RESUMEN

Fragment antigen-binding domains (Fabs) from anti-Frizzled and anti-LRP6 monoclonal antibodies were conjugated using SpyTag-SpyCatcher chemistry via a one-pot reaction. The resulting synthetic heterodimeric agonist outperformed the natural ligand, Wnt-3a, in activating canonical Wnt signaling in mammalian cells. This approach should be broadly applicable to activate receptor-mediated cellular signaling.

Asunto(s)

Anticuerpos Monoclonales/farmacología , Proteína Wnt3A/agonistas , Anticuerpos Monoclonales/química , Dimerización , Humanos , Ligandos , Estructura Molecular , Vía de Señalización Wnt/efectos de los fármacos