígenos CD8/biosíntesis , Niño , Femenino , Humanos , Linfocitos/metabolismo , Masculino , Persona de Mediana Edad , Psoriasis/sangreRESUMEN
The content of 53K/H2A and 43K/H2A proteins in leukocytes of patients with psoriasis, newborns, and healthy donors was studied. In all patients one or both parameters differed from normal. Mean values and distribution of 53K/H2A in healthy siblings of psoriatic proband significantly differed from those in newborns and donors. It was concluded that 53K/H2A is a marker of pathological changes in the genome responsible for predisposition to psoriasis. This marker can be revealed in peripheral blood leukocytes before skin manifestations of the disease.