Asunto(s)
Conservación de los Recursos Naturales , Ecosistema , Desarrollo de la Planta , Animales , EcuadorRESUMEN
The prognostic significance of the epidermal growth factor receptor status (EGF-R-status) for certain human tumors requires the development of antibodies useful for clinical application. We used purified receptor preparations to generate monoclonal antibodies immunoreactive with the EGF-R purified from placenta membranes and A431 tumors. Four of the hybridomas contained antibodies (R2, R3, R5, and R9) which recognized both antigens. Antibody R3 was shown to display the following properties: it binds with a KD value of about 10(-9)-10(-10) M to the receptor, a half maximal inhibition of EGF-binding is achieved at 5 x 10(-8) M, and in Western blots of cell membranes R3 specifically detects the EGF-R at 0.1 micrograms/ml. R3 inhibits EGF-dependent clonogenic growth of NRK cells and completely blocks EGF stimulated autophosphorylation of the receptor. Moreover, R3 also detects EGF-R in paraffin-embedded tissue sections taken from human salivary gland, term placenta, and adult skin and mammary carcinomas. Thus, R3 can be used in retrospective diagnostic clinical studies and might help to develop new immunotherapeutic intervention.
Asunto(s)
Anticuerpos Monoclonales/inmunología , Receptores ErbB/análisis , Animales , Western Blotting/métodos , Neoplasias de la Mama/inmunología , Ensayo de Inmunoadsorción Enzimática , Receptores ErbB/inmunología , Receptores ErbB/metabolismo , Estudios de Evaluación como Asunto , Femenino , Humanos , Inmunohistoquímica/métodos , Ratones , Ratones Desnudos , Microtomía , Neoplasias Experimentales , Adhesión en Parafina , Fosforilación , Placenta/inmunología , Radioinmunoensayo , Estudios Retrospectivos , Neoplasias de las Glándulas Salivales/inmunología , Neoplasias Cutáneas/inmunología , Células Tumorales Cultivadas/efectos de los fármacosRESUMEN
Antimicrobial susceptibility patterns of Haemophilus isolates from children in eleven developing nations
Asunto(s)
Ampicilina/farmacología , Países en Desarrollo , Haemophilus/aislamiento & purificación , Haemophilus , Penicilina G/farmacología , Pruebas de Sensibilidad Microbiana/normasRESUMEN
Antimicrobial susceptibility patterns of Haemophilus isolates from children in eleven developing nations
Se publica en ingles en: Bull WHO 68(2), 1990