Asunto(s)
Virus BK/fisiología , Papiloma/patología , Infecciones por Polyomavirus/patología , Infecciones Tumorales por Virus/patología , Neoplasias de la Vejiga Urinaria/patología , Adulto , Virus BK/genética , Secuencia de Bases , Trasplante de Médula Ósea , Clonación Molecular , Cistitis/microbiología , Cistitis/patología , ADN Viral , Humanos , Masculino , Microscopía Electrónica , Datos de Secuencia Molecular , Papiloma/microbiología , Infecciones por Polyomavirus/microbiología , Mapeo Restrictivo , Homología de Secuencia de Aminoácido , Infecciones Tumorales por Virus/microbiología , Neoplasias de la Vejiga Urinaria/microbiología , Neoplasias de la Vejiga Urinaria/ultraestructura , Replicación ViralRESUMEN
The efficacy of three different protein C activity assays and of protein C antigen determination for the diagnosis of protein C deficiency was studied in 13 protein C deficient patients (11 with type I, 2 with type II deficiency) and in 51 presumably non-deficient patients (control group), both groups being on oral anticoagulant (OAC) treatment. For protein C activity measurement (1) the assay according to Francis (slightly modified) with thrombin activation and measurement of activated protein C in the aPTT system, (2) an assay using Protac activation and chromogenic substrate (Protac-CS) and (3) an assay using Protac activation and the aPTT system (Protac-PTT) were used. Protein C antigen was determined by Laurell immunoelectrophoresis. The three activity assays gave different results, with the highest values obtained by the Protac-CS assay and the lowest values by the Protac-PTT assay. The Francis assay gave intermediate results. Protein C activity and antigen values were significantly lower in protein C deficient patients compared to the control group. Protein C activity tests had a higher discriminative power than the antigen determination. After taking into account the intensity of treatment, by the Francis assay all deficient and non-deficient patients were correctly classified, by the Protac-CS and the Protac-PTT assay 2 and 4 patients, respectively, were misclassified and by the antigen assay 8 patients were misclassified. Calculation of the ratios of protein C activity to factor II activity was of high discriminative power. We conclude that for diagnosis of protein C deficiency protein C activity tests are superior to antigen determination not only in type II but also in type I deficient patients.(ABSTRACT TRUNCATED AT 250 WORDS)
Asunto(s)
4-Hidroxicumarinas/uso terapéutico , Fenprocumón/uso terapéutico , Deficiencia de Proteína C , Trombosis/genética , Administración Oral , Humanos , Inmunoelectroforesis , Valor Predictivo de las Pruebas , Proteína C/metabolismo , Trombosis/sangre , Trombosis/tratamiento farmacológicoAsunto(s)
Fibrinógeno/análisis , Fibrinopéptido A/análisis , Leucemia Mieloide Aguda/sangre , Leucemia Mielomonocítica Aguda/sangre , Trombina/biosíntesis , Trastornos Hemorrágicos/etiología , Humanos , Leucemia Mieloide Aguda/complicaciones , Leucemia Mielomonocítica Aguda/complicaciones , Leucemia Promielocítica Aguda/sangre , Recuento de Leucocitos , Células Madre NeoplásicasRESUMEN
Intercellular junctions which are similar in ultrastructure and protein composition to typical desmosomes have so far only been found in epithelial cells and in heart tissue, specifically in the intercalated disks of cardiac myocytes and at cell boundaries between Purkinje fiber cells. In epithelial cells the cytoplasmic side of desmosomes, the 'desmosomal plaque', represents a specific attachment structure for the anchorage of intermediate filaments (IF) of the cytokeratin type. Cardiac myocytes do not contain cytokeratin filaments. In primary cultures of rat cardiac myocytes, we have examined by immunofluorescence and electron microscopy, using single and double label techniques, whether other types of IF are attached to the desmosomal plaques of the heart. Antibodies to desmoplakin, the major protein of the desmosomal plaque, have been used to label specifically the desmosomal plaques. It is shown that the desmoplakin-containing structures are often associated with IF stained by antibodies to desmin, i.e., the characteristic type of IF present in these cells. Like cytokeratin filaments in epithelial cells, desmin filaments attach laterally to the desmosomal plaque. They also remain attached to these plaques after endocytotic internalization of desmosomal domains by treatment of the cells with EGTA. These desmin filaments do not appear to attach to junctions of the fascia adherens type and to nexuses (gap junctions). These observations show that anchorage at desmosomal plaques is not restricted to IF of the cytokeratin type and that IF composed of either cytokeratin or desmin, specifically attach, in a lateral fashion, to desmoplakin-containing regions of the plasma membrane. We conclude that special domains exist in these two IF proteins that are involved in binding to the desmosomal plaque.