RESUMEN
Hydrothermal liquefaction is a promising method to convert municipal sludge into an energy-dense fuel. The inevitable by-product aqueous phase is rich in complex organics, which has the potential for energy and nutrient recovery and can be treated by anaerobic digestion to produce methane. However, toxic compounds such as ammonia and phenolics present would inhibit the function of micro-organisms. This study investigated the influence of ammonia and phenolics removal on anaerobic digestion. The results showed that the treated aqueous phase resulted in up to 225 ml CH4/g COD. The highest methane production was obtained in the culture with both ammonia and phenolics removal at pH 7.0, which was about 90% higher than only ammonia removal and seven times higher than only phenolics removal. The microbial community analysis results showed that these two treatments could increase microbial diversity and upregulate the relative abundance of methanogens.