RESUMEN
UNLABELLED: PADMA 28, a natural herbal multi-compound remedy originates from traditional Tibetan medicine and possesses a variety of beneficial effects on experimental and clinical models of inflammation and atherosclerosis, as well as angioprotecive, antioxidative and wound-healing properties. The aim of the present study was to evaluate the in vivo influence of this remedy on the in vitro mitogen-induced proliferation of murine splenic lymphocytes and their chemokinetic activity in cell culture.The study was performed on 6-8 weeks old inbred Balb/c mice. PADMA28 was administered to mice per os in daily doses 5.8 mg (calculated from the highest dose recommended for human) or 0.085 mg (dose from the range of active doses of other herbal extracts containing polyphenolic substances used previously by us in experiments with mice), for 7 days. Control groups received water. RESULTS: No substantial differences were observed between groups of mice fed with low and high PADMA doses. In both of them, response of splenic lymphocztes to mitogen PHA (p < 0.001) and their in vitro chemokinetic activity (p < 0.001 for low dose and p < 0.01 for high dose) were highly significantly increased as compared to the controls. CONCLUSION: The results of our investigations suggest that PADMA 28 can stimulate cell-mediated immunity in mice and might be used for this purpose in the wide spectrum of doses.
Asunto(s)
Linfocitos/citología , Linfocitos/efectos de los fármacos , Mitógenos/toxicidad , Extractos Vegetales/farmacología , Bazo/citología , Animales , Movimiento Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Relación Dosis-Respuesta a Droga , RatonesRESUMEN
Echinacea purpurea (EP) and Echinacea angustifolia (EA) are ones of the most important world's herbs with immunotropic activity. They were traditional medicinal plants used by North American Indians for the treatment of various illnesses. Now they are cultivated in many countries and are used mainly to treat respiratory tract infections. Rhodiola rosea (RR) and Rhodiola quadrifida (RQ) are medicinal plants originated from Asia and used traditionally as adaptogens, antidepressants, and anti-inflammatory remedies. We previously reported, that extracts of underground parts of RR and RQ exhibited immunotropic activity. We have demonstrated in pigs that in vitro RR or RQ supplementation of blood lymphocyte cultures stimulated T cell proliferative response to Con A in lower, and inhibited it in higher Rhodiola extract concentrations. The aim of this work was to evaluate the in vivo effect of these herbal remedies on the in vitro proliferative response of mouse splenic lymphocytes to another T-cell mitogen- Phaseolus vulgaris haemagglutinin (PHA). We have found significant stimulation of proliferative response, in comparison to the controls, in mice fed lower doses of tested remedies, and inhibition, no effect or lower stimulation, in mice fed higher doses of these drugs.
Asunto(s)
Echinacea/química , Linfocitos/efectos de los fármacos , Mitógenos/toxicidad , Extractos Vegetales/farmacología , Rhodiola/química , Animales , Proliferación Celular , Relación Dosis-Respuesta a Droga , Femenino , Linfocitos/citología , Linfocitos/fisiología , Ratones , Ratones Endogámicos BALB C , Extractos Vegetales/química , Bazo/citologíaRESUMEN
The immunomodulatory activity of acetylshikonin (ACS) and isobutyrylshikonin (IBS) was studied in female and male inbred Balb/c mice, and in F1 hybrids (Balb/c x C3H). ACS and IBS were isolated from Lithospermum canescens Lehm. (Boraginaceae) roots. Splenocytes from mice fed 40 microg of ACS had higher proliferative potential in cultures with PHA than corresponding controls and also higher migratory in vitro activity than splenocytes obtained from control animals. ACS at a 40 microg daily dose stimulated G-v-H reaction but inhibited it at a 200 microg dose. IBS at a 40 microg dose significantly increased humoral response.
Asunto(s)
Adyuvantes Inmunológicos , Formación de Anticuerpos/efectos de los fármacos , Inmunidad Celular/efectos de los fármacos , Lithospermum/química , Naftoquinonas/farmacología , Animales , División Celular/efectos de los fármacos , Eritrocitos/inmunología , Femenino , Reacción Injerto-Huésped , Masculino , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C3H , Raíces de Plantas/química , Ovinos/inmunología , Bazo/citología , Bazo/inmunologíaRESUMEN
One of the major functions of the immune system is anti-bacterial defence mediated among others by non-specific immunity (macrophages, granulocytes). Echinacea purpurea extracts are widely used in prophylaxis and therapy of various infections, mainly the respiratory tract, in animals and humans. The aim of this work was to evaluate the effect of prophylactic use of Echinacea purpurea extract on the development of Pseudomonas aeruginosa infection in various strains of mice and on some parameters of non-specific and also specific cellular immunity. Mice expressed various, depending on the strain used, susceptibility to infection. Echinacea feeding resulted in diminishing of bacteria number in livers of C57Bl/6 (susceptible strain) as well as B6C3F1 (relative resistant strain) mice. Echinacea feeding of the second relative resistant strain (BALB/c x C3H) F1 resulted in stimulation of granulocytes chemiluminescent and lymphocytes proliferative response.
