RESUMEN
PURPOSE: To investigate if low level laser therapy (LLLT) can decrease spinal cord injuries after temporary induced spinal cord ischemia-reperfusion in rats because of its anti-inflammatory effects. METHODS: Forty eight rats were randomized into two study groups of 24 rats each. In group I, ischemic-reperfusion (I-R) injury was induced without any treatment. Group II, was irradiated four times about 20 minutes for the following three days. The lesion site directly was irradiated transcutaneously to the spinal direction with 810 nm diode laser with output power of 150 mW. Functional recovery, immunohistochemical and histopathological changes were assessed. RESULTS: The average functional recovery scores of group II were significantly higher than that the score of group I (2.86 ± 0.68, vs 1.38 ± 0.09; p<0.05). Histopathologic evaluations in group II were showed a mild changes in compare with group I, that suggested this group survived from I-R consequences. Moreover, as seen from TUNEL results, LLLT also protected neurons from I-R-induced apoptosis in rats. CONCLUSION: Low level laser therapy was be able to minimize the damage to the rat spinal cord of reperfusion-induced injury.
Asunto(s)
Terapia por Luz de Baja Intensidad/métodos , Daño por Reperfusión/radioterapia , Traumatismos de la Médula Espinal/radioterapia , Isquemia de la Médula Espinal/radioterapia , Médula Espinal/irrigación sanguínea , Animales , Inmunohistoquímica , Etiquetado Corte-Fin in Situ , Láseres de Semiconductores/uso terapéutico , Masculino , Distribución Aleatoria , Ratas Wistar , Reproducibilidad de los Resultados , Traumatismos de la Médula Espinal/rehabilitación , Isquemia de la Médula Espinal/rehabilitación , Factores de Tiempo , Resultado del TratamientoRESUMEN
PURPOSE:To investigate if low level laser therapy (LLLT) can decrease spinal cord injuries after temporary induced spinal cord ischemia-reperfusion in rats because of its anti-inflammatory effects.METHODS: Forty eight rats were randomized into two study groups of 24 rats each. In group I, ischemic-reperfusion (I-R) injury was induced without any treatment. Group II, was irradiated four times about 20 minutes for the following three days. The lesion site directly was irradiated transcutaneously to the spinal direction with 810 nm diode laser with output power of 150 mW. Functional recovery, immunohistochemical and histopathological changes were assessed.RESULTS:The average functional recovery scores of group II were significantly higher than that the score of group I (2.86 ± 0.68, vs 1.38 ± 0.09; p<0.05). Histopathologic evaluations in group II were showed a mild changes in compare with group I, that suggested this group survived from I-R consequences. Moreover, as seen from TUNEL results, LLLT also protected neurons from I-R-induced apoptosis in rats.CONCLUSION:Low level laser therapy was be able to minimize the damage to the rat spinal cord of reperfusion-induced injury.
Asunto(s)
Animales , Masculino , Terapia por Luz de Baja Intensidad/métodos , Daño por Reperfusión/radioterapia , Traumatismos de la Médula Espinal/radioterapia , Isquemia de la Médula Espinal/radioterapia , Médula Espinal/irrigación sanguínea , Inmunohistoquímica , Etiquetado Corte-Fin in Situ , Láseres de Semiconductores/uso terapéutico , Distribución Aleatoria , Ratas Wistar , Reproducibilidad de los Resultados , Traumatismos de la Médula Espinal/rehabilitación , Isquemia de la Médula Espinal/rehabilitación , Factores de Tiempo , Resultado del TratamientoRESUMEN
BACKGROUND/AIMS: This study evaluated the effects of N-acetylcysteine as a scavenger of radical oxygen species on liver injury as a remote organ after skeletal muscle ischemia reperfusion. MATERIALS AND METHODS: Twenty male Wistar rats were allocated randomly into two experimental groups: ischemia reperfusion (I/R) and ischemia reperfusion + N-acetylcysteine (I/R+NAC). All animals were undergone 2h of ischemia by occlusion femoral artery and 24h of reperfusion. Rats that were treated with N-acetylcysteine given intravenously at a dose of 150 mg/kg, immediately before reperfusion. Serum levels of aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were measured. Livers were harvested for histopathological and biochemical studies. Liver tissue malondialdehyde (MDA), glutathione (GSH) and myeloperoxidase (MPO) activity were assayed. RESULTS: The ALT and AST values were significantly lower in I/R+NAC group. Hepatic MDA level and MPO activity were significantly increased in I/R group. The levels of GSH in liver tissue were significantly depressed by ischemia reperfusion. Liver histopathologic study in I/R group showed enlarged sinusoids, sinusoidal congestion, cytoplasmic vacuolation, cellular degenerative changes and necrosis. Histopathologically, there was a significant difference between two groups. CONCLUSION: Histopatological and biochemical results have shown that N-acetylcysteine was able to protect liver from skeletal muscle ischemia reperfusion injury.
