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BACKGROUND: Alzheimer's disease (AD) is a progressive neurodegenerative disease that can cause dementia. We aim to screen out the hub genes involved in AD based on microarray datasets. METHODS: Gene expression profiles GSE5281 and GSE28146 were retrieved from Gene Expression Omnibus database to acquire differentially expressed genes (DEGs). Gene Ontology and pathway enrichment were conducted using DAVID online tool. The STRING database and Cytoscape tools were employed to analyze protein-protein interactions and identify hub genes. The predictive value of hub genes was assessed by principal component analysis and receiver operating characteristic curves. AD mice model was constructed, and histology was then observed by hematoxylin-eosin staining. Gene expression levels were finally determined by real-time quantitative PCR. RESULTS: We obtained 197 overlapping DEGs from GSE5281 and GSE28146 datasets. After constructing protein-protein interaction network, three highly interconnected clusters were identified and 6 hub genes (RBL1, BUB1, HDAC7, KAT5, SIRT2, and ITGB1) were selected. The hub genes could be used as basis to predict AD. Histological abnormalities of brain were observed, suggesting successful AD model was constructed. Compared with the control group, the mRNA expression levels of RBL1, BUB1, HDAC7, KAT5 and SIRT2 were significantly increased, while the mRNA expression level of ITGB1 was significantly decreased in AD groups. CONCLUSION: RBL1, BUB1, HDAC7, KAT5, SIRT2 and ITGB1 are promising gene signatures for diagnosis and therapy of AD.
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Enfermedad de Alzheimer , Enfermedades Neurodegenerativas , Animales , Ratones , Sirtuina 2/genética , Perfilación de la Expresión Génica , Enfermedad de Alzheimer/diagnóstico , Enfermedad de Alzheimer/genética , Mapas de Interacción de Proteínas/genética , Biología Computacional , ARN Mensajero , Redes Reguladoras de GenesRESUMEN
AIM: To explore the predictive values of serum biochemical markers and apparent diffusion coefficient (ADC) on delayed encephalopathy after acute carbon monoxide poisoning (DEACMP). MATERIAL AND METHODS: Seventy-nine patients were divided into two groups based on the onset of DEACMP 60 days after with acute carbon monoxide poisoning. The baseline data of ACMP patients were collected. Serum levels of cardiac troponin I (cTNI), lactic acid (Lac), carboxyhemoglobin (COHb), creatine kinase (CK), creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), C-reactive protein (CRP), and neuron-specific enolase (NSE) were measured within 24 hours of ACMP onset. The ADC of globus pallidum, centrum semiovale, and periventricular white matter were recorded by diffusion-weighted magnetic resonance imaging (DW-MRI) within 48 hours of admission. Logistic regression analysis was performed to determine the risk factors for DEACMP. RESULTS: The incidence of coma and duration of poisoning in the DEACMP group were significantly higher than those in the non-DEACMP group. The levels of Lac, CK, CK-MB, LDH, CRP and NSE in the DEACMP group were higher than those in the non- DEACMP group. The ADC value of globus pallidus in the DEACMP group was significantly higher than that in non-DEACMP group. The duration of poisoning, coma degree, CK, LDH, NES, and CRP were closely associated with the occurrence of DEACMP. CONCLUSION: The duration of poisoning, coma degree, and serum levels of CK, LDH, CRP, and NSE were independent risk factors for DEACMP.
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Encefalopatías , Intoxicación por Monóxido de Carbono , Sustancia Blanca , Biomarcadores , Encefalopatías/diagnóstico por imagen , Encefalopatías/etiología , Intoxicación por Monóxido de Carbono/complicaciones , Imagen de Difusión por Resonancia Magnética , HumanosRESUMEN
AIM: The study aimed to evaluate the effect of concurrent computed tomography (CT)-guided percutaneous interstitial implantation of iodine-125 (125I) seeds and chemotherapy on cervical lymph nodes metastasis. METHODS: The prospective randomized study included 82 cases with cervical lymph nodes metastasis who were admitted to our hospital from January 2010 to June 2012. All the subjects were randomly divided into the concurrent 125I implantation and chemotherapy group (n = 48) and chemotherapy-only group (n = 34) according to the treatment strategy. The concurrent 125I implantation and chemotherapy group was treated with CT-guided 125I seeds implantation and routine chemotherapy. The routine chemotherapy included paclitaxel and cisplatin. Patients were followed up for 6 months. RESULTS: In the concurrent 125I implantation and chemotherapy group, overall response rate (complete response [CR] + partial response [PR]) was 82.61% and 85.51% at 2 and 6 months posttreatment, respectively. The longest diameter of CR and PR lymph nodes was markedly decreased after treatment (P < 0.05). In the chemotherapy-only group, overall response rate was 22.45% and 10.20% at 2 and 6 months posttreatment, respectively. The number of patients with moderate to severe pain was much less in concurrent 125I implantation and chemotherapy group than that of chemotherapy-only group (4.17% vs. 17.64%; P < 0.05) at 6-month posttreatment. No treatment-related death or severe complication was reported in the two groups. CONCLUSION: Concurrent CT-guided 125I seeds implantation and chemotherapy is superior to routine chemotherapy in efficacy, safety, and pain relief in patients with cervical lymph nodes metastasis.
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Antineoplásicos/uso terapéutico , Braquiterapia/métodos , Radioisótopos de Yodo/administración & dosificación , Ganglios Linfáticos/patología , Metástasis Linfática/radioterapia , Radioterapia Guiada por Imagen/métodos , Anciano , Quimioradioterapia/métodos , Femenino , Humanos , Ganglios Linfáticos/diagnóstico por imagen , Ganglios Linfáticos/efectos de la radiación , Metástasis Linfática/diagnóstico por imagen , Masculino , Persona de Mediana Edad , Cuello , Estudios Prospectivos , Tomografía Computarizada por Rayos X , Resultado del TratamientoRESUMEN
OBJECTIVE: To investigate the carotid atherosclerotic plaque features in patients with acute ischemic stroke. METHODS: A total of 288 patients meeting the included criteria were enrolled and divided into an ulcerated plaque group (n = 139) and a nonulcerated plaque group (n = 149). Patients in the ulcerated plaque group were further subdivided into <50% and ≥50% stenosis groups. Carotid plaque component characteristics including luminal stenosis, carotid plaque volume, hypoechoic plaque volume, and hyperechoic plaque volume were analyzed by color Doppler ultrasound measurement. Associations between ulcerated plaque and carotid plaque features were also evaluated. The relationships among the levels of MMP-9, hs-CRP, and carotid stenosis rate were detected by enzyme-linked immunosorbent assay. RESULTS: The plaque volume, hypoechoic plaque volume, and luminal stenosis in the ulcerated plaque group were higher than that of the nonulcerated plaque group (P < 0.05). Ulcerated plaque was positively associated with luminal stenosis, plaque volume, and hypoechoic plaque volume after adjusting for sex and age. The result remained similar after adjusting for age, sex, and carotid luminal stenosis. The levels of MMP-9 and hs-CRP in the ulcerated plaque group were significantly higher than those of the nonulcerated plaque group (P < 0.01). For the ulcerated plaque group, the higher the carotid stenosis rate, the higher the level of MMP-9 and hs-CRP. CONCLUSIONS: Higher carotid atherosclerosis plaque volume, hypoechoic plaque volume, and luminal carotid stenosis may be symptoms of ulcerated plaque. Increased MMP-9 and hs-CRP levels could be used as adjunctive therapies of carotid stenosis at the molecular level.