RESUMEN
Genomes of some positive-strand RNA viruses do not contain cap-structure, but instead their 5'-end is covalently linked to a viral protein called VPg. Complex formation between VPg and cellular translation initiation factors (eIFs) has been extensively studied in the context of the model of this complex involvement in virus mRNA translation initiation and cellular protein translation shut down in infected cells. The potato virus (PVY) VPg was expressed in bacterial and baculovirus systems in order to investigate its binding capacity to wheat eIF4E and its isoform. Both purified recombinant eIF4E and eIF(iso)4E were identified in vitro as binding partners of the purified recombinant VPg by using affinity chromatography, as well in vivo by coexpressing of recombinant VPg and eIFs in insect cells with following complex purification using affinity chromatography. Besides it was shown that PVY VPg also formed a complex with endogenous insect eIF4E in vivo. PVY VPg interaction with eIF4E of wheat (non permissive plant for PVY), and also with so evolutionary distant partner as insect eIF4E suggests the conservation of general structural features of eIF4E implicated in the formation of the complex with VPg.
Asunto(s)
Factor 4E Eucariótico de Iniciación/genética , Genoma Viral , Potyvirus/genética , Proteínas Recombinantes/genética , Ribonucleoproteínas/genética , Proteínas no Estructurales Virales/genética , Animales , Baculoviridae/genética , Baculoviridae/metabolismo , Cromatografía de Afinidad , Escherichia coli/genética , Escherichia coli/metabolismo , Plásmidos , Potyvirus/metabolismo , Unión Proteica , Solanum tuberosum/metabolismo , Solanum tuberosum/virología , Spodoptera/metabolismo , Spodoptera/virología , Triticum/metabolismoAsunto(s)
Anastomosis Quirúrgica , Motilidad Gastrointestinal/fisiología , Complicaciones Posoperatorias/fisiopatología , Complicaciones Posoperatorias/terapia , Úlcera Gástrica/cirugía , Infección de la Herida Quirúrgica/terapia , Terapia Combinada , Humanos , Complicaciones Posoperatorias/prevención & control , Infección de la Herida Quirúrgica/etiología , Infección de la Herida Quirúrgica/prevención & controlRESUMEN
Human prolactin was expressed in insect culture cells by recombinant baculoviruses carrying prolactin gene cDNA placed under the transcriptional control of polyhedrin gene promoter of Autographa californica nuclear polyhedrosis virus. Preliminary results of recombinant human prolactin expression as extracellular as well as intracellular product of baculovirus expression system were presented at the FEBS Meeting in Nice, France, in 1999 (Abstracts, p. 288). In the present work prolactin was expressed as a hexahistidine-tagged fusion protein and recombinant protein was purified by metal affinity resin. Yields varied between approximately 20 and 35 mg/liter of medium. This recombinant prolactin was biologically active in Nb2 lymphoma cell proliferation assay and after simple purification could substitute for pituitary-derived prolactin.
Asunto(s)
Líquido Intracelular/metabolismo , Prolactina/biosíntesis , Prolactina/genética , Spodoptera/genética , Spodoptera/metabolismo , Animales , Baculoviridae/genética , Bioensayo , Sistema Libre de Células/química , Sistema Libre de Células/metabolismo , Células Cultivadas , Clonación Molecular , Sustancias de Crecimiento/biosíntesis , Sustancias de Crecimiento/genética , Sustancias de Crecimiento/aislamiento & purificación , Sustancias de Crecimiento/fisiología , Humanos , Cuerpos de Inclusión/genética , Cuerpos de Inclusión/metabolismo , Peso Molecular , Nucleopoliedrovirus/genética , Prolactina/aislamiento & purificación , Prolactina/fisiología , Desnaturalización Proteica , Renaturación de Proteína , Ratas , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , Proteínas Recombinantes/farmacología , Solubilidad , Spodoptera/citología , Spodoptera/virología , Células Tumorales CultivadasRESUMEN
The one-layer intestinal suture, using microsurgical technique, was done in experiment on 68 laboratory animals (157 small intestinal and colonic anastomoses were formed in total). Maximally favourable conditions, promoting the primary closure of the anastomoses healed were established. The proposed anastomosing method efficacy was confirmed by favourable results of operative interventions, performed in 108 patients with different surgical diseases in the planned and urgent order.
Asunto(s)
Procedimientos Quirúrgicos del Sistema Digestivo/métodos , Intestinos/cirugía , Microcirugia/métodos , Estómago/cirugía , Absorción , Anastomosis Quirúrgica/métodos , Animales , Perros , Conejos , Dehiscencia de la Herida Operatoria/prevención & control , Técnicas de Sutura , SuturasRESUMEN
Comparative estimation of the native microsurgical atraumatic suture material metallatraum and the foreign threads (prolene and virgin silk) application while the biliobiliary and biliodigestive anastomoses formation was done in experiment. Most favourable tissue reaction was noted using monolithic threads prolene and metallatraum. Metallatraum thread is the most resistant one to the calculi deposition on it. The silk thread application in biliary ducts surgery is not indicated.
