RESUMEN
Corynebacterium glutamicum has a branched respiratory chain: one of the branches is cytochrome bcc complex and cytochrome aa3-type cytochrome c oxidase, and the other is cytochrome bd-type menaquinol oxidase. The factors that influence the expression patterns of these respiratory enzymes remain unclear. To investigate the expressional control mechanism of the enzymes, we have previously constructed a promoter assay system utilizing enhanced green fluorescence protein. Here, we monitored respiratory enzymes' expression by using this system during growth in various culture media, with and without Cu(2+) ion supplementation. The promoter activities of cytochrome aa3 oxidase in the early stationary phase in the media supplemented with Cu(2+) ion at 40 or 400 µM were significantly increased 1.49-fold or 1.99-fold, respectively, as compared to the control. Moreover, the H(+)/O ratio, or the proton-pumping activity of the cells, increased about 1.6 times by the Cu(2+) supplementation. These facts indicate that copper ions can switch the branches.
Asunto(s)
Cobre/farmacología , Corynebacterium glutamicum/efectos de los fármacos , Corynebacterium glutamicum/enzimología , Medios de Cultivo/química , Regulación Bacteriana de la Expresión Génica/efectos de los fármacos , Transporte Biológico/efectos de los fármacos , Membrana Celular/efectos de los fármacos , Membrana Celular/metabolismo , Cobre/análisis , Corynebacterium glutamicum/genética , Corynebacterium glutamicum/crecimiento & desarrollo , Transporte de Electrón/efectos de los fármacos , Hemo/metabolismo , Oxígeno/metabolismo , Regiones Promotoras Genéticas/genética , ProtonesRESUMEN
A few reports suggest differences in injury outcomes between cadaver tests and real-world accidents under almost similar conditions. This study hypothesized that muscle activity could primarily cause the differences, and then developed a human body finite element (FE) model with individual muscles. Each muscle was modeled as a hybrid model of bar elements with active properties and solid elements with passive properties. The model without muscle activation was firstly validated against five series of cadaver test data on impact responses in the anterior-posterior direction. The model with muscle activation levels estimated based on electromyography (EMG) data was secondly validated against four series of volunteer test data on bracing effects for stiffness and thickness of an upper arm muscle, and braced driver's responses under a static environment and a brake deceleration. A muscle controller using reinforcement learning (RL), which is a mathematical model of learning process in the basal ganglia associated with human postural controls, were newly proposed to estimate muscle activity in various occupant conditions including inattentive and attentive conditions. Control of individual muscles predicted by RL reproduced more human like head-neck motions than conventional control of two groups of agonist and antagonist muscles. The model and the controller demonstrated that head-neck motions of an occupant under an impact deceleration of frontal crash were different in between a bracing condition with maximal braking force and an occupant condition predicted by RL. The model and the controller have the potential to investigate muscular effects in various occupant conditions during frontal crashes.
Asunto(s)
Accidentes de Tránsito , Diseño de Equipo/métodos , Análisis de Elementos Finitos , Modelos Anatómicos , Modelos Biológicos , Músculo Esquelético/fisiología , Desaceleración , Electromiografía , Cabeza/fisiología , Humanos , Masculino , Cuello/fisiología , Postura/fisiología , Reproducibilidad de los ResultadosRESUMEN
We previously developed an analyzer able to detect hydrogen concentrations of less than 50 cm3/1000 m3. The analyzer uses a carrier gas purifier and a low temperature separation column to remove impurities preventing measurement of low concentrations from the carrier and sample gases. It uses a trace reduction detector with a mercuric oxide bed to detect the concentration of hydrogen based on the reduction reaction of mercuric oxide with hydrogen. We have now evaluated the performance of the analyzer by carrying out a series of tests that measured the spectrum peak and the retention time. We used three sample gases with hydrogen concentrations of 5, 20, and 50 cm3/1000 m3 in nitrogen dilution gas. The measured peak was stable (it was within a relative standard deviation of less than 10%), and there was a linear relationship between the peak and hydrogen concentration. However, the retention time gradually shortened as the measurements were repeated. The shortening was reduced by warming the low temperature separation column used in the analyzer; it was not observed when we used a hydrogen sample gas diluted by helium instead of nitrogen. Using nitrogen as a dilution gas apparently shortens the retention time. We thus added an MS-5A separation column and a thermal conductivity detector. The nitrogen and hydrogen in the sample/carrier gas are separated, and the nitrogen is efficiently removed by switching the pass line to a release line after the hydrogen has been sent to the low temperature separation column. An analyzer using this "after-cut method" was able to stably measure infinitesimal hydrogen concentrations and was not affected by nitrogen in the sample gas.
Asunto(s)
Hidrógeno/análisis , Cromatografía de Gases , Espectrofotometría AtómicaRESUMEN
The following case report describes the buccal and coronal bone augmentation around an irretrievable tooth using forced eruption and buccal root torque for immediate nonsubmerged implant placement. A mandibular left second premolar with degree 2 mobility, severe buccal bone resorption, and interproximal angular bony defects was subjected to forced eruption and buccal root torque. Five months after this process, the tooth was displaced 15 mm coronally and the root apex faced buccally. Buccal and coronal bone augmentation and soft tissue enlargement were evident at reentry surgery. This technique enabled proper implant placement in a situation where the bone was compromised.
Asunto(s)
Aumento de la Cresta Alveolar/métodos , Implantación Dental Endoósea/métodos , Técnicas de Movimiento Dental/métodos , Diente Premolar , Regeneración Ósea , Prótesis Dental de Soporte Implantado , Dentadura Parcial Inmediata , Femenino , Humanos , Mandíbula , Persona de Mediana Edad , Erupción Dental , TorqueRESUMEN
We describe a case of gingival recession in which root coverage and coronal bone regrowth were achieved after treatment with a connective tissue-bone graft and enamel matrix derivative. The connective tissue-bone graft was harvested from a maxillary edentulous area and then curved to fit the root surfaces of the maxillary left central and lateral incisors. Enamel matrix derivative was applied to the root surfaces, and the connective tissue-bone graft was fixed to the interdental bone by a titanium screw. Six months later, the exposed roots were covered with thick gingiva, and coronal regrowth of thick bone was evident at reentry surgery. This technique is useful for esthetic restoration placement with an intracrevicular margin on teeth with a thin, receding gingiva.