RESUMEN
Geographically different strains of Borrelia burgdorferi sensu lato (B. burgdorferi sensu stricto Ir 105, B. burgdorferi s.s. + B. afzelii V 123, B. garinii Ir 112 - isolates from eastern Slovakia, B. garinii K24 - isolate from western Slovakia and B. burgdorferi s.s. B 31 - American strain) were compared as antigens for serological study of Lyme borreliosis by IgG ELISA on a group of horses from eastern Slovakia. In a set of 101 horse serum samples, positivity with the use of Ir 105 strain was 53 (52.4%), with V 123 51 (51.49%), with Ir 112 48 (47.5%), with K 24 47 (46.5%) and with B 31 only 25 (24.7%). The seroprevalence between strains B 31 and Ir 105, B 31 and V 123, B 31 and Ir 112, B 31 and K 24 differed statistically significantly (test chi2, p<0.05); however, the differences between strains Ir 105, V 123, Ir 112 and K24 were insignificant. Consistency of positive and negative findings between American and Slovak strains ranged from 50.5-62.4%. Comparison of Slovak strains (Ir105, V 123, Ir 112 and K 24) consistency of positive and negative findings was higher from 79.2-95.04%. The highest consistency of findings was reached comparing strains Ir 112 and K 24, and the same high agreement of results was observed between the strains Ir 105 and V 123 and also Ir 112 and Ir 105. Higher consistency of findings of serologically examined horses with geographically close trains is in accordance with greater similarity of protein profiles of Slovak strains compared to the American strain.
Asunto(s)
Grupo Borrelia Burgdorferi/clasificación , Enfermedades de los Caballos/inmunología , Enfermedades de los Caballos/microbiología , Ixodes/microbiología , Enfermedad de Lyme/microbiología , Enfermedad de Lyme/veterinaria , Animales , Antígenos Bacterianos/análisis , Grupo Borrelia Burgdorferi/inmunología , Reacciones Cruzadas , ADN Bacteriano/análisis , Ensayo de Inmunoadsorción Enzimática , Variación Genética , Enfermedades de los Caballos/diagnóstico , Enfermedades de los Caballos/transmisión , Caballos , Inmunoglobulina G/sangre , Insectos Vectores , Enfermedad de Lyme/diagnóstico , Enfermedad de Lyme/inmunología , Enfermedad de Lyme/transmisión , Reproducibilidad de los Resultados , Pruebas Serológicas , Eslovaquia , Especificidad de la EspecieRESUMEN
Saliva-activated transmission of Borrelia afzelii Canica, Nato, du Merle, Mazie, Baranton et Postic, 1993 was demonstrated using salivary gland extract (SGE) from Ixodes ricinus (L., 1758) ticks and C3H mice. Injection of Borrelia spirochaetes together with SGE increased the level of bacteraemia and accelerated the appearance of bacteria in the urinary bladder, compared with the injection of spirochaetes alone. More I. ricinus nymphs became infected when feeding on mice inoculated with B. afzelii plus SGE. Analysis of cytokines produced by cells of draining lymph nodes from SGE-treated mice showed a suppression of proinflammatory cytokines IFN-gamma, IL-6 and GM-CSF following a transient upregulation in comparison with the control mice infected without SGE.