RESUMEN

This paper studies the effect of plant peptides of thionine Ns-W2 extracted from seeds of fennel flower (Nigella sativa) and ß-purothionine from wheat germs (Triticum kiharae), as well as a synthetic antimutagen (crown-compound), on the expression of several genes involved in the.control of cellular homeostasis, processes of carcinogenesis, and radiation response in human rhabdomyosarcoma cells (RD cells), T-lymphoblastoid cell line Jurkat, and blood cells. All of these agents acted as antimutagens-anticarcinogens, reducing the expression of genes involved in carcinogenesis (genes of families MMP, TIMP, and IAP and G-protein genes) in a tumor cell. A pronounced reduction in the mRNA level of these genes was caused by thionine Ns-W2, and the least effect was demonstrated by ß-purothionine. Antimutagens had very little effect on the mRNA levels of the several studied genes in normal blood cells.

Asunto(s)

Antimutagênicos/administración & dosificación , Péptidos/administración & dosificación , Fenotiazinas/administración & dosificación , Extractos Vegetales/administración & dosificación , Rabdomiosarcoma/genética , Antimutagênicos/química , Carcinogénesis/efectos de los fármacos , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Regulación Neoplásica de la Expresión Génica/efectos de los fármacos , Humanos , Nigella sativa/química , Péptidos/química , Fenotiazinas/química , Extractos Vegetales/química , Radiación Ionizante , Rabdomiosarcoma/tratamiento farmacológico , Rabdomiosarcoma/patología , Triticum/química , Proteína p53 Supresora de Tumor/biosíntesis