RESUMEN
In our screening program for new biologically active secondary metabolites, nine new polycyclic polyprenyled acylphloroglucinols, hyperscabins D-L, together with three known compounds, were obtained from the aerial parts of Hypericum scabrum. The chemical structures of 1-9 were characterized by extensive spectroscopic analyses, nuclear magnetic resonance calculation with DP4+ probability analysis, and the electronic circular dichroism spectra were calculated. Compound 1 was an unusual prenylated acylphloroglucinol decorated with a 5-oxaspiro [4,5] deca-1,9-dione skeleton. Compound 2 was a newly identified spirocyclic polyprenylated acylphloroglucinol possessing a rare 5,5-spiroketal segment. Compounds 3, 8, and 10 (10 µM) exhibited pronounced hepatoprotective activity against d-galactosamine-induced WB-F344 cell damage in vitro assays. All test compounds (1, 3, and 7-12) demonstrated potential inhibitory effects at 10 µM against noradrenalinet ([3H]-NE) reuptake in rat brain synaptosome.
Asunto(s)
Antidepresivos/farmacología , Hemiterpenos/farmacología , Hypericum/química , Floroglucinol/análogos & derivados , Floroglucinol/farmacología , Sustancias Protectoras/farmacología , Animales , Antidepresivos/síntesis química , Antidepresivos/aislamiento & purificación , Línea Celular , Hemiterpenos/síntesis química , Hemiterpenos/aislamiento & purificación , Inhibidores de la Captación de Neurotransmisores/síntesis química , Inhibidores de la Captación de Neurotransmisores/aislamiento & purificación , Inhibidores de la Captación de Neurotransmisores/farmacología , Norepinefrina/metabolismo , Floroglucinol/aislamiento & purificación , Componentes Aéreos de las Plantas/química , Sustancias Protectoras/síntesis química , Sustancias Protectoras/aislamiento & purificación , Ratas , Sinaptosomas/efectos de los fármacos , Sinaptosomas/metabolismoRESUMEN
Four new dianthrone glycosides, named polygonumnolides C1-C4 (1-4), were isolated from the dried roots of Polygonum multiflorum Thunb, together with two known emodin dianthrones (5-6). Their hepatotoxicities were evaluated against L-02 cell lines. Compounds 1-4 showed weak hepatotoxicity against L-02 cell lines with IC50 values of 313.05, 205.20, 294.20, and 207.35 µM, respectively.
Asunto(s)
Antracenos/aislamiento & purificación , Medicamentos Herbarios Chinos/aislamiento & purificación , Glicósidos/aislamiento & purificación , Hígado/efectos de los fármacos , Polygonum/química , Antracenos/química , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/farmacología , Emodina/química , Glicósidos/química , Humanos , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Raíces de Plantas/química , EstereoisomerismoRESUMEN
Two new prenylated phloroglucinol derivatives (1-2), and a known compound furohyperforim isomer 2 (3), were isolated from the aerial parts of Hypericum scabrum. Their structures were elucidated by various spectroscopic methods, including MS, IR, UV, and NMR.
Asunto(s)
Medicamentos Herbarios Chinos/aislamiento & purificación , Hypericum/química , Floroglucinol/aislamiento & purificación , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/farmacología , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Floroglucinol/química , Prenilación , EstereoisomerismoRESUMEN
Four new alkenes (1-4), and six known alkenes (5-12) were isolated from Murraya koenigii (L.) Spreng. Their structures were elucidated on the basis of spectroscopic analyses and references. Compounds (1-12) were evaluated for antioxidative activities. Among them, compounds 1, 2, 4, and 7 exhibited significant antioxidative activities using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay with IC50=21.4-49.5 µM. The known compounds (5-12) were isolated from this plant for the first time.
Asunto(s)
Alquenos/química , Antioxidantes/química , Murraya/química , Extractos Vegetales/química , Alquenos/aislamiento & purificación , Antioxidantes/aislamiento & purificación , Dicroismo Circular , Concentración 50 Inhibidora , Espectroscopía de Resonancia Magnética , Conformación Molecular , Murraya/metabolismo , Hojas de la Planta/química , Hojas de la Planta/metabolismoRESUMEN
Ten tirucallane-type triterpenes named boscartene A-J and a nor-tetracyclic triterpene boscartene K, together with ten known compounds were isolated from the gum resin of Boswellia carterii Birdw. Their structures and absolute configurations were elucidated by extensive spectroscopic analysis. In vitro assay, some of these compounds (10 µM) showed moderate hepatoprotective activities against d-galactosamine-induced HL-7702 cell damage.