Asunto(s)
Acetilcisteína/uso terapéutico , Depuradores de Radicales Libres/uso terapéutico , Hepatopatías/prevención & control , Músculo Esquelético/patología , Daño por Reperfusión/complicaciones , Alanina Transaminasa/sangre , Animales , Aspartato Aminotransferasas/sangre , Glutatión/metabolismo , Hepatopatías/metabolismo , Hepatopatías/patología , Masculino , Malondialdehído/metabolismo , Músculo Esquelético/irrigación sanguínea , Peroxidasa/metabolismo , Ratas , Ratas WistarRESUMEN
t is known that ischemia reperfusion causes remote organ injury as well as local injury. The aim of this study was to investigate whether N-acetylcysteine has a protective effect against testicular injury after skeletal muscle ischemia reperfusion. Twenty male Wistar rats were allocated to two groups: ischemia reperfusion (control group) and ischemia reperfusion + N-acetylcysteine (treatment group). All animals underwent 2 h of ischemia by occlusion of the femoral artery and 24 h of reperfusion. Rats in treatment group received N-acetylcysteine (150 mg/kg IV) before the reperfusion period. After the reperfusion period, testes were removed for histopathological and biochemical studies. The blood samples were collected for evaluation of serum malondialdehyde (MDA) and nitric oxide (NO) production levels. The MDA levels in testes homogenates were found to be significantly decreased in treatment group (p < 0.05). Treatment of N-acetylcysteine significantly decreased serum MDA and NO levels compared to the control group (p < 0.05). In the control group, tissues showed histological changes. Histopathologically, there was a significant difference (p < 0.05) between two groups. According to histological and biochemical findings, we conclude that N-acetylcysteine has preventive effects in the testicular injury after skeletal muscle ischemia reperfusion.
Asunto(s)
Acetilcisteína/farmacología , Antioxidantes/farmacología , Daño por Reperfusión/prevención & control , Testículo/patología , Animales , Masculino , Ratas , Ratas WistarRESUMEN
OBJECTIVE: To determine whether tramadol has a protective effect against lung injury induced by skeletal muscle ischemia-reperfusion. METHODS: Twenty Wistar male rats were allocated to one of two groups: ischemia-reperfusion (IR) and ischemia-reperfusion + tramadol (IR+T). The animals were anesthetized with intramuscular injections of ketamine and xylazine (50 mg/kg and 10 mg/kg, respectively). All of the animals underwent 2-h ischemia by occlusion of the femoral artery and 24-h reperfusion. Prior to the occlusion of the femoral artery, 250 IU heparin were administered via the jugular vein in order to prevent clotting. The rats in the IR+T group were treated with tramadol (20 mg/kg i.v.) immediately before reperfusion. After the reperfusion period, the animals were euthanized with pentobarbital (300 mg/kg i.p.), the lungs were carefully removed, and specimens were properly prepared for histopathological and biochemical studies. RESULTS: Myeloperoxidase activity and nitric oxide levels were significantly higher in the IR group than in the IR+T group (p = 0.001 for both). Histological abnormalities, such as intra-alveolar edema, intra-alveolar hemorrhage, and neutrophil infiltration, were significantly more common in the IR group than in the IR+T group. CONCLUSIONS: On the basis of our histological and biochemical findings, we conclude that tramadol prevents lung tissue injury after skeletal muscle ischemia-reperfusion.
Asunto(s)
Lesión Pulmonar/prevención & control , Pulmón/patología , Músculo Esquelético/irrigación sanguínea , Daño por Reperfusión/prevención & control , Tramadol/uso terapéutico , Animales , Modelos Animales de Enfermedad , Arteria Femoral , Lesión Pulmonar/etiología , Lesión Pulmonar/patología , Masculino , Óxido Nítrico/análisis , Peroxidasa/análisis , Distribución Aleatoria , Ratas , Ratas Wistar , Daño por Reperfusión/complicaciones , Daño por Reperfusión/patologíaRESUMEN
PURPOSE: To evaluate the bone repair process in ovariohysterectomized rabbit submitted to an ovarian transplant to stomach that may supplying some quantity of estrogen occurs to improve bone healing. METHODS: In 20 female rabbits three holes of 1, 2 and 3mm diameter in tibial shaft were made and after that all animals received OHE through a ventral incision and they were randomly divided into two groups of ten rabbits each. In group one, animals received one of their self-ovaries that transplanted on serosal layer of stomach and group two did not receive treatment. Animals were kept during bone healing for a period of 45 days and radiological, biochemical, biomechanical and histopathological evaluation. RESULTS: The tibial defects in group one healed completely after 45 days and had more callous than second group. There is significant difference between two groups after operation in 21, 28 and 35 days about estrogen, progesterone and phosphatase Alkaline. The maximum forces in group one, were significantly higher than that for the group two. CONCLUSION: Ovarian transplantation prevents the effects of ovariohysterectomized on bone healing of rabbit tibia, suggesting that unilateral transplanted ovaries can substitute for the action of ovaries on the skeleton in ovariohysterectomized animals.
