RESUMEN
We cloned a gene, abeM, for a multidrug efflux pump from Acinetobacter baumannii using Escherichia coli as the host. Sequence analysis revealed that AbeM is a member of the MATE family of pumps. AbeM was found to be an H(+)-coupled multidrug efflux pump and a unique member of the MATE family.
Asunto(s)
Acinetobacter baumannii/efectos de los fármacos , Antiportadores/metabolismo , Resistencia a Múltiples Medicamentos/genética , Bombas de Protones/metabolismo , Acinetobacter baumannii/genética , Acinetobacter baumannii/metabolismo , Antibacterianos/metabolismo , Antibacterianos/farmacología , Antiportadores/genética , Secuencia de Bases , Transporte Biológico , Clonación Molecular , ADN Bacteriano , Farmacorresistencia Bacteriana , Escherichia coli/genética , Escherichia coli/crecimiento & desarrollo , Genes Bacterianos , Cinética , Pruebas de Sensibilidad Microbiana , Bombas de Protones/genética , Análisis de Secuencia de ADN , Especificidad por SustratoRESUMEN
We cloned a gene responsible for norfloxacin resistance from the chromosomal DNA of Haemophilus influenzae Rd, and designated the gene as hmrM. HmrM showed sequence similarity with NorM of Vibrio parahaemolyticus and YdhE of Escherichia coli and others that belong to the MATE family multidrug efflux pumps. The recombinant plasmid carrying the hmrM gene conferred elevated resistance not only to norfloxacin but also to acriflavine, 4 ', 6-diamidino-2-phenylindole, doxorubicin, ethidium bromide, tetraphenylphosphonium chloride, Hoechst 33342, daunomycin, berberine, and sodium deoxycholate in Escherichia coli KAM32, a drug-hypersensitive strain. We observed an Na+-dependent efflux of ethidium and an ethidium-induced efflux of Na+ in E. coli KAM32 cells harboring the plasmid carrying the hmrM gene. These results indicate that HmrM is an Na+/drug antiporter-type multidrug efflux pump. A difference in substrate preference was observed between HmrM, NorM, and YdhE.