Asunto(s)
Boswellia/química , Hepatocitos/efectos de los fármacos , Resinas de Plantas/química , Triterpenos/química , Línea Celular , Citoprotección , Galactosamina/efectos adversos , Humanos , Estructura Molecular , Triterpenos/aislamiento & purificaciónRESUMEN
Eight new cembranoids, boscartins A-H (1, 2, and 4-9), and the known incensole oxide were isolated from the gum resin of Boswellia carterii. The absolute configurations of 1, 2, 4, and incensole oxide were unequivocally resolved using single-crystal X-ray diffraction analysis with Cu Kα radiation, and the absolute configuration of 5 was resolved via electronic circular dichroism data. The antiulcerative colitis activities of the compounds were evaluated in an in vitro x-box-binding protein 1 (XBP 1) transcriptional activity assay using dual luciferase reporter detection. At 10 µM, compounds 1, 5, 6, and 7 significantly activated XBP 1 transcription with EC50 values of 0.34, 1.14, 0.88, and 0.42 µM, respectively, compared with the pGL3-basic vector control.
Asunto(s)
Antiulcerosos/aislamiento & purificación , Antiulcerosos/farmacología , Boswellia/química , Colitis/tratamiento farmacológico , Diterpenos/aislamiento & purificación , Resinas de Plantas/química , Antiulcerosos/química , Cristalografía por Rayos X , Proteínas de Unión al ADN/efectos de los fármacos , Diterpenos/química , Conformación Molecular , Estructura Molecular , Factores de Transcripción del Factor Regulador X , Factores de Transcripción/efectos de los fármacosRESUMEN
Two new benzofurans, gymnefuranols A (1) and B (2), together with six known furanolignans (3-8), were isolated from Gymnema tingens. The structures of the new compounds were elucidated by comprehensive analysis of the NMR and HR-MS data. Compounds 1, 2, 6, and 7 showed hepatoprotective activities against D-galactosamine-induced HL-7702 cell damage.
Asunto(s)
Benzofuranos/aislamiento & purificación , Benzofuranos/farmacología , Gymnema/química , Lignanos/aislamiento & purificación , Lignanos/farmacología , Hígado/efectos de los fármacos , Benzofuranos/química , Galactosamina/farmacología , Lignanos/química , Estructura Molecular , Resonancia Magnética Nuclear BiomolecularRESUMEN
Natural product Hypericum perforatum L. has been used in folk medicine to improve mental performance. However, the effect of H. perforatum L. on metabolism is still unknown. In order to test whether H. perforatum L. extract (EHP) has an effect on metabolic syndrome, we treated diet induced obese (DIO) C57BL/6J mice with the extract. The chemical characters of EHP were investigated with thin-layer chromatography, ultraviolet, high-performance liquid chromatography (HPLC), and HPLC-mass spectrometry fingerprint analysis. Oral glucose tolerance test (OGTT), insulin tolerance test (ITT), and the glucose infusion rate (GIR) in hyperinsulinemic-euglycemic clamp test were performed to evaluate the glucose metabolism and insulin sensitivity. Skeletal muscle was examined for lipid metabolism. The results suggest that EHP can significantly improve the glucose and lipid metabolism in DIO mice. In vitro, EHP inhibited the catalytic activity of recombinant human protein tyrosine phosphatase 1B (PTP1B) and reduced the protein and mRNA levels of PTP1B in the skeletal muscle. Moreover, expressions of genes related to fatty acid uptake and oxidation were changed by EHP in the skeletal muscle. These results suggest that EHP may improve insulin resistance and lipid metabolism in DIO mice.