Asunto(s)
Regeneración Ósea/fisiología , Histerectomía , Ovariectomía , Ovario/trasplante , Estómago/cirugía , Tibia/lesiones , Cicatrización de Heridas/fisiología , Fosfatasa Alcalina/metabolismo , Animales , Estrógenos/metabolismo , Femenino , Progesterona/metabolismo , Conejos , Factores de TiempoRESUMEN
PURPOSE: To investigate if the methanolic extract of the Otostegia persica can accelerating healing process of burn wound because of its anti-inflammatory and antioxidant effects. METHODS: Forty eight male Wistar rats were randomized into three study groups of 16 rats each. Burn wounds were created on dorsal part of shaved rats using a metal rod. In group I the burn wound was left without any treatment. Group was treated with topical silver sulfadiazine pomade. In group III, ointment containing the OP extract was administered. Skin biopsies were harvested from burn area on the 3rd, 5th, 14th and 21st days after burn and examined histologically. RESULTS: Re-epithelialization in the control group and in group II was lower than in group III. Re-epithelialization in groups II and III was significantly different from that in the control group. On the 5th day of the experiment, we assessed lower inflammation in the burn area compared to control group. This means that the inflammation was suppressed by methanolic extract of OP. From day 5 to 14; the fibroblast proliferation peaked and was associated with increased collagen accumulation. It was obvious that angiogenesis improved more in the groups II and III, which facilitated re-epithelialisation. CONCLUSION: Methanolic extract of Otostegia persica exhibited significant healing activity when topically applied on rats. OP is an effective treatment for saving the burn site.
Asunto(s)
Antiinflamatorios/uso terapéutico , Antioxidantes/uso terapéutico , Quemaduras/tratamiento farmacológico , Lamiaceae/química , Extractos Vegetales/uso terapéutico , Cicatrización de Heridas/efectos de los fármacos , Animales , Biopsia , Quemaduras/patología , Masculino , Distribución Aleatoria , Ratas , Ratas Wistar , Reproducibilidad de los Resultados , Piel/efectos de los fármacos , Piel/patología , Factores de Tiempo , Resultado del TratamientoRESUMEN
PURPOSE: To investigate if the methanolic extract of the Otostegia persica can accelerating healing process of burn wound because of its anti-inflammatory and antioxidant effects. METHODS:Forty eight male Wistar rats were randomized into three study groups of 16 rats each. Burn wounds were created on dorsal part of shaved rats using a metal rod. In group I the burn wound was left without any treatment. Group was treated with topical silver sulfadiazine pomade. In group III, ointment containing the OP extract was administered. Skin biopsies were harvested from burn area on the 3rd, 5th, 14th and 21st days after burn and examined histologically. RESULTS: Re-epithelialization in the control group and in group II was lower than in group III. Re-epithelialization in groups II and III was significantly different from that in the control group. On the 5th day of the experiment, we assessed lower inflammation in the burn area compared to control group. This means that the inflammation was suppressed by methanolic extract of OP. From day 5 to 14; the fibroblast proliferation peaked and was associated with increased collagen accumulation. It was obvious that angiogenesis improved more in the groups II and III, which facilitated re-epithelialisation. CONCLUSION:Methanolic extract of Otostegia persica exhibited significant healing activity when topically applied on rats. OP is an effective treatment for saving the burn site.
Asunto(s)
Animales , Masculino , Ratas , Antiinflamatorios/uso terapéutico , Antioxidantes/uso terapéutico , Quemaduras/tratamiento farmacológico , Lamiaceae/química , Extractos Vegetales/uso terapéutico , Cicatrización de Heridas/efectos de los fármacos , Biopsia , Quemaduras/patología , Distribución Aleatoria , Ratas Wistar , Reproducibilidad de los Resultados , Piel/efectos de los fármacos , Piel/patología , Factores de Tiempo , Resultado del TratamientoRESUMEN
PURPOSE: To evaluate the bone repair process in ovariohysterectomized rabbit submitted to an ovarian transplant to stomach that may supplying some quantity of estrogen occurs to improve bone healing. METHODS: In 20 female rabbits three holes of 1, 2 and 3mm diameter in tibial shaft were made and after that all animals received OHE through a ventral incision and they were randomly divided into two groups of ten rabbits each. In group one, animals received one of their self-ovaries that transplanted on serosal layer of stomach and group two did not receive treatment. Animals were kept during bone healing for a period of 45 days and radiological, biochemical, biomechanical and histopathological evaluation. RESULTS: The tibial defects in group one healed completely after 45 days and had more callous than second group. There is significant difference between two groups after operation in 21, 28 and 35 days about estrogen, progesterone and phosphatase Alkaline. The maximum forces in group one, were significantly higher than that for the group two. CONCLUSION:Ovarian transplantation prevents the effects of ovariohysterectomized on bone healing of rabbit tibia, suggesting that unilateral transplanted ovaries can substitute for the action of ovaries on the skeleton in ovariohysterectomized animals.
