RESUMEN
Two fast migrating, major, multiple molecular forms (MMF) of glucose-6-phosphate dehydrogenase [EC:1.1.1.49]: G-6-PDH-1 and G-6-PDH-2, and two minor forms: G-6-PDH-3 and G-6-PDH-4 were revealed in the electrophoregrams of both erythrocytes haemolisates as well in the homogenates of bone marrow cellular lines of rats at control conditions. Daily 1 cGy irradiation of rats up to a cumulative dose of 20 cGy led to a drop of G-6-PDH total activity and it caused a redistribution of the MMF of the enzyme in bone marrow cellular populations. However, G-6-PDH activity in erythrocytes exceeded the control means in all the experimental terms. The calculation of the local redistribution coefficient (l(G-6-FDH-i)) showed that these changes are mainly determined by the increase of the activity of the isoform G-6-PDH-3. Vitamin D3 administration to rats generated a correction of G-6-PDH activity in all studied cellular populations. Meanwhile, the MMF profiles were characterized by multidirectional rearrangements in the bone marrow erythroid and granulocyte-monocyte cells and in erythrocytes. The specificity of changes in the distribution of the MMF of G-6-PDH in the three studied cellular populations depends on the particularities of their energetic metabolism at irradiation conditions and on the modifying action of the natural adaptogen 1,25-dihydroxicholecalciferol.
Asunto(s)
Adaptación Fisiológica , Colecalciferol/farmacología , Eritrocitos , Células Precursoras Eritroides , Glucosafosfato Deshidrogenasa/metabolismo , Irradiación Corporal Total , Adaptación Fisiológica/efectos de los fármacos , Adaptación Fisiológica/efectos de la radiación , Animales , Colecalciferol/administración & dosificación , Suplementos Dietéticos , Eritrocitos/efectos de los fármacos , Eritrocitos/enzimología , Eritrocitos/efectos de la radiación , Células Precursoras Eritroides/efectos de los fármacos , Células Precursoras Eritroides/enzimología , Células Precursoras Eritroides/efectos de la radiación , Dosis de Radiación , RatasRESUMEN
The long-term influence of low X-ray irradiation increases lipid peroxidation (LP) in radiosensitive (bone marrow, enterocytes of small intenstine) and in relatively radioresistant blood cells (erythrocytes). The activation of antioxidant system enzymes in observed cells does not decrease LP intensity. We concluded that additional administration of alpha-tocopherol provided the decrease of the first and end products of LP in the observed tissues mostly in the beginning of the experiment. Antioxidant effect of the preparation is more significant in cells with high proliferative activity but normal activity of enzymes was not determined.
Asunto(s)
Antioxidantes/uso terapéutico , Enterocitos/enzimología , Eritrocitos/enzimología , Intestino Delgado/enzimología , Traumatismos Experimentales por Radiación/prevención & control , Vitamina E/uso terapéutico , Animales , Células de la Médula Ósea/efectos de los fármacos , Células de la Médula Ósea/enzimología , Células de la Médula Ósea/efectos de la radiación , Catalasa/metabolismo , Relación Dosis-Respuesta en la Radiación , Enterocitos/efectos de los fármacos , Enterocitos/efectos de la radiación , Eritrocitos/efectos de los fármacos , Eritrocitos/efectos de la radiación , Glutatión Peroxidasa/metabolismo , Glutatión Reductasa/metabolismo , Intestino Delgado/efectos de los fármacos , Intestino Delgado/efectos de la radiación , Peróxidos Lipídicos/sangre , Dosis de Radiación , Traumatismos Experimentales por Radiación/sangre , Traumatismos Experimentales por Radiación/enzimología , Ratas , Superóxido Dismutasa/metabolismo , Rayos XRESUMEN
The effects of vitamin D3 (as an ingredient of the preparation "Videchol") on the activity of some glycolysis enzymes (lactic dehydrogenase, LDH; pyruvate kinase PK; hexokinase HK) and of pentose phosphate shunt (glucose-6-phosphate dehydrogenase), and also on the changes of metabolites concentration levels: ATP, NAD+, pyruvate and lactate following the administration of the vitamin to the control group rats and animals irradiated by low rate ionizing radiation were established. "Videchol" administration leads to the of LDH activity decrease within 10 days, of HK activity within 20 days and to the decrease of PK activity within 5 and 20 days after the experiment beginning. In the low dose radiation conditions a reconnection of carbohydrates' metabolism from glycolisys into pentose phosphate pathway in the erythrocytes was observed. The administration of vitamin D3 as a component of the preparation "Videchol" following the low rate radiation background facilitates the normalization of LDG, PK and HK activities in comparison with the same parameters of only irradiated rats. The studies on LDH--reaction kinetics showed the redistribution of the LDH isozymes pattern with different K(m) means for pyruvate in the rats subjected to the joint action of low dose chronical radiation and "Videchol" occurred.
Asunto(s)
Colecalciferol/farmacología , Eritrocitos/efectos de los fármacos , Animales , Eritrocitos/enzimología , Eritrocitos/metabolismo , Eritrocitos/efectos de la radiación , Femenino , Glucólisis/efectos de los fármacos , Glucólisis/efectos de la radiación , Hexoquinasa/sangre , L-Lactato Deshidrogenasa/sangre , Vía de Pentosa Fosfato , Piruvato Quinasa/sangre , Radiación Ionizante , RatasRESUMEN
The activity of glycolytic enzymes (lactate dehydrogenase, pyruvate kinase, hexokinase), content of metabolites (lactate, pyruvate, ATP, 2,3-DPG) and haemolytic stability of rat erythrocytes at the action of chronic X-irradiation at a daily dose 0.258 mC/kg, 2.58 mC/kg, 5.16 mC/kg during 90, 60 and 30 days, accordingly, have been investigated. It was shown, that the glycolytic enzymes activity of female rats in different seasons may vary in wide limits. A general tendency to the decrease of lactate dehydrogenase activity is revealed, but the beginning effects under the irradiation at a daily dose of 2.58 mC/kg and 5.16 mC/kg may be differently directed. The analogous tendencies are found in the change of hexokinase activity. The changes in pyruvate kinase activity correlated with erythrocytes haemolytic stability. The data obtained prove that changes in the direction of glycolytic enzymes activity under the irradiation depend on the initial level, which is caused by seasonal peculiarities of physiological state.