RESUMEN
Cobalt ferrite nanoparticles (NPs) have received increasing attention due to their widespread therapeutic and agricultural applicability. In the environmental field, dry powder- and ferrofluid-suspended cobalt ferrite NPs were found to be useful for removing heavy metals and metalloids from water, while diluted suspensions of cobalt ferrite NP have been promisingly applied in medicine. However, the potential toxicological implications of widespread exposure are still unknown. Since cobalt ferrite NPs are considered residual wastes of environmental or medical applications, plants may serve as a point-of-entry for engineered nanomaterials as a result of consumption of these plants. Thus, the aim of this study was to assess the effects of dry powder and fresh cobalt ferrite NP on wheat plants. Seven-day assays were conducted, using quartz sand as the plant growth substrate. The toxicity end points measured were seed germination, root and shoot lengths, total cobalt (Co) and iron (Fe) accumulation, photosynthetic pigment production, protein (PRT) production, and activities of catalase (CAT), ascorbate peroxidase (APX), and guaiacol peroxidase (GPX). Increasing total Co and Fe in plant tissues indicated that wheat plants were exposed to cobalt ferrite NP. Seed germination and shoot length were not sufficiently sensitive toxicity end points. The effective concentration (EC50) that diminished root length of plants by 50% was 1963 mg/kg for fresh ferrite NPs and 5023 mg/kg for powder ferrite NP. Hence, fresh ferrite NPs were more toxic than powder NP. Plant stress was indicated by a significant decrease in photosynthetic pigments. CAT, APX, and GPX antioxidant enzymatic activity suggested the generation of reactive oxygen species and oxidative damage induced by cobalt ferrite NP. More studies are thus necessary to determine whether the benefits of using these NPs outweigh the risks.
Asunto(s)
Cobalto/toxicidad , Compuestos Férricos/toxicidad , Estrés Oxidativo , Fotosíntesis/efectos de los fármacos , Triticum/efectos de los fármacos , Antioxidantes/metabolismo , Cobalto/química , Compuestos Férricos/química , Germinación/efectos de los fármacos , Nanopartículas del Metal , Pigmentos Biológicos/metabolismo , Proteínas de Plantas/biosíntesis , Polvos/química , Polvos/toxicidad , Plantones/crecimiento & desarrollo , Semillas/efectos de los fármacos , Semillas/enzimología , Semillas/crecimiento & desarrollo , Semillas/fisiología , Pruebas de Toxicidad , Triticum/enzimología , Triticum/crecimiento & desarrollo , Triticum/fisiologíaRESUMEN
The aim of this work was to assess the uptake of citrate-coated magnetite nanoparticles (NPs) by wheat plants and its effect on the bioaccumulation and toxicity of individual and joint Cd(2+) and Cr(6+) levels. Seven-day assays were conducted using quartz sand as the plant growth substrate. The endpoints measured were seed germination, root and shoot lengths, and heavy metal accumulation. Magnetite exhibited very low toxicity, regardless of the wheat seedling NP uptake and distribution into roots and shoots. The seed germination and shoot length were not sensitive enough, while the root length was a more sensitive toxicity endpoint. The root length of wheat seedlings exposed to individual metals decreased by 50% at 2.67mgCd(2)(+)kg(-1) and 5.53mgCr(6+)kg(-1). However, when magnetite NPs (1000mgkg(-1)) were added, the root length of the plants increased by 25 and 50%. Cd(2+) and Cr(6+) showed similar and noninteractive joint action, but strongly impaired the wheat seedlings. In contrast, an interactive infra-additive or antagonistic effect was observed upon adding magnetite NPs. Thus, cadmium and chromium accumulation in vegetable tissues was considerately diminished and the toxicity alleviated.
Asunto(s)
Cadmio/toxicidad , Cromo/toxicidad , Nanopartículas de Magnetita/toxicidad , Contaminantes del Suelo/toxicidad , Triticum/efectos de los fármacos , Ácido Cítrico/química , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/metabolismo , Brotes de la Planta/efectos de los fármacos , Brotes de la Planta/crecimiento & desarrollo , Brotes de la Planta/metabolismo , Plantones/efectos de los fármacos , Plantones/crecimiento & desarrollo , Triticum/crecimiento & desarrollo , Triticum/metabolismoRESUMEN
Phytoremediation is a technology for extracting or inactivating pollutants. Echinochloa polystachya [(H.B.K.) Hitchcock] (Poaceae) is a fast-growing perennial grass that is common in tropical areas and is often found in oil-polluted soils that contain high concentrations of heavy metals. However, its tolerance to heavy metals, and its ability to accumulate them, has yet to be investigated. Here we test the hypothesis that E. polystachya is able to accumulate high concentrations of cadmium (Cd). Plants were grown hydroponically with different levels of Cd(2+) (0, 0.25, 1, 2, 10, 50, and 100mgL(-1)), and were found to be tolerant to Cd(2+) at all levels. No metal-toxicity symptoms were observed at any Cd(2+) level. Root and leaves Cd concentrations were 299+/-13.93 and 233+/-8.77mgkg(-1) (on a dry weight basis), respectively. Scanning electron microscopy showed the inclusion of Cd within the xylem; this result was confirmed by energy dispersive X-ray spectrometry. Leaf tissues also accumulated Cd, especially within the bulliform cells of the epidermis. We conclude that E. polystachya is a hyperaccumulator of Cd. While data for other metals are not yet available, E. polystachya shows promise in the phytoextraction of Cd from polluted tropical sites.