RESUMEN
Expression of class II molecules by epidermal cells of BALB/c nude mice following an adoptive transfer of lymphoid cells from normal semisyngeneic mice has been previously observed. The purpose of the present study was to determine whether similar expression might be observed after injection of human lymphocytes and to utilize this model to determine whether there would be expression of class II molecules by mucosal and epidermal cells following administration of lymphocytes from young and old donors. In order to address this question, a group of nude mice received a single i.v. injection of human T lymphocytes obtained from aged subjects, and a second group received i.v. injection of lymphocytes from young volunteers. Evaluation of I-A and I-E molecules expression of murine cells were performed by indirect immunoperoxidase staining. More prominent and diffuse staining of class II was observed in the mucosal cells as compared with the keratinocytes. Diffuse and intense staining of intestinal cells was observed mainly in mice treated with lymphocytes from young donors.
Asunto(s)
Antígenos de Histocompatibilidad Clase II/análisis , Mucosa Intestinal/inmunología , Intestino Delgado/inmunología , Queratinocitos/inmunología , Ratones Desnudos/inmunología , Linfocitos T/inmunología , Traslado Adoptivo , Adulto , Anciano , Anciano de 80 o más Años , Animales , Senescencia Celular/inmunología , Humanos , Mucosa Intestinal/citología , Intestino Delgado/citología , Ratones , Ratones Endogámicos BALB C , Persona de Mediana Edad , Organismos Libres de Patógenos EspecíficosRESUMEN
The changes in serum and hepatic lipid fractions (LT, Col, TG, beta-LP), aggregability indices (IAg), platelet adhesiveness (IA), atherogenicity (Ia) and microscopic aspect of aorta and myocardium in three groups of animals injected with vitamin D2: normal, orchiectomized and treated with testosterone, were followed up. In the normal animals the injection of vitamin D2 induced the increase of all lipid fractions, of IA, IAg, Ia and histologically microcalcifications at the coronaries level were present; in the orchiectomized animals the biochemical changes were less marked and histologically thickenings of aortic and coronary endothelium with areas of intestinal sclerosis in the myocardium were found. In the animals with hyperandrogenism, the administration of vitamin D2 induced the decrease of TG and increase of IAg and IA; histologically, aspects similar to those noticed after the administration of testosterone alone were present. It is concluded that hypervitaminosis D induces a marked increase of serum lipid fractions in the normal animals, while the histological examination revealed more marked changes in conditions of sexual function cessation.