RESUMEN
The study determined the expression of cancer antigen (CA) 125 and HER-2 in 45 borderline ovarian tumors (BOTs) and investigated the correlation of these biologic markers with histologic type, clinical stage, and outcome. The level of CA 125 protein was assessed using DAKO's M-11 clone antibody in immunohistochemistry (IHC) assays (Carpinteria, CA). The HER-2 protein expression was assessed in IHC assays using the HercepTest (DAKO), and the HER-2 gene copy number per cell was investigated through fluorescence in situ hybridization (FISH) assays using VYSIS' PathVysion DNA Probe (Downers Grove, IL). Expression of the CA 125 protein was detected in 49% of the samples (22 out of 45 tumors) and significantly associated with the serous histologic type. However, CA 125 expression did not associate with clinical stage or outcome. Protein overexpression or gene amplification of HER-2 was not found. However, abnormal FISH results were detected in 16% (seven out of 45 patients) of specimens comprising extranumerary copies of HER-2 and/or chromosome 17 per cell. Abnormal FISH results were found to be independent of CA 125 expression and histologic type whereas they positively associate with advanced clinical stage. Our data show that HER-2 is not altered in BOTs, and the presence of aneusomy for chromosome 17 and HER-2 may predict tumor progression.
Asunto(s)
Biomarcadores de Tumor/metabolismo , Antígeno Ca-125/metabolismo , Neoplasias Ováricas/metabolismo , Receptor ErbB-2/metabolismo , Adenocarcinoma Mucinoso/metabolismo , Estudios de Casos y Controles , Cistadenocarcinoma Seroso/metabolismo , Progresión de la Enfermedad , Femenino , Regulación Neoplásica de la Expresión Génica , Humanos , Inmunohistoquímica , Hibridación Fluorescente in Situ , Adhesión en Parafina , Valor Predictivo de las Pruebas , Estudios RetrospectivosAsunto(s)
Transportadoras de Casetes de Unión a ATP/genética , Población Negra/genética , Evolución Molecular , Complejo Mayor de Histocompatibilidad , Miembro 3 de la Subfamilia B de Transportadores de Casetes de Unión a ATP , Alelos , Colombia , Exones/genética , Conversión Génica , Genes , Variación Genética , Haplotipos/genética , Humanos , Desequilibrio de Ligamiento , Polimorfismo Genético , Polimorfismo Conformacional Retorcido-SimpleRESUMEN
We describe a patient who acquired tungiasis while traveling in an area where the condition is endemic. The pathogenesis, diagnosis, and treatment of this tropical disease are presented.