Asunto(s)
Adyuvantes Inmunológicos/farmacología , Echinacea , Sistema Inmunológico/efectos de los fármacos , Fitoterapia , Extractos Vegetales/farmacología , Infecciones por Pseudomonas/inmunología , Adyuvantes Inmunológicos/administración & dosificación , Administración Oral , Animales , Granulocitos/efectos de los fármacos , Inmunidad Celular/efectos de los fármacos , Hígado/citología , Hígado/microbiología , Linfocitos/efectos de los fármacos , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos , Extractos Vegetales/administración & dosificación , Pseudomonas aeruginosa/clasificación , Pseudomonas aeruginosa/inmunología , Pseudomonas aeruginosa/patogenicidadRESUMEN
Investigations on immune suppression and reconstitution of immune functions dependent on the presence of physiological microflora allow us to conclude that symbiotic microorganisms such as Lactobacillus sp. are essential for adequate activity of the defense system in humans. In addition to their beneficial influence on the intestinal microbial balance, these microorganisms exert a variety of immunomodulatory effects on the host immune system. On the other hand, immunostimulatory animal-derived substances rich in alkylglycerols have been shown to enhance lactic acid bacteria proliferation. Therefore, the aim of the present study was to evaluate the effects on murine humoral response of the combined administration of lyophilized combination of three lactic acid bacteria: L. acidophilus, L. bulgaricus and Bifidobacterium bifidum together with alkylglycerol-rich shark liver oil. The lactic acid bacteria mixture induced markedly stronger enhancement of the humoral response than alkylglycerols did. A significant synergistic stimulatory effect of lactic acid bacteria and alkylglycerols was observed in both treatment schedules: post- as well as in preimmunization with sheep red blood cells. However, their concomitant administration exerted stronger immunomodulatory effect than did the alternative route of treatment.
Asunto(s)
Glicerol/inmunología , Lactobacillus/inmunología , Animales , Formación de Anticuerpos , Esquema de Medicación , Sinergismo Farmacológico , Métodos de Alimentación , Inmunización , Ácido Láctico , Masculino , Ratones , Ratones Endogámicos BALB CRESUMEN
Development of new blood vessels in solid tumors depends on changes in equilibrium between angiogenic stimulators and inhibitors. Overexpression of angiogenic growth factors has been shown in bladder carcinoma. The 'mice cutaneous angiogenesis test' is a good method for assessment of the total angiogenic potential of bladder cancer tissue. The analysis of the levels of proangiogenic factors could be useful for the choice of properly directed angiogenesis inhibitors. The aim of our study was to investigate the influence of blocking some angiogenic factors on the angiogenic activity of bladder cancer tissue. Tumor tissue obtained from 12 patients with invasive bladder carcinoma was used. Cancer tissue homogenates were incubated in the presence of specific antibodies against VEGF, bFGF, Il-8 and aFGF. Cytokine levels were determined using the ELISA test. Cutaneous angiogenesis assay in Balb/c mice was performed to detect the angiogenic activity of the tumor tissue. VEGF, bFGF and Il-8 were present in all examined cancer tissues (aFGF level was not estimated). Cytokine concentration and angiogenic activity of bladder cancer tissue showed individual variation. There was no correlation between the cytokines content in tumor tissue and the ability of this tissue to induce angiogenesis. Absorption caused significant reduction in cytokines level. The reduction of angiogenic activity was observed in the cancer tissue of 1 patient after VEGF absorption, in 3 patients' tissue homogenates after incubation with anti-aFGF and in 2 patients' homogenates after bFGF absorption. There was no reduction of angiogenic activity after Il-8 absorption.