Asunto(s)
Animales , Femenino , Conejos , Regeneración Ósea/fisiología , Histerectomía , Ovariectomía , Ovario/trasplante , Estómago/cirugía , Tibia/lesiones , Cicatrización de Heridas/fisiología , Fosfatasa Alcalina/metabolismo , Estrógenos/metabolismo , Progesterona/metabolismo , Factores de TiempoRESUMEN
OBJECTIVE: To determine whether tramadol has a protective effect against lung injury induced by skeletal muscle ischemia-reperfusion. METHODS: Twenty Wistar male rats were allocated to one of two groups: ischemia-reperfusion (IR) and ischemia-reperfusion + tramadol (IR+T). The animals were anesthetized with intramuscular injections of ketamine and xylazine (50 mg/kg and 10 mg/kg, respectively). All of the animals underwent 2-h ischemia by occlusion of the femoral artery and 24-h reperfusion. Prior to the occlusion of the femoral artery, 250 IU heparin were administered via the jugular vein in order to prevent clotting. The rats in the IR+T group were treated with tramadol (20 mg/kg i.v.) immediately before reperfusion. After the reperfusion period, the animals were euthanized with pentobarbital (300 mg/kg i.p.), the lungs were carefully removed, and specimens were properly prepared for histopathological and biochemical studies. RESULTS: Myeloperoxidase activity and nitric oxide levels were significantly higher in the IR group than in the IR+T group (p = 0.001 for both). Histological abnormalities, such as intra-alveolar edema, intra-alveolar hemorrhage, and neutrophil infiltration, were significantly more common in the IR group than in the IR+T group. CONCLUSIONS: On the basis of our histological and biochemical findings, we conclude that tramadol prevents lung tissue injury after skeletal muscle ischemia-reperfusion. .
OBJETIVO: Investigar se o tramadol tem um efeito protetor contra a lesão pulmonar induzida por isquemia-reperfusão de músculo esquelético. MÉTODOS: Vinte ratos Wistar machos foram divididos em dois grupos: grupo isquemia-reperfusão (IR) e grupo isquemia-reperfusão + tramadol (IR+T). Os animais foram anestesiados com cetamina e xilazina (i.m., 50 mg/kg e 10 mg/kg, respectivamente). Todos os animais foram submetidos a 2 h de isquemia por oclusão da artéria femoral e 24 h de reperfusão. Antes da oclusão da artéria femoral, foram administrados 250 UI de heparina pela veia jugular para impedir a coagulação. Os ratos do grupo IR+T foram tratados com tramadol (20 mg/kg i.v.) imediatamente antes da reperfusão. Após o período de reperfusão, os animais foram sacrificados com pentobarbital (300 mg/kg i.p.), os pulmões foram removidos cuidadosamente, e os espécimes foram preparados adequadamente para estudos histopatológicos e bioquímicos. RESULTADOS: A atividade de mieloperoxidase e os níveis de óxido nítrico foram significativamente maiores no grupo IR que no grupo IR+T (p = 0,001 para ambos). Anormalidades histológicas, como edema intra-alveolar, hemorragia intra-alveolar e infiltração neutrofílica, foram significativamente mais frequentes no grupo IR que no grupo IR+T. CONCLUSÕES: Com base nos resultados histológicos e bioquímicos ...
