RESUMEN
The therapeutic effect of a Polar shark cartilage preparation which is an enzymatic hydrolysate was studied in a rabbit model of infective allergic pseudotuberculous arthritis. Characterization of the chemical composition of the preparation designed by an original method is presented. Improvement of the general state of the affected joints and development of tissue immunomorphological responses were shown.
Asunto(s)
Artritis Experimental/tratamiento farmacológico , Artritis Reactiva/tratamiento farmacológico , Cartílago , Extractos de Tejidos/uso terapéutico , Animales , Artritis Experimental/patología , Artritis Reactiva/patología , Productos Biológicos/química , Productos Biológicos/aislamiento & purificación , Productos Biológicos/uso terapéutico , Cartílago/química , Hidrólisis , Conejos , Tiburones , Extractos de Tejidos/química , Yersinia pseudotuberculosisRESUMEN
Monocytes/macrophages are one of the first cells subjected to the infectious effect of viruses. The present paper analyses for the first time the ultrastructural changes in macrophages caused by an agent of hemorrhagic fever with renal syndrome (HERS)--hantavirus (HV). After a local fusion with the host cell plasmalemma and its adsorption on the cell surface, the HV penetrates through the macrophage membrane. This process occurred without destruction of cell plasmalemma. HV viral particles were observe within the macrophage cytoplasm mostly on the smooth granular endoplasmic reticulum vesicles. Viroplasts were defined in macrophages after a 2 h incubation, with synthesis of viral nucleoproteins and primary covers being observed on the surface of viroplasts. Viral particles left macrophages in the process of budding on the phagocyte surface. Thereby HV, similar to other enveloped viruses, realizes entrance and egress from the target cell without damaging its plasmalemma. This accounts for the viral ability to reproduce in macrophages for a long time without any cytopathological effect. Consequently, in the absence of obvious destruction changes, mononuclear phagocytes can serve as a long-term storage of viruses, and thus being involved in HV dissemination during HERS.