Asunto(s)
Envejecimiento , Estereotipo , Anciano , Canadá , Conocimientos, Actitudes y Práctica en Salud , Humanos , Prejuicio , Estudiantes , Estados UnidosRESUMEN
OBJECTIVE: The purpose of this study was to evaluate the role of saline solution infusion sonohysterography in clinical practice in patients with abnormal uterine bleeding. STUDY DESIGN: A prospective case-controlled study was conducted comparing two-dimensional transvaginal imaging with saline solution infusion sonohysterography, endometrial biopsies, and histologic evaluation after surgical procedures. One hundred twenty-four patients with abnormal uterine bleeding were scanned transabdominally and transvaginally. Patients with an increased endometrial thickness or a poorly defined endometrium underwent saline solution infusion sonohysterography. Sterile saline solution, 4 to 10 ml, was injected into the endometrial cavity under direct ultrasonographic visualization. Once the endometrium was expanded, the presence of polyps or myomas and the anterior and posterior endometrial thickness was assessed. RESULTS: Fifty-six patients were noted to have uterine leiomyomas. Eighteen patients had endometrial polyps. Five patients had simple endometrial hyperplasia. Two patients had atypical hyperplasia. No patients in this study had endometrial cancer. No complications occurred in this group of patients. CONCLUSION: Saline solution infusion sonohysterography is a procedure that aids the clinician in the management of abnormal uterine bleeding and may be more cost effective than traditional methods of evaluation.
Asunto(s)
Endometrio/diagnóstico por imagen , Ginecología/métodos , Obstetricia/métodos , Cloruro de Sodio , Hemorragia Uterina/diagnóstico por imagen , Adulto , Anciano , Estudios de Casos y Controles , Análisis Costo-Beneficio , Neoplasias Endometriales/complicaciones , Neoplasias Endometriales/diagnóstico por imagen , Endometrio/patología , Femenino , Humanos , Menorragia/diagnóstico , Menorragia/etiología , Menorragia/patología , Persona de Mediana Edad , Mioma/complicaciones , Mioma/diagnóstico por imagen , Pólipos/complicaciones , Pólipos/diagnóstico por imagen , Atención Primaria de Salud/economía , Atención Primaria de Salud/métodos , Atención Primaria de Salud/normas , Estudios Prospectivos , Ultrasonografía , Hemorragia Uterina/patologíaAsunto(s)
Salud , Percepción , Esfuerzo Físico , Aptitud Física , Femenino , Humanos , Masculino , Persona de Mediana EdadAsunto(s)
Actitud Frente a la Salud , Esfuerzo Físico , Anciano , Humanos , Persona de Mediana Edad , Aptitud FísicaRESUMEN
It was found that retinol at concentrations of 0.2-1.0 mg . ml-1 caused significant 51Cr release from schistosomula, while adult worms appeared unaffected. Retinol was shown, by spectrofluorimetry and fluorescence microscopy, to be absorbed into the membrane systems of both schistosomulum and adult worm, particularly when the parasites were incubated in retinol dissolved in non-ionic detergents (Tweens 20, 40 and 80). The retinol within the adult membrane could be induced to cause detectable 51Cr and 125I wheat germ agglutinin release if the adult was treated with retinol in combination with Tween 20. The effect of the combination of Tween 20 and retinol, was synergistic for the release of both isotopes. Their synergism was also observed when haemolysis of human erythrocytes was measured. Thus it is possible to greatly enhance the effect on the schistosome and the erythrocyte membrane of one membrane-active compound by presenting it in combination with another. This may have implications in chemotherapy when membrane active drugs are employed.
Asunto(s)
Polisorbatos/farmacología , Schistosoma mansoni/efectos de los fármacos , Vitamina A/farmacología , Animales , Membrana Celular/efectos de los fármacos , Membrana Celular/metabolismo , Sinergismo Farmacológico , Membrana Eritrocítica/efectos de los fármacos , Hemólisis/efectos de los fármacos , Humanos , Schistosoma mansoni/crecimiento & desarrollo , Temperatura , Vitamina A/metabolismoRESUMEN
The effects of a variety of lipophilic compounds on the young stage (schistosomulum) and adult Schistosoma mansoni have been studied by measuring the release of 51Cr and 125I-labelled wheat-germ agglutinin from labelled parasites. The compounds could be classified into three groups, one of which described reagents which affected only the schistosomulum. It is concluded that during development, changes occur in the organization of the lipid phase of the parasite membrane.
Asunto(s)
Lípidos de la Membrana/análisis , Schistosoma mansoni/análisis , Anfotericina B/farmacología , Animales , Membrana Celular/análisis , Colesterol/análisis , Polisorbatos/farmacología , Schistosoma mansoni/crecimiento & desarrollo , Schistosoma mansoni/ultraestructura , Vitamina A/farmacologíaRESUMEN
Intraperitoneal injection of cercariae into pristane (2, 6, 10, 14 tetramethyl pentadecane)-primed Balb/c mice led to greatly diminished numbers of portal and peritoneal worms compared with untreated mice. Schistosomula taken from the peritoneal cavity of pristane-primed mice carried globules of pristane on their surfaces, were contracted and were permeable to Trypan blue. Pristane globules bound also to adult worms in vitro and in vivo causing rapid damage to the surface membrane. Hydrophobic compounds other than hydrocarbons either bound without causing gross damage, or did not bind to the adult worms. 51Cr release studies showed that pristane had no effect on the permeability of human erythrocytes, while causing significant release from both schistosomula and adult worms. The binding of hydrocarbon globules to a variety of other parasites did not occur. The binding of n-[1-14C]hexadecane to adult Schistosoma mansoni was significantly decreased by extraction of the parasite with organic solvents or treatment with staphylococcal delta toxin, which interacts with phospholipids in the membrane. Possible mechanisms of damage of the parasite by the hydrocarbons are discussed.