RESUMEN
BACKGROUND: Effective control of the use of anabolic-androgenic steroids (AASs) in animal sports is essential in order to ensure both animal welfare and integrity. In order to better police their use in Australian and New Zealand greyhound racing, thorough metabolic studies have been carried out on a range of registered human and veterinary AASs available in the region. RESULTS: Canine metabolic data are presented for the AASs boldenone, danazol, ethylestrenol, mesterolone, methandriol, nandrolone and norethandrolone. The principal Phase I metabolic processes observed were the reduction of A-ring unsaturations and/or 3-ketones with either 3α,5ß- or 3ß,5α-stereochemistry, the oxidation of secondary 17ß-hydroxyl groups and 16α-hydroxylation. The Phase II ß-glucuronylation of sterol metabolites was extensive. CONCLUSION: The presented data have enabled the effective analysis of AASs and their metabolites in competition greyhound urine samples.
Asunto(s)
Anabolizantes/metabolismo , Andrógenos/metabolismo , Esteroides/metabolismo , Anabolizantes/orina , Andrógenos/orina , Animales , Perros , Doping en los Deportes , Femenino , Masculino , Modelos Animales , Esteroides/orinaRESUMEN
The canine phase I and phase II metabolism of the synthetic anabolic-androgenic steroid stanozolol was investigated following intramuscular injection into a male greyhound. The major phase I biotransformation was hydroxylation to give 6alpha-hydroxystanozolol which was excreted as a glucuronide conjugate and was identified by comparison with synthetically derived reference materials. An analytical procedure was developed for the detection of this stanozolol metabolite in canine urine using solid phase extraction, enzyme hydrolysis of glucuronide conjugates and analysis by positive ion electrospray ionisation ion trap LC-MS.