Asunto(s)
Animales , Masculino , Ratas , Lesión Pulmonar/prevención & control , Pulmón/patología , Músculo Esquelético/irrigación sanguínea , Daño por Reperfusión/prevención & control , Tramadol/uso terapéutico , Modelos Animales de Enfermedad , Arteria Femoral , Lesión Pulmonar/etiología , Lesión Pulmonar/patología , Óxido Nítrico/análisis , Peroxidasa/análisis , Distribución Aleatoria , Ratas Wistar , Daño por Reperfusión/complicaciones , Daño por Reperfusión/patologíaRESUMEN
PURPOSE: Articular Cartilage has limited potential for self-repair and tissue engineering approaches attempt to repair articular cartilage by scaffolds. We hypothesized that the combined hydroxyapatite and zirconia stabilized yttria would enhance the quality of cartilage healing. METHODS: In ten New Zealand white rabbits bilateral full-thickness osteochondral defect, 4 mm in diameter and 3 mm depth, was created on the articular cartilage of the patellar groove of the distal femur. In group I the scaffold was implanted into the right stifle and the same defect was created in the left stifle without any transplant (group II). Specimens were harvested at 12 weeks after implantation, examined histologically for morphologic features, and stained immunohistochemically for type-II collagen. RESULTS: In group I the defect was filled with a white translucent cartilage tissue In contrast, the defects in the group II remained almost empty. In the group I, the defects were mostly filled with hyaline-like cartilage evidenced but defects in group II were filled with fibrous tissue with surface irregularities. Positive immunohistochemical staining of type-II collagen was observed in group I and it was absent in the control group. CONCLUSION: The hydroxyapatite/yttria stabilized zirconia scaffold would be an effective scaffold for cartilage tissue engineering.
Asunto(s)
Materiales Biocompatibles/uso terapéutico , Cartílago Articular/lesiones , Durapatita/uso terapéutico , Nanoestructuras/uso terapéutico , Cicatrización de Heridas/efectos de los fármacos , Itrio/uso terapéutico , Circonio/uso terapéutico , Animales , Colágeno Tipo II/análisis , Masculino , Ensayo de Materiales , Conejos , Regeneración/efectos de los fármacos , Reproducibilidad de los Resultados , Propiedades de Superficie , Factores de Tiempo , Resultado del TratamientoRESUMEN
PURPOSE: Articular Cartilage has limited potential for self-repair and tissue engineering approaches attempt to repair articular cartilage by scaffolds. We hypothesized that the combined hydroxyapatite and zirconia stabilized yttria would enhance the quality of cartilage healing. METHODS: In ten New Zealand white rabbits bilateral full-thickness osteochondral defect, 4 mm in diameter and 3 mm depth, was created on the articular cartilage of the patellar groove of the distal femur. In group I the scaffold was implanted into the right stifle and the same defect was created in the left stifle without any transplant (group II). Specimens were harvested at 12 weeks after implantation, examined histologically for morphologic features, and stained immunohistochemically for type-II collagen. RESULTS: In group I the defect was filled with a white translucent cartilage tissue In contrast, the defects in the group II remained almost empty. In the group I, the defects were mostly filled with hyaline-like cartilage evidenced but defects in group II were filled with fibrous tissue with surface irregularities. Positive immunohistochemical staining of type-II collagen was observed in group I and it was absent in the control group. CONCLUSION: The hydroxyapatite/yttria stabilized zirconia scaffold would be an effective scaffold for cartilage tissue engineering.
Asunto(s)
Animales , Masculino , Conejos , Materiales Biocompatibles/uso terapéutico , Cartílago Articular/lesiones , Durapatita/uso terapéutico , Nanoestructuras/uso terapéutico , Cicatrización de Heridas/efectos de los fármacos , Itrio/uso terapéutico , Circonio/uso terapéutico , Colágeno Tipo II/análisis , Ensayo de Materiales , Reproducibilidad de los Resultados , Regeneración/efectos de los fármacos , Propiedades de Superficie , Factores de Tiempo , Resultado del TratamientoRESUMEN
PURPOSE: To investigate the osteoconductive properties and biological performance of Poly L-lactic acid (PLLA) with omentum in bone defects. METHODS: PLLA nanofiber scaffolds were prepared via electrospinning technique. Forty four New Zealand white female rabbits randomly divided into three groups of 18 rabbits each. Created defects in right tibias were filled in group I with omentum, in group II with PLLA nanofiber scaffold and in group III with combination of the omentum and PLLA. The same defects were created in left tibia of all groups but did not receive any treatment (control group). Histological and histomorphometric evaluations were performed at two, four and six weeks after the implantation. RESULTS: Histological changes on all groups along with the time course were scored and statistical analysis showed that the average scores in group III were significantly higher than the other groups. CONCLUSION: Histomorphometric analysis of bone healing was shown to be significantly improved by the combined PLLA with omentum compared with the other groups, suggesting this biomaterial promote the healing of cortical bone, presumably by acting as an osteoconductive scaffold.