Asunto(s)
Carcinoma de Células Transicionales/irrigación sanguínea , Factores de Crecimiento Endotelial/fisiología , Factor 1 de Crecimiento de Fibroblastos/fisiología , Factor 2 de Crecimiento de Fibroblastos/fisiología , Interleucina-8/fisiología , Linfocinas/fisiología , Neovascularización Fisiológica/fisiología , Piel/irrigación sanguínea , Neoplasias de la Vejiga Urinaria/irrigación sanguínea , Animales , Anticuerpos/inmunología , Anticuerpos/farmacología , Especificidad de Anticuerpos , Carcinoma de Células Transicionales/metabolismo , Carcinoma de Células Transicionales/patología , Factores de Crecimiento Endotelial/inmunología , Factores de Crecimiento Endotelial/metabolismo , Femenino , Factor 1 de Crecimiento de Fibroblastos/inmunología , Factor 1 de Crecimiento de Fibroblastos/metabolismo , Factor 2 de Crecimiento de Fibroblastos/inmunología , Factor 2 de Crecimiento de Fibroblastos/metabolismo , Humanos , Interleucina-8/inmunología , Interleucina-8/metabolismo , Linfocinas/inmunología , Linfocinas/metabolismo , Masculino , Ratones , Ratones Endogámicos BALB C , Persona de Mediana Edad , Estadificación de Neoplasias , Neovascularización Patológica/metabolismo , Neoplasias de la Vejiga Urinaria/metabolismo , Neoplasias de la Vejiga Urinaria/patología , Factor A de Crecimiento Endotelial Vascular , Factores de Crecimiento Endotelial VascularRESUMEN
Hypoxia and sleep disorders may result in an impairment of immune system in patients with obstructive sleep apnea (OSA). Therefore we studied mitogenic stimulation of peripheral blood lymphocytes by PHA (1 microgram/ml) by measurement of 3H-thymidine incorporation, metabolic activity of neutrophils measured by chemiluminescence (CL), immunoglobulins (IgG, IgA and IgM) concentration in serum (radial immunodiffusion) and TNF-alpha concentration in serum (ELISA), and E-rosette tests (with theophylline) in 57 obese patients with severe OSA. In OSA patients severe impairment of f-MLP-induced granulocyte chemiluminescent activity was observed.
Asunto(s)
Apnea Obstructiva del Sueño/inmunología , Adulto , Anciano , Humanos , Inmunoglobulina A/sangre , Inmunoglobulina G/sangre , Inmunoglobulina M/sangre , Mediciones Luminiscentes , Activación de Linfocitos , Masculino , Persona de Mediana Edad , Neutrófilos/metabolismo , Obesidad/complicaciones , Apnea Obstructiva del Sueño/complicaciones , Factor de Necrosis Tumoral alfa/análisisRESUMEN
Preterm delivery is one of the greatest problems in obstetric care. One of the most commonly used treatments for high risk cases is salbutamol, a beta-2-adrenoceptor agonist. The aim of the present study was to determine if such treatment causes any changes in the neonatal immune system which should therefore be a concern in the care of the newborn. The experiments were performed on 4 to 5 or 6 to 7-week old female and male offspring of salbutamol-treated C3H/W inbred mice. In the first part of the study, the number of spleen cells, phenotypes and activity (phytohemagglutinin-induced proliferation, ability to induce local graft versus host reaction) were determined. We observed lowering of cell number and lowered proportions of cluster of differentiation (CD)3, CD4 and CD8 positive lymphocytes in spleens of progeny of salbutamol-treated mice. However, CD4+ to CD8+ ration was higher in the progeny of salbutamol-treated mothers than in the corresponding controls. In addition, reactivity to phytohemagglutinin and ability to induce local graft vs. host reaction were higher (popliteal lymph node test) or undisturbed (lymphocyte-induced angiogenesis test) in this group of mice.
Asunto(s)
Agonistas Adrenérgicos beta/farmacología , Albuterol/farmacología , Inmunidad Celular/efectos de los fármacos , Efectos Tardíos de la Exposición Prenatal , Bazo/efectos de los fármacos , Envejecimiento/inmunología , Animales , Animales Recién Nacidos , Femenino , Inmunofenotipificación , Activación de Linfocitos/efectos de los fármacos , Recuento de Linfocitos , Masculino , Ratones , Ratones Endogámicos C3H , Ratones Endogámicos C57BL , Embarazo , Bazo/embriología , Bazo/inmunologíaRESUMEN
Angiogenesis plays an important role in ovarian cancer growth and metastasis formation. Adenosine is one of the most potent stimulator of neovascularisation. The aim of present study was to determine if theobromine, adenosine receptor antagonist, influences angiogenic activity and proangiogenic cytokines production. Theobromine caused significant inhibition of angiogenic activity of ovarian cancer cells. In in vivo and in vitro cultures theobromine diminished vascular endothelial growth factor (VEGF) production. Production of basic fibroblast growth factor (bFGF) and interleukin-8 (IL-8) was not altered by the examined drug. These findings suggest that theobromine might be a potent inhibitor of angiogenesis induced by ovarian cancer cells and its mechanism of action is related to inhibition of VEGF production.