Asunto(s)
Enfermedades de las Vías Biliares/prevención & control , Procedimientos Quirúrgicos del Sistema Biliar/instrumentación , Procedimientos Quirúrgicos del Sistema Biliar/métodos , Cálculos/prevención & control , Complicaciones Intraoperatorias/prevención & control , Portoenterostomía Hepática/instrumentación , Portoenterostomía Hepática/métodos , Suturas , Humanos , Microcirugia/instrumentación , Microcirugia/métodosRESUMEN
Intestinal and gastro-intestinal anastomoses, performed using synthetic absorbable suture materials, foreign-dexon, vicryl and polydioxanon and native-oktselon, were studied up in experiment. The oktselon thread do not cede to foreign threads in quality and has advantages over them in some features. It is possible to regulate the terms of a firmness loss and resorption changing the threads oxidation degree during their manufacturing. In tissues, sutured using oktselon thread, the processes of reparative regeneration are passing in maximally favourite conditions.
Asunto(s)
Gastroenterostomía/métodos , Intestinos/cirugía , Poliésteres , Estómago/cirugía , Suturas , Animales , Materiales Biocompatibles , Biodegradación Ambiental , Perros , ConejosRESUMEN
The translational activity of embryos cultured up to the 8-cell stage in cytochalasin D-supplemented medium was studied. These embryos remained unicellular during the entire preimplantation period. Blastocoel formation and hatching began in the cytochalasin-treated embryos at the same time as in the control. We have studied the overall translation rates of "one-cell embryos" and estimated the relative translational differences for individual polypeptides compared to the control embryos. Up to the early blastocyst stage, the translation levels in these embryos, measured by 35S-methionine incorporation, were about two times lower than in the control. At later stages, the differences were fivefold. The ppm values of approximately one-third of all individual spots on two-dimensional electrophoregrams differed three times or more between the control and cytochalasin-treated embryos. These results suggest that the first morphogenetic events in mammalian ontogenesis may be controlled autonomously and the timing of morphogenetic transitions is controlled by acquisition of a definite set of stage-specific factors which serves as the signal for subsequent development.
Asunto(s)
Blastocisto/citología , Biosíntesis de Proteínas/fisiología , Animales , Blastocisto/química , Blastocisto/efectos de los fármacos , División Celular/efectos de los fármacos , Técnicas de Cultivo/métodos , Citocalasina D/farmacología , Depresión Química , Electroforesis en Gel Bidimensional/métodos , Ratones , Ratones Endogámicos , Inhibidores de la Síntesis del Ácido Nucleico/farmacología , Biosíntesis de Proteínas/efectos de los fármacosRESUMEN
It is found out that the acceleration of preimplantation morphogenesis is observed in mouse chimeras between single two-cell-stage blastomeres of BALB/c and [BALB/c x C57B1/6] x C57B1/6 (F2) embryos. The paired aggregation of blastomeres of two-cell stage made F2-BALB/c chimeras to start cavitation 8 +/- 2 hours earlier than F2<-->F2 and BALB/c-BALB/c control embryos. We have demonstrated for the first time the 'chimeric' heterosis on preimplantation stages. Possible approaches to the future studies of this phenomenon are discussed.
Asunto(s)
Quimera/fisiología , Cruzamientos Genéticos , Ratones Endogámicos BALB C/embriología , Ratones Endogámicos C57BL/embriología , Animales , Blastocisto/fisiología , Femenino , Vigor Híbrido/fisiología , Masculino , Ratones , Morfogénesis/fisiología , Factores de TiempoRESUMEN
During ten-year period 1191 patients with perforative pyloroduodenal ulcer were operated on. Palliative intervention was conducted in 206 patients, conventionally radical one--in 677 and radical--in 308. Postoperative mortality have constituted 5.2%.
Asunto(s)
Úlcera Duodenal/complicaciones , Úlcera Péptica Perforada/cirugía , Enfermedad Crónica , Úlcera Duodenal/mortalidad , Úlcera Duodenal/cirugía , Duodeno/cirugía , Femenino , Humanos , Masculino , Cuidados Paliativos , Úlcera Péptica Perforada/mortalidad , Píloro/cirugíaAsunto(s)
Úlcera Duodenal/complicaciones , Úlcera Péptica Perforada/cirugía , Cuidados Preoperatorios/métodos , Choque/cirugía , Úlcera Gástrica/complicaciones , Anciano , Terapia Combinada , Úlcera Duodenal/cirugía , Femenino , Humanos , Masculino , Persona de Mediana Edad , Úlcera Gástrica/cirugíaRESUMEN
Low viability of manipulated or in vitro cultured is caused primarily by the reduced cell number in the implanting blastocysts. In order to investigate the effect of implantation delay on embryo viability and cell number, mouse blastocysts were transferred into oviducts of day 0 pseudopregnant females. This type of transfer improved embryo survival rates, indicating that embryos retarded by in vitro culture restored their viability during 3 days of delayed implantation. Our results showed that even in the cases when the initial cell count was as low as 28.2 +/- 0.7 cells per blastocyst (vs 60.5 +/- 1.4 cells in the control blastocysts, developed in vivo), implantation delay increased this number to 107.2 +/- 3.5 cells (control blastocysts had at this stage on average 111.0 +/- 3.7 cells). Half-blastocysts, developed from the single blastomeres of the 2-cell embryos or from experimentally produced tetraploids, had around 50 cells after 3 days of implantation delay. This indicates that the start of blastocyst dormancy is triggered during the eighth cell cycle and independent of the absolute cell number or the number cytokineses. Implantation-delayed blastocysts, developed from the half-embryos with the doubled volume of cytoplasm, had on average 70.5 +/- 2.4 cells, suggesting that embryo fall into quiescence is also dependent upon the attainment of a definite nucleo-cytoplasmic ratio. We conclude that blastocyst readiness for implantation is determined by two factors: number of cell cycles and nucleo-cytoplasmic ratio.