Asunto(s)
Regeneración Ósea/efectos de los fármacos , Ácido Láctico/uso terapéutico , Nanofibras/uso terapéutico , Epiplón/trasplante , Polímeros/uso terapéutico , Tibia/lesiones , Cicatrización de Heridas/efectos de los fármacos , Animales , Materiales Biocompatibles/uso terapéutico , Femenino , Ensayo de Materiales , Microscopía Electrónica de Rastreo , Modelos Animales , Poliésteres , Conejos , Factores de Tiempo , Andamios del Tejido , Resultado del TratamientoRESUMEN
PURPOSE: To investigate the osteoconductive properties and biological performance of Poly L-lactic acid (PLLA) with omentum in bone defects. METHODS: PLLA nanofiber scaffolds were prepared via electrospinning technique. Forty four New Zealand white female rabbits randomly divided into three groups of 18 rabbits each. Created defects in right tibias were filled in group I with omentum, in group II with PLLA nanofiber scaffold and in group III with combination of the omentum and PLLA. The same defects were created in left tibia of all groups but did not receive any treatment (control group). Histological and histomorphometric evaluations were performed at two, four and six weeks after the implantation. RESULTS: Histological changes on all groups along with the time course were scored and statistical analysis showed that the average scores in group III were significantly higher than the other groups. CONCLUSION: Histomorphometric analysis of bone healing was shown to be significantly improved by the combined PLLA with omentum compared with the other groups, suggesting this biomaterial promote the healing of cortical bone, presumably by acting as an osteoconductive scaffold.
OBJETIVO: Investigar as propriedades de osteocondução e desempenho biológico do ácido L láctico-Poly (PLLA) com omento em defeitos ósseos. MÉTODOS: Andaimes PLLA nanofibras foram preparados via eletrofiação técnica. Cinquenta e quatro coelhos fêmeas Nova Zelândia brancos foram distribuídos aleatoriamente em três grupos de 18 coelhos cada. Defeitos criados em tíbias direitas foram preenchidos no grupo I com omento, no grupo II com PLLA nanofibras e no grupo III com a combinação do omento e PLLA. Os mesmos defeitos foram criados na tíbia esquerda de todos os grupos, mas não receberam qualquer tratamento (grupo controle). As avaliações histológicas e histomorfométricas foram realizadas em duas, quatro e seis semanas após a implantação. RESULTADOS: As alterações histológicas em todos os grupos, juntamente com o curso de tempo foram marcados e análise estatística mostrou que as pontuações médias do grupo III foram significativamente mais elevadas do que os outros grupos. CONCLUSÃO: Análise histomorfométrica da cicatrização óssea mostrou-se significativamente melhor com o PLLA combinado com omento em comparação com os outros grupos, sugerindo que este biomaterial promove a cicatrização do osso cortical, provavelmente atuando como osteocondutor.
Asunto(s)
Animales , Femenino , Conejos , Regeneración Ósea/efectos de los fármacos , Ácido Láctico/uso terapéutico , Nanofibras/uso terapéutico , Epiplón/trasplante , Polímeros/uso terapéutico , Tibia/lesiones , Cicatrización de Heridas/efectos de los fármacos , Materiales Biocompatibles/uso terapéutico , Ensayo de Materiales , Microscopía Electrónica de Rastreo , Modelos Animales , Factores de Tiempo , Andamios del Tejido , Resultado del TratamientoRESUMEN
PURPOSE: To investigate whether N-acetylcysteine has a protective effect against renal injury as a remote organ after skeletal muscle ischemia-reperfusion in rats. METHODS: Twenty Wistar male rats were divided randomly into two experimental groups: group ischemia-reperfusion (group I) and group ischemia-reperfusion + N-acetylcysteine (group II). After ketamine and xylazine anesthesia, femoral artery was exposed. All animals were undergone 2h of ischemia by occlusion femoral artery and 24h of reperfusion. Rats that were treated with N-acetylcysteine given IV at a dose of 150 mg/kg(-1), immediately before reperfusion. After 24h of reperfusion, the blood samples were collected and submitted for evaluation of plasmatic urea, creatinine values and then rats were euthanized and left kidney harvested for histopathological analysis under light microscopy. RESULTS: The urea (35±7.84 mg.dL(-1)), creatinine (1.46±0.47 mg.dL(-1)) values were significantly lower in group II (P=0.000). Renal histopathologic study in group I showed extensive distal and proximal tubular cells necrosis and sloughing of epithelial cells into the tubular lumen, cast formation in tubule and glomerul, glomerul fibrosis and hemorrhage. Histopathologically, there was a significant difference (p=0.037) between two groups. CONCLUSION: The N-acetylcysteine was able to decrease renal injury induced by skeletal muscle ischemia reperfusion in rats.