Asunto(s)
Hypericum/química , Resistencia a la Insulina , Metabolismo de los Lípidos/efectos de los fármacos , Síndrome Metabólico/metabolismo , Extractos Vegetales/farmacología , Animales , Dieta Alta en Grasa/efectos adversos , Ácidos Grasos/metabolismo , Glucosa/metabolismo , Prueba de Tolerancia a la Glucosa , Humanos , Masculino , Síndrome Metabólico/tratamiento farmacológico , Ratones , Ratones Endogámicos C57BL , Ratones Obesos , Músculo Esquelético/metabolismo , Obesidad/tratamiento farmacológico , Obesidad/metabolismo , Componentes Aéreos de las Plantas/química , Proteína Tirosina Fosfatasa no Receptora Tipo 1 , Proteínas Recombinantes/metabolismoRESUMEN
In present study, the anthocyanin composition and content of the fruit of B. heteropoda Schrenk were determined for the first time. The total anthocyanins were extracted from the fruit of B. heteropoda Schrenk using 0.5% HCl in 80% methanol and were then purified using an AB-8 macroporous resin column. The purified anthocyanin extract (PAE) was evaluated by high-performance liquid chromatography with a diode array detector (HPLC-DAD) and HPLC-high resolution-electrospray ionization-mass spectrometry (HPLC-HR-ESI-MS) under the same experimental conditions. The results revealed the presence of seven different anthocyanins. The major anthocyanins purified by preparative HPLC were confirmed to be delphinidin-3-O-glucopyranoside (30.3%), cyanidin-3-O-glucopyranoside (33.5%), petunidin-3-Ο-glucopyranoside (10.5%), peonidin-3-O-glucopyranoside (8.5%) and malvidin-3-O-glucopyranoside (13.8%) using HPLC-HR-ESI-MS and NMR spectroscopy. The total anthocyanin content was 2036.6 ± 2.2 mg/100 g of the fresh weight of B. heteropoda Schrenk fruit. In terms of its total reducing capacity assay, DPPH radical-scavenging activity assay, ferric-reducing antioxidant power (FRAP) assay and ABTS radical cation-scavenging activity assay, the PAE also showed potent antioxidant activity. The results are valuable for illuminating anthocyanins composition of B. heteropoda Schrenk and for further utilising them as a promising anthocyanin pigment source. This research enriched the chemical information of B. heteropoda Schrenk.
Asunto(s)
Antocianinas/química , Antioxidantes/química , Berberis/química , Frutas/química , Cromatografía Líquida de Alta Presión/métodos , Glucósidos/química , Espectrometría de Masa por Ionización de Electrospray/métodosRESUMEN
To study the chemical constituents of Cymbopogon citratus, isolation and purification of constituents were carried out on silica gel, Sephadex LH-20 and prepatative HPLC. The structures of the compounds were identified by physicchemical properties and spectral data analysis. Eight compounds were isolated and identified as 3beta-methoxy lanosta-9(11)-en-27-ol (1), 3beta-hydroxylanosta-9 (11)-en (2), (24S) -3beta-methoxylanosta-9(11), 25-dien-24-ol (3), 8-hydroxyl-neo-menthol (4), (2E)-3,7-dimethyl-2,7-octadiene-1, 6-diol (5), (+)-citronellol (6), 7-hydroxymenthol (7) and ethyl nonadecanoate(8). Compounds 1 is a new one. Compounds 2-3 are obtained from C. citratus for the first time.
Asunto(s)
Cymbopogon/química , Medicamentos Herbarios Chinos/química , Triterpenos/química , Estructura Molecular , Espectrometría de Masa por Ionización de ElectrosprayRESUMEN
Allium cepa L. is one of the most widely cultivated and used plants. In addition to its bulb (onion), which is used as food in many cultures, the seeds of A. cepa L. are used as a traditional herbal medicine by the Uygur nationality in China to treat diarrhea and promote blood flow. In a bioactivity-screening, the ethanol extract of seeds of A. cepa L. showed inhibitory effects on protein tyrosine phosphatase 1B (PTP1B) enzyme, with 81.1% inhibition. Phytochemical investigation of the ethanol extract of red onion (Allium cepa L.) seeds led to the isolation of eight new furostanol saponins, named ceparosides E-L (1-8). Their structures were established using 1D and 2D NMR spectroscopy, mass spectrometry and chemical methods. Compounds 1-8 were screened for inhibitory effects on the PTP1B enzyme and cytotoxic activity against five human cells, including HCT-8, Bel-7402, BGC-823, A549 and A2780, but all were found to be inactive.
