RESUMEN
A case of Haemophilus influenzae type b meningitis in a neonate from whom both a beta-lactamase-negative and a beta-lactamase-positive strain were isolated simultaneously is reported. Both bacteriology laboratory technicians and clinicians should be aware of this phenomenon when performing sensitivity tests on H influenzae isolates or treating patients with serious H influenzae infection.
Asunto(s)
Haemophilus influenzae/aislamiento & purificación , Enfermedades del Recién Nacido/líquido cefalorraquídeo , Meningitis por Haemophilus/líquido cefalorraquídeo , Cloranfenicol/uso terapéutico , Haemophilus influenzae/enzimología , Humanos , Recién Nacido , Enfermedades del Recién Nacido/tratamiento farmacológico , Masculino , Meningitis por Haemophilus/tratamiento farmacológico , beta-Lactamasas/metabolismoAsunto(s)
Enfermedades de la Piel , Adolescente , Corynebacterium/aislamiento & purificación , Diagnóstico Diferencial , Hongos/aislamiento & purificación , Humanos , Micrococcaceae/aislamiento & purificación , Infecciones por Pseudomonas/diagnóstico , Infecciones por Pseudomonas/terapia , Piel/microbiología , Infecciones Estafilocócicas/diagnóstico , Infecciones Estafilocócicas/terapia , Infecciones Estreptocócicas/diagnóstico , Infecciones Estreptocócicas/terapia , SupuraciónRESUMEN
Newborn infants with "early-onset" disease due to group B beta hemolytic streptococcus were studied over a 40-month period. Clinical presentations included asymptomatic bacteremia, mild transient illness, respiratory distress, meningitis, and overwhelming sepsis. Chronologically, 18 were ill at birth; 10 became ill after a symptom-free period; and four were asymptomatic. Sixty-six percent of the cases weighted less than 2500 grams, and 56% were born to mothers whose amniotic membranes were ruptured for over 20 hours. All 15 of the deaths occurred in low birth weight infants who were criticially ill from birth. A review of 128 consecutive deliveries of infants weighing under 2000 grams revealed 28 cases with prolonged ruptured membranes, and three of these 28 infants developed group B streptococcal infection. The infant of the colonized gravid woman in premature labor or with prolonged ruptured membranes is clearly at risk, and these results suggest that the management of "early-onset" disease should begin prior to delivery.
Asunto(s)
Rotura Prematura de Membranas Fetales , Enfermedades del Recién Nacido , Infecciones Estreptocócicas , Femenino , Fiebre/complicaciones , Humanos , Recién Nacido de Bajo Peso , Recién Nacido , Embarazo , Infección Puerperal/complicaciones , Streptococcus agalactiae/aislamiento & purificaciónRESUMEN
The streptococcal long-chain reaction was adapted for the measurement of type-specific antibodies to group B beta-hemolytic streptococci (GBBHS). Rabbit antisera incubated with homologous but not heterologous GBBHS produced chains that were 18 to 33 times longer than chains produced by normal rabbit sera. The long chains were easily apparent, in most instances, by scanning the slides. Human sera with mouse protective and opsonic activity against GBBHS serotype Ia produced chains that were always significantly longer than those produced by incubation in nonimmune human sera. Absorption of rabbit or human sera with homologous but not heterologous organisms inhibited the capacity to induce the formation of long chains. The long-chain assay is a simple, rapid, and reproducible test that could constitute a valuable tool for the rapid identification of anti-GBBHS antibodies.
Asunto(s)
Anticuerpos Antibacterianos/análisis , Inmunoensayo , Streptococcus agalactiae/inmunología , Animales , Humanos , Proteínas Opsoninas , Conejos , Streptococcus agalactiae/crecimiento & desarrolloRESUMEN
Sera from 14 of 56 adult women protected mice from intraperitoneal challenge with mouse-virulent group B Streptococcus serotype Ia, and sera from seven of 25 nonparturient women in this group were bactericidal for greater than 99% of the organisms in the presence of normal polymorphonuclear leukocytes. There were no discrepancies between the in vivo and in vitro assays. Protective activity was found in the IgG class in seven sera, and in the IgM class in one. Opsonic activity was partially dependent on heat-labile serum factors. Of 31 mother-cord serum pairs studied, seven maternal sera were protective, but four of the corresponding cord sera were not. Pooled human gamma-globulin injected by either the intraperitoneal or the intramuscular route protected mice from bacterial challenge.
Asunto(s)
Anticuerpos Antibacterianos , Actividad Bactericida de la Sangre , Sangre Fetal/inmunología , Inmunoglobulina G/inmunología , Streptococcus agalactiae/inmunología , Adulto , Animales , Femenino , Humanos , Ratones , Neutrófilos/inmunología , Proteínas Opsoninas , Embarazo , gammaglobulinas/inmunologíaRESUMEN
The phagocytosis-induced metabolic burst of human newborn monocytes, as evaluated by their capacity to reduce NBT, was comparable to that of adult monocytes. The NBT reduction assay constitutes a simple method of ascertaining the functional capacity of human monocytes.