RESUMEN
Marketed formulations of erythropoietin (EPO) ior®EPOCIM, MIRCERA® and two newly developed pegylated-EPO analogues (PEG-EPO 32 and 40â¯kDa) formulations were intravenously administered to New Zealand rabbits. A semi-mechanistic Pharmacokinetic/Pharmacodynamic (PK/PD) model describing in a simultaneous and integrated form the time course of reticulocytes, red blood cells and hemoglobin was built to account for the time course of hematopoiesis stimulation after erythropoietin administration. Data analysis was performed based on the population approach with the software NONMEM version 7.3. Erythropoietin disposition of each of the administered formulations was best described with a two compartment model and linear elimination. Different formulations show different clearance and apparent volume of distribution of the central compartment but share estimates of inter-compartmental clearance and apparent peripheral volume of distribution. A semi-mechanistic model including cell proliferation, maturation, and homeostatic regulation provided a good description of the data regardless the type of erythropoietin formulation administered. The system-, and drug-related parameters showed consistency and differed across formulations, respectively. A single IV administration of PEG-EPO 32 and 40â¯kDa formulations in New Zealand rabbits achieves a median change of 27% and 22% on RET levels, and of 47% and 63% on RBC and HGB levels, respectively compared to MIRCERA®. The administration of new branched PEG-chains formulations improves PK and PD properties of EPO, in terms of increasing elimination half-lives and pharmacological activity on RET, RBC and HGB compared to commercially available formulations (ior®EPOCIM and MIRCERA®).
Asunto(s)
Eritropoyetina/farmacocinética , Hematínicos/farmacocinética , Hematopoyesis/efectos de los fármacos , Modelos Biológicos , Polietilenglicoles/farmacocinética , Animales , Disponibilidad Biológica , Composición de Medicamentos , Eritrocitos/efectos de los fármacos , Eritrocitos/metabolismo , Eritropoyetina/administración & dosificación , Eritropoyetina/sangre , Eritropoyetina/química , Hematínicos/administración & dosificación , Hematínicos/sangre , Hematínicos/química , Hemoglobinas/metabolismo , Inyecciones Intravenosas , Modelos Lineales , Masculino , Polietilenglicoles/administración & dosificación , Polietilenglicoles/química , Conejos , Proteínas Recombinantes/farmacocinética , Reticulocitos/efectos de los fármacos , Reticulocitos/metabolismoRESUMEN
Numerous immunochemical methods are now available for the detection of antibodies to gangliosides. An amplified ELISA method for detection of autoantibodies to NGcGM3 ganglioside in the sera of patients with various type of renal diseases was developed. IgM antibodies were found in 39 out of 53 sera of patients using 30 normal healthy blood donor as a negative control. For human IgG conjugate no reactivity to NGcGM3 was seen in the sera. Positive ELISA results were confirmed by TLC-immunostaining using GM3, NGcGM3, NGcGM2 and Standard bovine gangliosides (GM1, GD1a, GD1b and GT1b). All sera were also assayed for reactivity with GM3 in ELISA to determine the line specificity of these antibodies. Based on these results, a protocol for a sensitive and reproducible amplification ELISA system for serum anti-NGcGM3 antibodies in patients with renal or other diseases is presented. The ELISA method described here in appear to be useful adjunt to measure antiNGcGM3 antibodies in sera of patients with various type of renal or other diseases.
Asunto(s)
Autoanticuerpos/sangre , Ensayo de Inmunoadsorción Enzimática/métodos , Gangliósido G(M3)/análogos & derivados , Cromatografía en Capa Delgada , Gangliósido G(M3)/inmunología , Humanos , Reproducibilidad de los ResultadosRESUMEN
A program is described for symmetric parallel line analysis of bioassay data with a logistic dose-response relationship. Dose-response relationship is transformed to semilog or log-log. A regression line can be calculated for the dose-response curve for standard and test samples, and produces potency estimate of test sample preparation relative to the standard preparation based on parallel-line bioassay statistical methods [3,4], together with detailed analysis of variance, estimates of slope, intercept and chi 2 test which permits a very sensitive test for linearity and parallelism of standard and test sample and facilitates detection of 'outliers', i.e. samples exhibiting non-parallelism. The general comparison of dose-response relationships produced by the program are a feature of particular interest.