Asunto(s)
Acetilcisteína/uso terapéutico , Lesión Renal Aguda/prevención & control , Isquemia/complicaciones , Músculo Esquelético/irrigación sanguínea , Daño por Reperfusión/prevención & control , Lesión Renal Aguda/etiología , Animales , Creatinina/sangre , Masculino , Distribución Aleatoria , Ratas , Ratas Wistar , Daño por Reperfusión/sangre , Daño por Reperfusión/etiologíaRESUMEN
PURPOSE: To investigate whether N-acetylcysteine has a protective effect against renal injury as a remote organ after skeletal muscle ischemia-reperfusion in rats. METHODS: Twenty Wistar male rats were divided randomly into two experimental groups: group ischemia-reperfusion (group I) and group ischemia-reperfusion + N-acetylcysteine (group II). After ketamine and xylazine anesthesia, femoral artery was exposed. All animals were undergone 2h of ischemia by occlusion femoral artery and 24h of reperfusion. Rats that were treated with N-acetylcysteine given IV at a dose of 150 mg/kg-¹, immediately before reperfusion. After 24h of reperfusion, the blood samples were collected and submitted for evaluation of plasmatic urea, creatinine values and then rats were euthanized and left kidney harvested for histopathological analysis under light microscopy. RESULTS: The urea (35±7.84 mg.dL-1), creatinine (1.46±0.47 mg.dL-1) values were significantly lower in group II (P=0.000). Renal histopathologic study in group I showed extensive distal and proximal tubular cells necrosis and sloughing of epithelial cells into the tubular lumen, cast formation in tubule and glomerul, glomerul fibrosis and hemorrhage. Histopathologically, there was a significant difference (p=0.037) between two groups. CONCLUSION: The N-acetylcysteine was able to decrease renal injury induced by skeletal muscle ischemia reperfusion in rats.
OBJETIVO: Investigar se a N-acetilcisteína tem um efeito protetor contra a lesão renal como um órgão remoto músculo esquelético após isquemia-reperfusão em ratos. MÉTODOS: Vinte ratos Wistar machos foram distribuídos aleatoriamente em dois grupos experimentais: grupo isquemia-reperfusão (grupo I) e grupo isquemia-reperfusão N-acetilcisteína (grupo II). Após a anestesia de ketamina e xilazina, a artéria femoral foi exposta. Todos os animais foram submetidos a 2h de isquemia pela oclusão da artéria femoral e 24h de reperfusão. Os ratos que foram tratados com N-acetilcisteína administrados IV na dose de 150 mgkg-1, imediatamente antes da reperfusão. Após 24h de reperfusão, as amostras de sangue foram coletadas e submetidas para avaliação de uréia, creatinina e, em seguida, os ratos foram sacrificados e rim esquerdo retirados para estudo histopatológico em microscopia de luz. RESULTADOS: A uréia (35 ± 7,84 mg.dL-1), creatinina (1,46 ± 0,47 mg.dL-1) os valores foram significativamente menores no grupo II (p=0,000). Estudo histopatológico renal do grupo I mostrou extensa necrose distal e proximal, células tubular e descamação das células epiteliais para o lúmen tubular, formação de elenco no túbulo e glomerulo, fibrose glomerular e hemorragia. Histopatologicamente houve uma diferença significativa (p=0,037) entre os dois grupos. CONCLUSÃO: A N-acetilcisteína foi capaz de diminuir a lesão renal induzida por reperfusão de isquemia do músculo esquelético em ratos.
Asunto(s)
Animales , Masculino , Ratas , Acetilcisteína/uso terapéutico , Lesión Renal Aguda/prevención & control , Isquemia/complicaciones , Músculo Esquelético/irrigación sanguínea , Daño por Reperfusión/prevención & control , Lesión Renal Aguda/etiología , Creatinina/sangre , Distribución Aleatoria , Ratas Wistar , Daño por Reperfusión/sangre , Daño por Reperfusión/etiologíaRESUMEN
PURPOSE: To investigate whether N-acetylcysteine, a free radicals scavenger has a protective effect against lung injury as a remote organ after skeletal muscle ischemia-reperfusion. METHODS: Twenty Wistar male rats were divided randomly into two experimental groups: group ischemia-reperfusion (group I) and group ischemia-reperfusion +N-acetylcysteine (group II). All animals were undergone two hours of ischemia by occlusion femoral artery and 24h of reperfusion. Before clamped the femoral artery, 250 IU heparin was administered via the jugular vein to prevent clotting. Rats that were treated with N-acetylcysteine given IV at a dose of 150 mgkg(-1), immediately before reperfusion. After 24h of reperfusion, animals were euthanized and left lung harvested for histopathological analysis under light microscopy. RESULTS: In the group I, tissues showed histological changes with intra-alveolar edema, intra-alveolar hemorrhage and neutrophilic infiltration. Histopathologically, there was a significant difference (P = 0.005) between two groups. CONCLUSION: Administration of N-acetylcysteine treatment significantly decreased lung injury induced by skeletal muscle ischemia reperfusion according to histological findings.