Asunto(s)
Blastocisto/citología , Blastocisto/fisiología , Animales , Supervivencia Celular , Implantación del Embrión , Trompas Uterinas , Femenino , Masculino , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Ratones Endogámicos ICR , Mórula/citología , Mórula/fisiología , Embarazo , Seudoembarazo , Superovulación , Factores de TiempoRESUMEN
The results of the creation of new expressive system on Malacosoma neustria nuclear polyhedrosis virus and cell line originated from Antheraea pernyi tissues was demonstrated. The expression of bacterial beta-galactosidase-coding gene was analyzed in this system.
Asunto(s)
Regulación Viral de la Expresión Génica/genética , Vectores Genéticos/genética , Mariposas Nocturnas/genética , Nucleopoliedrovirus/genética , Animales , Secuencia de Bases , Células Cultivadas , Clonación Molecular , ADN Viral/genética , ADN Viral/aislamiento & purificación , Escherichia coli/genética , Datos de Secuencia Molecular , Mariposas Nocturnas/virología , Plásmidos/genética , Regiones Promotoras Genéticas/genética , beta-Galactosidasa/genéticaRESUMEN
The effects of low doses of environmental ionizing radiation on reproductive functions of seven generations of CC57W/Mv mice from Chernobyl experimental population has been investigated. A decrease in the number of litters per female during reproductive period, the number of newborn animals per litter, and an increase in the postnatal mortality during the first month of life were shown. These effects are due to the stable disorders of parental reproductive system and the high level of in-vivo preimplantation deaths in F1--F7 generations of CC57W/Mv mice from the Chernobyl experimental populations.
Asunto(s)
Ratones Endogámicos C57BL/fisiología , Centrales Eléctricas , Liberación de Radiactividad Peligrosa , Reproducción/efectos de la radiación , Animales , Relación Dosis-Respuesta en la Radiación , Femenino , Muerte Fetal/etiología , Genoma , Tamaño de la Camada/efectos de la radiación , Masculino , Ratones , Ratones Endogámicos C57BL/genética , Factores de Tiempo , UcraniaAsunto(s)
Adaptación Fisiológica , Altitud , Ritmo Circadiano/fisiología , Electroencefalografía , HumanosRESUMEN
Data on individual biorhythms are presented for 270 children aged 7-17 living in the regions situated at 760, 1700 and 2850 m above the sea level. Central hemodynamics was measured by Kubicek et al. method 6 times a day every 6 hours. The analysis of the pilot evidence was conducted by cosinor program, then an original method was introduced which allowed evaluation of the degree of biorhythmologic dispersion in the samples. The findings indicate a significant increase in hemodynamic biorhythm polymorphism and broadening of "biorhythmological norm" in children population aged 7-17 under the middle and high mountain conditions.
Asunto(s)
Altitud , Circulación Sanguínea , Periodicidad , Adolescente , Niño , Hemodinámica , Humanos , KirguistánRESUMEN
The results of studying the daily rhythmicity of orthostatic tolerance of blood system in practically healthy men are presented. The orthostatic test was performed 6 times a day with a 4-hour interval. The parameters of systemic, cerebral and peripheric hemodynamics have been recorded. It is found that a degree of a change in an intensity of cerebral blood supply experiences the marked variations depending on time of day. The day-night cycling of orthostatic tolerance of cerebral circulation is greatly determined by the biorhythm of the responses of arterial vessels of the lower extremities, i.e., by the natural periodization of blood redistribution processes. The highest orthostatic tolerance is noted at 3 to 11 a.m. and the lowest one at 3-7 p.m.
Asunto(s)
Ritmo Circadiano/fisiología , Postura/fisiología , Adolescente , Adulto , Circulación Cerebrovascular/fisiología , Hemodinámica/fisiología , Humanos , Masculino , Factores de TiempoRESUMEN
The results of a comparative experiment for studying the native glue composition on a fibrin base, which possesses the hemostatic and adhesive properties and has the influence on the processes of regeneration, and modern synthetic medical glues are presented. The essential advantages of a fibrin-based glue were revealed.