Asunto(s)
Adenocarcinoma Mucinoso/metabolismo , Cistadenocarcinoma Seroso/metabolismo , Citocinas/metabolismo , Neovascularización Patológica/prevención & control , Neoplasias Ováricas/metabolismo , Teobromina/farmacología , Adenocarcinoma Mucinoso/irrigación sanguínea , Adenocarcinoma Mucinoso/tratamiento farmacológico , Anciano , Anciano de 80 o más Años , Animales , Cistadenocarcinoma Seroso/irrigación sanguínea , Cistadenocarcinoma Seroso/tratamiento farmacológico , Femenino , Humanos , Ratones , Ratones Endogámicos BALB C , Persona de Mediana Edad , Trasplante de Neoplasias , Neovascularización Patológica/metabolismo , Neovascularización Patológica/patología , Neoplasias Ováricas/irrigación sanguínea , Neoplasias Ováricas/tratamiento farmacológico , Antagonistas de Receptores Purinérgicos P1 , Piel/irrigación sanguínea , Células Tumorales Cultivadas/efectos de los fármacosRESUMEN
The influence of ether fraction of water-soluble extract of poplar leaves (Populus nigra L.) and nine phenolic acids (cinnamic and benzoic acids derivatives) on human mononuclear (MNL) leukocytes angiogenic activity was studied. Ether fraction, containing mainly phenolic acids, increased cutaneous angiogenesis induced in mice by human MNL isolated from the blood of healthy donors. Increase of angiogenic activity was also observed in case of caffeic, gallic, salicylic, ferulic and gentisic acids. Isovanillic, protocatechuic and p-hydroxybenzoic acids did not influence this parameter.
Asunto(s)
Neovascularización Patológica/tratamiento farmacológico , Fenoles/farmacología , Piel/irrigación sanguínea , Árboles , Animales , Humanos , Leucocitos/efectos de los fármacos , Ratones , Ratones Endogámicos BALB C , Neovascularización Patológica/etiología , Extractos Vegetales/farmacologíaRESUMEN
Lymphocytes play a central regulatory role in mechanisms contributing to impaired function of immune system in atopy. The aim of our study was evaluate the mitogen-induced proliferation of lymphocytes in a group of asymptomatic, seasonal allergic rhinitis patients. A highly significant lower mitogen-induced proliferation and, in contrast to other studies, significantly lower background proliferative activity of lymphocytes were found in the atopic persons, comparing to the controls. We concluded that the decreased mitogen-induced proliferation of lymphocytes observed in allergic patients reflects abnormal T cell function, which is due to the atopic status, and not only as it was believed to the antigen-induced lymphocyte activation.
Asunto(s)
Activación de Linfocitos , Rinitis Alérgica Estacional/inmunología , Adolescente , Adulto , Concanavalina A/farmacología , Femenino , Humanos , Masculino , Fitohemaglutininas/farmacologíaRESUMEN
Therapeutic modalities employed in the treatment of maxillofacial malignancy, tumor resection, radiation and chemotherapy, can result in local and systemic changes in healthy oral tissues many years after anticancer therapy has been stopped. The immunologic status of a select group of nine individuals who underwent surgery of the maxillofacial region and after head and neck irradiation, with severe and persistent denture stomatitis was studied. A significant impairment of granulocyte and lymphocyte functions and increased concentrations of serum IgG, as compared to healthy controls, were found in the patients of the study group. It is concluded, that this group of patients show immunological abnormalities many years post therapy, which must be recognized by the clinician in order to effect a cure.