Asunto(s)
Acetilcisteína/uso terapéutico , Modelos Animales de Enfermedad , Depuradores de Radicales Libres/uso terapéutico , Lesión Pulmonar/prevención & control , Músculo Esquelético , Daño por Reperfusión/prevención & control , Animales , Pulmón/efectos de los fármacos , Pulmón/patología , Lesión Pulmonar/patología , Masculino , Distribución Aleatoria , Ratas , Ratas Wistar , Daño por Reperfusión/patología , Factores de Tiempo , Resultado del TratamientoRESUMEN
PURPOSE: To investigate whether N-acetylcysteine, a free radicals scavenger has a protective effect against lung injury as a remote organ after skeletal muscle ischemia-reperfusion. METHODS: Twenty Wistar male rats were divided randomly into two experimental groups: group ischemia-reperfusion (group I) and group ischemia-reperfusion + N-acetylcysteine (group II). All animals were undergone two hours of ischemia by occlusion femoral artery and 24h of reperfusion. Before clamped the femoral artery, 250 IU heparin was administered via the jugular vein to prevent clotting. Rats that were treated with N-acetylcysteine given IV at a dose of 150 mgkg-¹, immediately before reperfusion. After 24h of reperfusion, animals were euthanized and left lung harvested for histopathological analysis under light microscopy. RESULTS: In the group I, tissues showed histological changes with intra-alveolar edema, intra-alveolar hemorrhage and neutrophilic infiltration. Histopathologically, there was a significant difference (P = 0.005) between two groups. CONCLUSION: Administration of N-acetylcysteine treatment significantly decreased lung injury induced by skeletal muscle ischemia reperfusion according to histological findings.
OBJETIVO: Investigar se N-acetilcisteína, neutralizador de radicais livres, tem efeito protetor contra dano pulmonar como um órgão remoto após isquemia-reperfusão de músculo esquelético. MÉTODOS: Vinte ratos machos Wistar, foram aleatóriamente distribuídos em dois grupos: grupo isquemia-reperfusão (grupo I) e grupo isquemia-reperfusão +N-acetilcisteína (grupo II). Todos os animais foram submetidos a duas horas de ischemia pela oclusão artéria femoral e 24 horas de reperfusão. Antes de ocluir a artéria femoral, foi administrado 250 IU de heparina pela veia jugular para prevenir coagulação. A N-acetilcisteína foi administrada por via intravenosa, na uma dose de 150 mgkg-1, imediatamente antes de reperfusão. Após 24 horas de reperfusão, os animais foram eutanasiados e o pulmão esquerdo foi removido para análise histológica em microscopia óptica. RESULTADOS: No grupo I, os tecidos mostraram alterações histológicas com edema e hemorragia intra-alveolar e infiltração neutrofílica. Houve diferença histopatológica significante (P = 0.005) entre os dois grupos. CONCLUSÃO: O tratamento com a N-acetilcisteína diminuiu significantemente o dano pulmonar induzido por isquemia-reperfusão de músculo esquelético.
Asunto(s)
Animales , Masculino , Ratas , Acetilcisteína/uso terapéutico , Modelos Animales de Enfermedad , Depuradores de Radicales Libres/uso terapéutico , Lesión Pulmonar/prevención & control , Músculo Esquelético , Daño por Reperfusión/prevención & control , Lesión Pulmonar/patología , Pulmón/efectos de los fármacos , Pulmón/patología , Distribución Aleatoria , Ratas Wistar , Daño por Reperfusión/patología , Factores de Tiempo , Resultado del TratamientoRESUMEN
Articular cartilage has a limited capacity for self-repair. Untreated injuries of cartilage may lead to osteoarthritis. This problem demands new effective methods to reconstruct articular cartilage. Mesenchymal stem cells (MSCs) have the proclivity to differentiate along multiple lineages giving rise to new bone, cartilage, muscle, or fat. This study was an animal model for autologous effects of transplantation of MSCs with a collagen-poly(vinyl alcohol) (PVA) scaffold into full-thickness osteochondral defects of the stifle joint in the rabbit as an animal model. A group of 10 rabbits had a defect created experimentally in the full thickness of articular cartilage penetrated into the subchondral space in the both stifle joints. The defect in the right stifle was filled with MSCs/collagen-PVA scaffold (group I), and in the left stifle, the defect was left without any treatment as the control group (group II). Specimens were harvested at 12 weeks after implantation, examined histologically for morphologic features, and stained immunohistochemically for type-II collagen. Histology observation showed that the MSCs/collagen-PVA repair group had better chondrocyte morphology, continuous subchondral bone, and much thicker newly formed cartilage compared with the control group at 12 weeks post operation. There was a significant difference in histological grading score between these two groups. The present study suggested that the hybrid collagen-PVA scaffold might serve as a new way to keep the differentiation of MSCs for enhancing cartilage repair.