RESUMEN
Trends of certain mastitis markers were studied in udder quarters (n = 201) showing clinical symptoms of acute mastitis. Besides the clinical examination, before the first treatment (baseline sample), and about 3 weeks later, 17 to 24 days following the last treatment (control sample) milk samples were collected for bacteriological identification of the mastitis pathogens and for the determination of certain inflammatory markers: somatic cell count (SCC), N-acetyl-beta-D-glucosaminidase (NAGase) and alpha 1-antitrypsin (ATR) activities, as well as bovine serum albumin (BSA) and chloride (Cl-) concentrations. Based upon the clinical and bacteriological status as well as the SCC recorded at the control investigations, 6 groups were established (recovered, latently infected, subclinical mastitis: bacteriologically positive and negative, as well as clinical mastitis: bacteriologically positive and negative). As compared to the baseline samples, all parameters decreased in the case of recovered udder quarters, as well as in those with abated latent infection or subclinical mastitis at the time of control examination. Comparing the control samples of the different categories, characteristic differences were found in NAGase activity, indicating the grade of cytodamaging effect of mastitis. Of the other markers, ATR and Cl- proved to be more adequate for the differentiation than BSA. It can be concluded that, in addition to SCC, first of all NAGase can be recommended for use as an inflammatory parameter in pharmacodynamic studies. Besides these two parameters, ATR and Cl- can also be chosen as a possible third marker.
Asunto(s)
Glándulas Mamarias Animales/fisiopatología , Mastitis Bovina/diagnóstico , Acetilglucosaminidasa/análisis , Animales , Biomarcadores/análisis , Bovinos , Recuento de Células/veterinaria , Cloro/análisis , Femenino , Glándulas Mamarias Animales/microbiología , Mastitis Bovina/terapia , Leche/citología , Leche/microbiología , Pronóstico , Albúmina Sérica Bovina/análisis , alfa 1-Antitripsina/análisisRESUMEN
Stability of ionized calcium (Ca2+) concentrations and pH values in equine venous samples (n = 12 in each group) stored at 4 C for 3, 9, 24, and 48 hours (blood, plasma, and serum) or for 240 hours (plasma and serum), and at -20 C for 240 hours (plasma and serum), was studied. Storage of equine blood, plasma, and serum samples at 4 C for up to 48 hours and of serum samples at 4 C for up to 240 hours, despite appreciable pH changes, was associated with < 1.5% change in blood, plasma, and serum Ca2+ concentrations. Therefore, Ca2+ concentration in equine blood, plasma, and serum samples stored up to 48 hours and in serum samples stored up to 240 hours at 4 C is of diagnostic use.
Asunto(s)
Conservación de la Sangre/veterinaria , Calcio/sangre , Caballos/sangre , Animales , Femenino , Concentración de Iones de Hidrógeno , Plasma/química , Valores de Referencia , Temperatura , Factores de Tiempo , VenasRESUMEN
Stability of ionized calcium (Ca2+) concentration and pH values in bovine and ovine venous samples (n = 12 in each group) stored at 4 C for 3, 9, 24, and 48 hours (blood, plasma, and serum) or for 240 hours (plasma and serum), and at -20 C for 240 hours (plasma and serum), was studied. Storage of bovine and ovine blood, plasma, and serum samples at 4 C for up to 48 hours and of ovine serum samples at 4 C for up to 240 hours, despite appreciable pH changes, was associated with < 2.0% change in blood, plasma, and serum Ca2+ concentrations. Therefore, Ca2+ concentration in bovine and ovine venous blood, plasma, and serum samples stored up to 48 hours, and in ovine serum samples stored up to 240 hours, at 4 C is of diagnostic use.