Asunto(s)
Cebollas/química , Fitosteroles/química , Saponinas/química , Semillas/química , Glucosa-6-Fosfato Isomerasa , Humanos , Estructura Molecular , Fitosteroles/aislamiento & purificación , Extractos Vegetales/química , Proteína Tirosina Fosfatasa no Receptora Tipo 1/antagonistas & inhibidores , Saponinas/aislamiento & purificaciónRESUMEN
Two new polyprenylated acylphloroglucinols, (1S,32R,5S,6R,7R)-6-((R)-3,4-di-hydroxy-4-methylpentyl)-2-(2-hydroxypropan-2-yl)-7-isobutyryl-6-methyl-5,9-bis(3-methylbut-2-en-1-yl)-4,5,6,7-tetrahydro-2H-32,7-methanocycloocta[b]furan-8,10(3H)-dione (1) and (4R,5R,7R)-4-((R)-3,4-dihydroxy-4-methylpentyl)-2,2,4-trimethyl-5,7-bis(3-methyl-but-2-en-1-yl)-7-(5-methylhex-4-enoyl)-4,5,6,7-tetrahydrobenzofuran-3(2H)-one (2) were isolated from Hypericum scabrum. The structures were elucidated by means of spectroscopic methods, including MS, IR, NMR, OR, and CD.
Asunto(s)
Medicamentos Herbarios Chinos/aislamiento & purificación , Hypericum/química , Floroglucinol/análogos & derivados , Medicamentos Herbarios Chinos/química , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Floroglucinol/química , EstereoisomerismoRESUMEN
Two new cucurbitane triterpenoids 1 and 2 were isolated, together with six known compounds, from the seeds of Momordica charantia L. The structures of new compounds were determined to be 3-O-{[ß-d-galactopyranosyl(1 â 6)]-O-ß-d-galactopyranosyl}-23(R), 24(R), 25-trihydroxycucur-bit-5-ene (1), 3-O-[ß-d-galactopyranosyl]-25-O-ß-d-galactopyranosyl-7(R), 22(S), 23(R), 24(R), 25-pentahydroxycucurbit-5-ene (2), respectively. Their structures were elucidated by the combination of mass spectrometry, one- and two-dimensional NMR experiments and chemical reactions.
Asunto(s)
Glicósidos/aislamiento & purificación , Momordica charantia/química , Triterpenos/aislamiento & purificación , Frutas/química , Glicósidos/química , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Semillas/química , Estereoisomerismo , Triterpenos/químicaRESUMEN
A new dimeric phthalide, chaxiongnolide A (1), and a new natural product, chaxiongnolide B (2), together with a known compound Z-ligustilide (3), were isolated from the rhizome of Ligusticum sinense Oliv cv. Chaxiong. Their structures were elucidated by various spectroscopic methods and confirmed by X-ray crystallographic analysis.
Asunto(s)
Benzofuranos/aislamiento & purificación , Medicamentos Herbarios Chinos/aislamiento & purificación , Ligusticum/química , 4-Butirolactona/análogos & derivados , 4-Butirolactona/química , 4-Butirolactona/aislamiento & purificación , Benzofuranos/química , Cristalografía por Rayos X , Medicamentos Herbarios Chinos/química , Conformación Molecular , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Rizoma/químicaRESUMEN
Three new sesquiterpenes (1-3) and two new rutinosides (4 and 5) along with 17 known compounds (6-22) were isolated from the leaves of Murraya koenigii (L.) Spreng. The new compounds were elucidated as (3R,5S,6R)-3,5,6-trihydroxy-1,1,5-trimethylcyclohexyl-8-butyn-9-one (1), (8E,9R)-ethyl-7-(3S,5R,6S)-3,6-dihydroxy-1,1,5-trimethylcyclohexyl-9-hydroxybut-8-enoate (2), (3R)-3-O-ß-D-glucoside-6'-D-apiose-ß-ionone (3), 4-O-ß-D-rutinosyl-3-methoxyphenyl-1-propanone (4), and 1-O-ß-D-rutinosyl-2(R)-ethyl-1-pentanol (5) based on their spectroscopic data. Compounds 1, 4, 5, 18, and 21 (10 µM) exhibited moderate hepatoprotective activities.