Asunto(s)
Candidiasis Bucal/inmunología , Neoplasias Faciales/inmunología , Granulocitos/inmunología , Huésped Inmunocomprometido , Síndromes de Inmunodeficiencia/etiología , Neoplasias Maxilomandibulares/inmunología , Infecciones Oportunistas/inmunología , Estomatitis Subprotética/inmunología , Linfocitos T/inmunología , Adulto , Anciano , Anticuerpos/sangre , Terapia Combinada , Susceptibilidad a Enfermedades/inmunología , Neoplasias Faciales/radioterapia , Neoplasias Faciales/cirugía , Femenino , Estudios de Seguimiento , Humanos , Síndromes de Inmunodeficiencia/inmunología , Neoplasias Maxilomandibulares/radioterapia , Neoplasias Maxilomandibulares/cirugía , Masculino , Persona de Mediana Edad , Complicaciones Posoperatorias/inmunología , Radioterapia/efectos adversosRESUMEN
Selected immunological parameters of peripheral blood leukocytes in 30 tb contracts and control group consisting of 30 healthy blood donors in similar age were examined. All contacts showed the exudate type Mantoux reaction (over 20 mm in diameter) and all revealed normal chest X-ray. No differences between both groups in total T cells, CD4, CD8 counts, CD4/CD8 ratio and in proliferative response to mitogens (PHA and Con A) were found. Tb contacts had the similar serum IgM and elevated IgG and IgA concentrations as compared with the controls. Tb contacts showed depressed granulocyte metabolic activity as measured by CL response to chemostatic peptide N-FORMYL-MET-LEU-PHE (FMLP) in comparison with control subjects.
Asunto(s)
Tuberculosis Pulmonar/inmunología , Adulto , Anciano , Estudios de Casos y Controles , Femenino , Granulocitos/metabolismo , Humanos , Inmunidad Celular , Inmunoglobulinas/sangre , Pulmón/diagnóstico por imagen , Activación de Linfocitos , Recuento de Linfocitos , Masculino , Persona de Mediana Edad , RadiografíaRESUMEN
The aim of the study was to compare the results of routine counts of percentage of BAL macrophages, using morphological differential staining method--Pappenheim stain (M) and staining for nonspecific esterase activity (E) with those obtained by APAAP technique, using specific for monocytes (macrophages monoclonal antibodies (Ki-M1) and the complex APAAP (alkaline phosphatase--mouse anti alkaline phosphatase complex). The mean counts were comparable, although different criteria of estimation were used. Significant differences were seen in smokers when the criteria were based on morphology and expression of surface antigens. The results indicate that correct estimation of macrophage counts and their characteristics (enzymatic activity, expression of surface antigens) can only be made using many methods based on various criteria of estimation.
Asunto(s)
Líquido del Lavado Bronquioalveolar/patología , Enfermedades Pulmonares/patología , Macrófagos/patología , Fumar/patología , Adulto , Recuento de Células Sanguíneas , Humanos , Persona de Mediana Edad , Coloración y EtiquetadoRESUMEN
The results of a preliminary analysis on the role of lymphocyte count on prognosis of therapy outcome and influencing the decision to start therapy carried out on 21 patients with sarcoidosis are presented. The decision to commence treatment was carried out after BAL was performed but the results of it were not taken in account. The patients were observed for 7 to 33 months. The course of the disease was assessed basing on clinical, radiological and functional examination. In 6 patients a control BAL was carried out. The results allow to state that high lymphocyte BAL counts do not have to be poor prognostic factors. On the other hand low counts cannot predict a benign clinical course. In the discussion the authors evaluate, basing on literature data, the role of BAL in sarcoidosis.
Asunto(s)
Líquido del Lavado Bronquioalveolar/patología , Enfermedades Pulmonares/patología , Sarcoidosis/patología , Adulto , Biomarcadores/análisis , Toma de Decisiones , Femenino , Humanos , Recuento de Leucocitos , Enfermedades Pulmonares/tratamiento farmacológico , Linfocitos/patología , Masculino , Persona de Mediana Edad , Prednisolona/uso terapéutico , Pronóstico , Sarcoidosis/tratamiento farmacológicoRESUMEN
Diagnostic usefulness of BAL was assessed in 25 selected cases of interstitial lung diseases. There were 14 cases of allergic alveolitis, 6 cases of idiopathic pulmonary fibrosis and one case each of sarcoidosis, lymphangiomyomatosis, granulomotosis bronchocentrica, lymphogranulomatosis maligna, alveolitis of unknown etiology. BAL was assessed diagnostically usefull in 77% of the cases. More helpful was medical history which proved to be useful in all cases, and pulmonary function tests which were helpful in 84%. Lung biopsy was diagnostic in 66.6% of the cases, TBLB in 36.5%, while open lung biopsy carried out in 6 patients was diagnostic in 100%. Bronchoalveolar lavage is a useful element of diagnostic evaluation in patients with interstitial lung disorders, it is a safe method and well tolerated by the patients.