RESUMEN
Seven genetic variants of Yersinia pestis were detected by finger-printing of 85 strains of this bacterium from natural foci by means of a BX probe. Variants of Y. pestis strains correlate with certain species of carriers.
Asunto(s)
Reservorios de Enfermedades , Yersinia pestis/genética , Dermatoglifia del ADN , ADN Bacteriano/genética , Genotipo , Especificidad de la EspecieRESUMEN
Y. pestis high repeated sequences DNA (HRS) used as probes in the blot hybridization procedure made it possible to reveal some tendencies of their location on the chromosome, namely, the correlation with the regions where Y. pestis strains were isolated, correlation with the stability of their properties and, perhaps, with the variation of individual strains.
Asunto(s)
ADN Bacteriano/genética , Secuencias Repetitivas de Ácidos Nucleicos , Yersinia pestis/genética , Dermatoglifia del ADNRESUMEN
The authors have developed the optimal variant of the enzyme immunoassay system that contains plague murine monoclonal and equine polyclonal immunoglobulins and identified all plague microbe strains at a high sensitivity (3.84 m.cl/ml). The cross reactivity of monoclonal antibodies with some pseudotuberculosis agent strains does not prevent from using the developed test system for laboratory diagnosis of plague, particularly while isolating atypical (capsule- or plasmid-free) variants of Y. pestis, taking into account parallel special diagnostic tests for pseudotuberculosis.
Asunto(s)
Técnicas para Inmunoenzimas/instrumentación , Yersinia pestis/inmunología , Animales , Anticuerpos Monoclonales/inmunología , Especificidad de Anticuerpos , Reacciones Cruzadas , Caballos , Hibridomas/inmunología , Inmunoglobulinas/inmunología , Lipopolisacáridos/inmunología , Ratones , Plásmidos/inmunología , Sensibilidad y Especificidad , Yersinia pseudotuberculosis/inmunologíaRESUMEN
The study of the plasmid composition of 246 Y. pestis strains from different natural foci in the USSR and other countries revealed that 173 strains (70%) carried three known plasmids with a molecular weight of about 6, 45-50 and 60 megadaltons (MD) respectively. In 20 strains (8%) obtained from different sources additional cryptic plasmids were detected. In some cases the absence of one or two typical plasmids was observed. Replicon pPst was shown to have quite constant molecular weight (6 MD), whereas plasmids pCad and especially pFra exhibited certain variations of their molecular weight (45-49 MD and 60-149 MD respectively) in strains of different origin.
Asunto(s)
Reservorios de Enfermedades , Plásmidos , Yersinia pestis/genética , Electroforesis en Gel de Agar , Genes Bacterianos , Especificidad de la EspecieRESUMEN
Plasmid content in 242 Yersinia pestis strains from various natural plague foci of the U.S.S.R. and other countries was studied. Of these strains, 172 (71%) were shown to carry three plasmids described previously of about 6, 45-50 and 60 MDa, respectively. Twenty strains (8%) from different foci harboured additional cryptic plasmids, most often of about 20 mDa in size. Plasmid pPst displayed considerable constancy of its molecular mass. On the contrary, size variations of pCad (45-49 MDa) and, especially, pFra (60-190 MDa) were found. Molecular mass of these plasmids correlated with the host strain origin.