RESUMEN
The effects of Aloe vera on microcirculation and levels of TNF-alpha and IL-6 were investigated in rats after inducing burn. Seventy-two male Wistar Furth rats were equally divided into four groups as follow: controls (CON), untreated burn-wound rats (BURN), normal saline-treated burn-wound rats (BURN-NSS) and Aloe vera-treated burn-wound rats (BURN-ALOE). The animals in each group were equally subdivided into three subgroups for the study on day 3, 7 and 14 post-burn. Dorsal skinfold chamber preparation and intravital fluorescence microscopic technique were performed to examine leukocyte adhesion on postcapillary venules. ELISA techniques were performed to examine serum TNF-alpha and IL-6 levels. It was found that the amount of leukocyte adhesion was significantly reduced in the BURN-ALOE group compared to rats in the BURN group on day 14. Levels of TNF-alpha and IL-6 were also decreased significantly compared to BURN at all three monitored time points. Aloe vera could inhibit the inflammatory process following burn injury, as characterized by the reduction of leukocyte adhesion, as well as those pro-inflammatory cytokines.
Asunto(s)
Aloe/química , Antiinflamatorios no Esteroideos/uso terapéutico , Quemaduras/tratamiento farmacológico , Interleucina-6/sangre , Leucocitos/efectos de los fármacos , Fitoterapia , Extractos Vegetales/uso terapéutico , Factor de Necrosis Tumoral alfa/análisis , Administración Cutánea , Animales , Antiinflamatorios no Esteroideos/administración & dosificación , Quemaduras/sangre , Adhesión Celular/efectos de los fármacos , Evaluación Preclínica de Medicamentos , Masculino , Extractos Vegetales/administración & dosificación , Plantas Medicinales/química , Ratas , Ratas Endogámicas WF , Piel/efectos de los fármacos , Piel/lesionesRESUMEN
The objective of this study was to assess the energy expenditure and physical activity in obese (n=21) and non-obese (n=21) children (aged 9-12 years) in Bangkok. Anthropometric measurements were performed. Energy expenditure and physical activity were determined by indirect calorimetry (based on oxygen consumption) and the heart rate monitoring method. Physical activity index was calculated by the ratio of total energy expenditure (TEE) to sedentary energy expenditure (SEE). The results showed that obese children had significantly greater (p<0.05) body weight, body mass index, relative weight, body fat (%), fat mass except for height, and fat free mass than the non-obese group. The TEE, SEE, and activity energy expenditure (AEE) were higher (p<0.05) in the obese children when compared with the non-obese ones. The mean values of respiratory quotient (RQ) were 0.91+/-0.06 in obese and 0.89+/-0.08 in the non-obese group, respectively, indicating the contribution of carbohydrate substrate to energy production. Both obese and non-obese children were similar in the physical activity index level of 1.48+/-0.17 and 1.51+/-0.22, respectively, which was lower than that recommended by the World Health Organization (1.7). In conclusion, energy expenditure of obese children was higher than non-obese children because of greater body weight. Furthermore, the physical activity of both groups was lower than that recommended by WHO. To prevent obesity in children, programmimg of exercise and activities as well as nutritional education should be emphasized for school children and parents.
Asunto(s)
Metabolismo Basal/fisiología , Metabolismo Energético/fisiología , Ejercicio Físico , Estilo de Vida , Obesidad/fisiopatología , Antropometría , Índice de Masa Corporal , Calorimetría , Niño , Femenino , Frecuencia Cardíaca/fisiología , Humanos , Masculino , Consumo de Oxígeno , Probabilidad , Estudios Prospectivos , Valores de Referencia , Sensibilidad y Especificidad , TailandiaRESUMEN
OBJECTIVE: To demonstrate the microcirculatory and wound healing effects of Aloe vera on induced second degree burn wounds in rats. METHOD: A total of 48 male Wistar rats were equally divided into 4 groups as follows: sham controls, untreated burn-wound rats, those treated with once-daily application of normal saline (NSS) and those treated with once-daily application of lyophilized Aloe vera gel. The animals in each group were equally subdivided into 2 subgroups for the study of cutaneous microcirculation and wound healing on day 7 and 14 after burn. Dorsal skinfold chamber preparation and intravital fluorescence microscopic technique were performed to examine dermal microvascular changes, including arteriolar diameter, postcapillary venular permeability and leukocyte adhesion on postcapillary venules. RESULTS: On day 7, the vasodilation and increased postcapillary venular permeability as encountered in the untreated burn were found to be reduced significantly (p < 0.05) in both the NSS- and Aloe vera-treated groups, but to a greater extent in the latter. Leukocyte adhesion was not different among the untreated, NSS- and Aloe vera-treated groups. On day 14, vasoconstriction occurred after the wound had been left untreated. Only in the Aloe vera-treated groups, was arteriolar diameter increased up to normal condition and postcapillary venular permeability was not different from the sham controls. The amount of leukocyte adhesion was also less observed compared to the untreated and NSS- treated groups. Besides, the healing area of the Aloe vera-treated wound was better than that of the untreated and NSS- treated groups during 7 and 14 days after burn. CONCLUSION: Aloe vera could exhibit the actions of both anti-inflammation and wound healing promotion when applied on a second degree burn wound.