Asunto(s)
Hepatocitos/efectos de los fármacos , Murraya/química , Extractos Vegetales/química , Sustancias Protectoras/química , Rutina/química , Sesquiterpenos/química , Línea Celular , Humanos , Estructura Molecular , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/farmacología , Hojas de la Planta/química , Sustancias Protectoras/aislamiento & purificación , Sustancias Protectoras/farmacología , Rutina/aislamiento & purificación , Rutina/farmacología , Sesquiterpenos/aislamiento & purificación , Sesquiterpenos/farmacologíaRESUMEN
Two new bidesmoside triterpenoid saponins which were identifed as 28-O-ß-D-xylopyranosyl(1â3)-ß-D-xylopyranosyl(1â4)-α-L-rhamnopyranosyl(1â2)-[α-L-rhamno-pyranosyl(1â3)]-ß-D-fucopyranosyl gypsogenin 3-O-ß-D-glucopyranosyl (1â2)-ß-D-glucopyranosiduronic acid (C1) and 28-O-ß-D-xylopyranosyl(1â4)-α-L-rhamnopyranosyl (1â2)-[α-L-rhamnopyranosyl(1â3)]-ß-D-fucopyranosyl gypsogenin 3-O-ß-D-gluco-pyranosyl(1â2)-ß-D-glucopyranosiduronic acid (C2) were isolated together with two known compounds from the seeds of Momordica charantia L. Their structures were elucidated by the combination of mass spectrometry (MS), one and two-dimensional NMR experiments and chemical reactions.
Asunto(s)
Momordica charantia/química , Saponinas/química , Semillas/química , Triterpenos/química , Espectroscopía de Resonancia Magnética , Espectrometría de Masas , Estructura Molecular , Saponinas/aislamiento & purificación , Triterpenos/aislamiento & purificaciónRESUMEN
Two new oligostilbenes, parthenocissins M (1) and N (2), together with two known compounds, miyabenol C (3) and ϵ-viniferin (4), were isolated from the stem of Parthenocissus quinquefolia. Their structures were elucidated by means of NMR, UV, IR, and MS data.
Asunto(s)
Medicamentos Herbarios Chinos/aislamiento & purificación , Estilbenos/aislamiento & purificación , Vitaceae/química , Benzofuranos/aislamiento & purificación , Medicamentos Herbarios Chinos/química , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Tallos de la Planta/química , Estilbenos/químicaRESUMEN
Chemical examination of the exuded gum resin of Boswellia carterii resulted in the isolation of nine new prenylaromadendrane-type diterpenes, boscartols A-I (1-9). The structures of these compounds were established by extensive 1D and 2D NMR spectroscopic analyses, mass spectrometric data, and circular dichroism spectra. Compounds 1-3, 5, 6, 8, and 9 (10 µM) showed moderate hepatoprotective activity against d-galactosamine-induced HL-7702 cell damage.
Asunto(s)
Boswellia/química , Diterpenos/aislamiento & purificación , Diterpenos/farmacología , Hígado/efectos de los fármacos , Resinas de Plantas/farmacología , Triterpenos/aislamiento & purificación , Triterpenos/farmacología , Diterpenos/química , Galactosamina/farmacología , Encía/efectos de los fármacos , Humanos , Factores Inmunológicos , Hígado/metabolismo , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Resinas de Plantas/química , Triterpenos/químicaRESUMEN
Six new phenolic diglycosides, named gymnetinosides A-F (1-6), were isolated from the ethanolic extract of Gymnema tingens, together with three known diglycosides, sequinoside K (7), khaephuoside B (8), and albibrissinoside A (9). The structures of the new compounds were determined by spectroscopic techniques including 1D-, 2D NMR, mass spectroscopy, and circular dichroism. Compounds 1, 5, and 6 showed hepatoprotective activities against D-galactosamine-induced HL-7702 cell damage.
Asunto(s)
Gymnema/química , Sustancias Protectoras/química , Sustancias Protectoras/farmacología , Línea Celular/efectos de los fármacos , Dicroismo Circular , Evaluación Preclínica de Medicamentos/métodos , Galactosamina/toxicidad , Glicósidos/química , Glicósidos/farmacología , Humanos , Espectroscopía de Resonancia Magnética , Estructura Molecular , Fenoles/química , Extractos Vegetales/análisis , Extractos Vegetales/química , Extractos Vegetales/farmacologíaRESUMEN
A chemical investigation on the aerial parts of Hypericum scabrum L. resulted in the isolation of three new phloroglucinol derivatives, hyperscabrins A (1), B (2), and C (3), together with one known compound, (2R,3R,4S,6R)-3-methyl-4,6-di(3-methyl-2-butenyl)-2-(2-methyl-1-oxopropyl)-3-(4-methyl-3-pentenyl)-cyclohexanone (4). The structures were elucidated by means of spectroscopic methods, including MS, IR, 1D NMR, and 2D NMR, and the absolute configurations of chiral centers in these phloroglucinol derivatives were determined for the first time by studying their circular dichroism spectra.