RESUMEN
AIM: To analyze neurological complications and outcomes of bacterial meningitis (BM) in children. MATERIAL AND METHODS: Fifty-five patients with BM, aged from 2 months to 12 years, were examined. Bacteriological study, clinical and biochemical blood tests and blood serology and cerebrospinal fluid (CSF) tests as well as serum molecular-genetic study were performed. Neuroimaging methods (neurosonography, computed (X-ray) tomography and brain magnetic resonance imaging) were used. RESULTS AND СONCLUSION: A key role of generalized meningococcal infection in the development of BM in children was confirmed. Brain edema was an early and life-threatening complication of BM. It was found in 9% of the patients with meningococcal infection, 7.3% with pneumococcal meningitis and 3.6% with haemophilus meningitis. Changes in the brain structure were not found in 80% of the patients, in 20%, the residual stage of BM was characterized by cerebral destructive/proliferative or atrophic changes of different severity. The dissociation between clinical and neuroimaging parameters and poor outcomes of BM, related to the complicated premorbid state, concomitant somatic/neurological pathology, BM severity, late laboratory diagnosis and untimely etiotropic antibacterial treatment, were identified in 65.5%.
Asunto(s)
Antibacterianos/uso terapéutico , Meningitis Bacterianas , Meningitis por Haemophilus , Meningitis Neumocócica , Encéfalo , Niño , Humanos , Imagen por Resonancia Magnética , Meningitis Bacterianas/complicaciones , Meningitis Bacterianas/diagnóstico , Meningitis Bacterianas/tratamiento farmacológico , Meningitis Neumocócica/complicaciones , Meningitis Neumocócica/diagnóstico , Meningitis Neumocócica/tratamiento farmacológicoRESUMEN
AIM: To improve clinical/laboratory diagnosis of bacterial meningitis (BM) in children. MATERIAL AND METHODS: Fifty-seven patients, aged from 4 months to 12 years, with BM were examined. Bacteriological study of the mucus from the nasopharynx, sterility tests of blood and cerebrospinal fluid (CNF), clinical and biochemical tests, serological study of paired samples of plasma - indirect hemagglutination test, latex agglutination assay, immunological study of plasma and CNF with the determination of interferon -α, -ß and -γ, interferon procalcitonin and neopterin with ELISA were performed at admission and after CNF remediation. Molecular-genetic study of the blood serum and CNF using PCR, neurosonography, computed tomography and MRI of the brain were done as well. RESULTS AND CONCLUSION: Most of the patients had generalized meningococcus infection (64,9%). Hib-meningitis, pneumococcus meningitis and other forms were less frequent. Characteristics of neurological presentations of BM depending on the etiology, somatic complications and time from the manifestation of symptoms were described. Clinical/biochemical criteria of BM and correlations between procalcitonin, neopterin and interleukin-8 in the CNF and blood of the patients and disease severity as well as between the level of inflammatory reactions in the CNF and the BM form were established. These results are important for the development of informative biomarkers for the prognosis of BM course and outcome.
Asunto(s)
Meningitis Bacterianas/diagnóstico , Meningitis Bacterianas/terapia , Bacterias/aislamiento & purificación , Biomarcadores/análisis , Biomarcadores/sangre , Biomarcadores/líquido cefalorraquídeo , Calcitonina/análisis , Calcitonina/sangre , Calcitonina/líquido cefalorraquídeo , Niño , Preescolar , Femenino , Humanos , Lactante , Interleucina-8/análisis , Interleucina-8/sangre , Interleucina-8/líquido cefalorraquídeo , Masculino , Meningitis Bacterianas/sangre , Meningitis Bacterianas/líquido cefalorraquídeo , Faringe/microbiología , PronósticoRESUMEN
Stability of levorin isolated and purified with the use of ionole phosphorus organic analogs having in their structure phosphate, phosphonate and phosphonite groups was studied. The compound having in its structure (in addition to tertiary butyl substitutes) the phosphonite group with the P-H bond increasing the compound antioxidant property was shown to have the highest stabilizing effect. The results of the study made it possible to recommend the use of the space complicated phenols with the structure fragments of the P-H bond type as antioxidants in production of levorin.
Asunto(s)
Antifúngicos/síntesis química , Hidroxitolueno Butilado/análogos & derivados , Candicidina/síntesis química , Antifúngicos/farmacología , Antioxidantes/química , Candicidina/química , Candicidina/farmacología , Candida/efectos de los fármacos , Estabilidad de Medicamentos , Pruebas de Sensibilidad Microbiana , Fósforo/química , Relación Estructura-ActividadRESUMEN
The authors studied the membrane-stabilizing effect of two water-soluble preparations of antioxidants, analogues of ionol, in thermal burns. It was found that both preparations increase somewhat to a different degree the resistance of the red cell membranes to peroxide compounds and reduce hemolysis which is very characteristic of the first minutes and hours after the thermal trauma. The preparations alter the electrical properties of the red cells and affect the activity of phospholipases in blood plasma.
Asunto(s)
Quemaduras/tratamiento farmacológico , Hidroxitolueno Butilado/uso terapéutico , Fósforo , Agua/química , Animales , Hidroxitolueno Butilado/química , Masculino , Fósforo/análisis , Ratas , SolubilidadRESUMEN
Four mouse monoclonal antibodies (E11, A8, F8, H5) to alpha-endorphin have been produced. The antibodies bind 12.5, 20.6, 9.6, 6.6% of 125I-beta-endorphin and 35.5, 15.1, 12.8, 12.2% of 125I-gamma-endorphin; the binding of 125I-alpha-endorphin being taken for 100%. The binding of antibodies E11, A8, F8 and H5 to 125I-alpha-endorphin was 50% inhibited by unlabeled ligand in concentrations 5, 50, 30 and 35 nM respectively. Using tissue sections of rat pituitary it was shown that antibody E11 can be used for the localization of endorphin producing cells by immunofluorescence. The antibodies F8 and H5 effectively detected endorphin precursor proopiomelanocortin by immunoblotting.