RESUMEN

Recombinant adenoviral vectors have emerged as an attractive system for veterinary vaccines development. However, for poultry vaccination a very important criterion for an ideal vaccine is its low cost. The objective of this study was to test the ability of chicken CD154 to enhance the immunogenicity of an adenoviral vector-based vaccine against avian influenza virus in order to reduce the amount of antigen required to induce an effective immune response in avian. Chickens were vaccinated with three different doses of adenoviral vectors encoding either HA (AdHA), or HA fused to extracellular domain chicken's CD154 (AdHACD). Hemagglutination inhibition (HI) assay and relative quantification of IFN-γ showed that the adenoviral vector encoding for the chimeric antigen is able to elicit an improved humoral and cellular immune response, which demonstrated that CD154 can be used as a molecular adjuvant allowing to reduce in about 50-fold the amount of adenoviral vector vaccine required to induce an effective immune response.

Asunto(s)

Ligando de CD40/inmunología , Inmunidad Celular/inmunología , Inmunidad Humoral/inmunología , Proteínas Recombinantes de Fusión/inmunología , Vacunas Virales/inmunología , Adenoviridae/genética , Animales , Anticuerpos Antivirales/sangre , Ligando de CD40/genética , Línea Celular , Pollos , Orden Génico , Vectores Genéticos/genética , Interferón gamma/inmunología , Leucocitos Mononucleares/inmunología , Ratones , Proteínas Recombinantes de Fusión/genética , Vacunas Virales/genética