RESUMEN
Transcriptional riboswitches modulate downstream gene expression by a tight coupling of ligand-dependent RNA folding kinetics with the rate of transcription. RNA folding pathways leading to functional ON and OFF regulation involve the formation of metastable states within well-defined sequence intervals during transcription. The kinetic requirements for the formation and preservation of these metastable states in the context of transcription remain unresolved. Here, we reversibly trap the previously defined regulatory relevant metastable intermediate of the Mesoplasma florum 2'-deoxyguanosine (2'dG)-sensing riboswitch using a photocaging-ligation approach, and monitor folding to its native state by real-time NMR in both presence and absence of ligand. We further determine transcription rates for two different bacterial RNA polymerases. Our results reveal that the riboswitch functions only at transcription rates typical for bacterial polymerases (10-50 nt s-1) and that gene expression is modulated by 40-50% only, while subtle differences in folding rates guide population ratios within the structural ensemble to a specific regulatory outcome.
Asunto(s)
Riboswitch/genética , Transducción de Señal/genética , Transcripción Genética , Secuencia de Bases , Simulación por Computador , Cinética , Ligandos , Espectroscopía de Resonancia Magnética , Modelos Genéticos , Conformación de Ácido NucleicoRESUMEN
In bacteria, the regulation of gene expression by cis-acting transcriptional riboswitches located in the 5'-untranslated regions of messenger RNA requires the temporal synchronization of RNA synthesis and ligand binding-dependent conformational refolding. Ligand binding to the aptamer domain of the riboswitch induces premature termination of the mRNA synthesis of ligand-associated genes due to the coupled formation of 3'-structural elements acting as terminators. To date, there has been no high resolution structural description of the concerted process of synthesis and ligand-induced restructuring of the regulatory RNA element. Here, we show that for the guanine-sensing xpt-pbuX riboswitch from Bacillus subtilis, the conformation of the full-length transcripts is static: it exclusively populates the functional off-state but cannot switch to the on-state, regardless of the presence or absence of ligand. We show that only the combined matching of transcription rates and ligand binding enables transcription intermediates to undergo ligand-dependent conformational refolding.
Asunto(s)
Bacillus subtilis/genética , Regulación Bacteriana de la Expresión Génica , Pliegue del ARN , ARN Bacteriano/química , ARN Bacteriano/metabolismo , Riboswitch , Transcripción Genética , Espectroscopía de Resonancia Magnética , Modelos Biológicos , Modelos Moleculares , Conformación de Ácido NucleicoRESUMEN
Determination of RNA secondary structures by NMR spectroscopy is a useful tool e.g. to elucidate RNA folding space or functional aspects of regulatory RNA elements. However, current approaches of RNA synthesis and preparation are usually time-consuming and do not provide analysis with single nucleotide precision when applied for a large number of different RNA sequences. Here, we significantly improve the yield and 3' end homogeneity of RNA preparation by in vitro transcription. Further, by establishing a native purification procedure with increased throughput, we provide a shortcut to study several RNA constructs simultaneously. We show that this approach yields µmol quantities of RNA with purities comparable to PAGE purification, while avoiding denaturation of the RNA.
Asunto(s)
Resonancia Magnética Nuclear Biomolecular , Conformación de Ácido Nucleico , ARN/química , ARN/metabolismo , Aptámeros de Nucleótidos/química , Secuencia de Bases , Tampones (Química) , Ligandos , Datos de Secuencia Molecular , ARN/aislamiento & purificación , Riboswitch , Transcripción GenéticaRESUMEN
Unlike proteins, a given RNA sequence can adopt more than a single conformation. The two (or more) conformations are long-lived and have similar stabilities, but interconvert only slowly. Such bi- or multistability is often linked to the biological functions of the RNA. This unit describes how nuclear magnetic resonance (NMR) spectroscopy can be used to characterize the conformational dynamics of bistable RNAs.