RESUMEN
The objective of the study was to realize whether specific signatures for high-risk human papilloma virus (HPV) infection exist in the cytologic specimens with ASC-US judgement or not. The materials are 132 cytologic specimens with the diagnosis of ASC-US, including 56 cases with positive and 76 cases with negative HPV infection. Cytological findings are compared between two groups. Immature squamous metaplastic cells with nuclear atypia, SFT/IMT dyskaryotic cells, atypical parakeratosis, smudgy nuclei and multinucleated cells are the signature of high-risk HPV infection, whereas in the HPV(-) group immature metaplastic cells without atypia, moderately mature metaplastic cells without nuclear atypia and atrophic background are more popular. Instead, there are no differences on SFT/IMT background, microorganism infection and koilocytosis with or without nuclear atypia in both groups. The specific findings to confirm high-risk HPV infection are realized and the present results will contribute to decrease an unnecessary ASC-US judgement.
Asunto(s)
Células Escamosas Atípicas del Cuello del Útero , Técnicas Citológicas , Infecciones por Papillomavirus , Femenino , Humanos , RiesgoRESUMEN
OBJECTIVE: To evaluate the usefulness of a new liquid-based cytological procedure in a population screening program for cervix cancer. SUBJECTS AND METHODS: Subjects were 1,000 women who underwent primary screening at the Kanagawa Health Service Association. The cytological specimens obtained by either cotton stick and Cytobrush® or Cervex-Brush® were processed using the Thinlayer Advanced Cytology Assay System (TACAS™), following the manufacturer's instructions. RESULTS: (1) Cells were evenly distributed on specimens and stained evenly; (2) shrinkage of cells was 5% based on measurement of the nuclear diameters of granulocytes in comparison with those of the conventional procedure; (3) incidences of cells that occupied the whole area, 1/20≤, 1/4≤, 1/4> of the observation fields were 58.8, 26.2, 12.0 and 3.0%, respectively; (4) number of the squamous cells in cases with 1/4> was <5,000, in which specimen cells were correctly obtained from the squamocolumnar junction except in 3 cases (0.3%); (5) bleeding at cellular sampling was 5%, but did not disturb cell analysis; (6) inflammation caused by organisms was easily diagnosed; (7) detection rate of abnormal cytology was 4.3%, including ASC-US in 2.8% and ASC-H in 0.1%. CONCLUSION: TACAS is a feasible and useful cytological procedure.