RESUMEN
The study was performed on 21 H-F calves divided into 3 groups of 7 animals each. The first group was composed of calves whose mothers did not receive an injection of Se preparation. The second and third groups consisted of calves whose mothers were administered injections of Se and vitamin E in a single dose of 10 ml and 30 ml, 10 days before the expected parturition date. 24 hours after birth, blood samples were collected from all calves to determine Se, Fe and IgG concentrations and the activity of GSH-Px and GGT. The results of the study indicate that the administration of a single-dose Se supplement to cows in late pregnancy increases Se concentration in calves and promotes passive transfer of immunity from the mother to offspring.
Asunto(s)
Suplementos Dietéticos , Hierro , Selenio , Animales , Bovinos , Femenino , Hierro/sangre , Embarazo , Selenio/farmacocinética , Selenio/fisiología , Vitamina E/farmacocinéticaRESUMEN
Cytological evaluation of bone marrow smears stained by May-Grünwald Giemsa method was performed. The smears came from 20 fallow deer (Dama dama) 3 days old divided into 2 groups each consisting of 10 animals. The experimental group (E) received intramuscularly selenium and vitamin E at a dose of 3.0 ml (tocopherol acetate - 50 mg, sodium selenite - 0.5 mg, solvent - 1 ml) in the 3rd day of age. The control group (C) did not receive any supplementation or placebo. For hematological analyzes blood was collected three times: on 0, 15th and 25th day of the experiment. Serum concentration of selenium and vitamin E was determined using high perfor- mance liquid chromatography and glutathione peroxidase activity (GSH-Px) by kinetic method. On the 15th day after supplementation, a statistically significant increase in the percentage of erythroblastic cell line was observed in bone marrow smears. At that time, the increase in GSH-Px activity in the E group was also observed, reaching the value of 165.3 U/gHb, which was statisti- cally significant. The percentage of proerythroblasts (8.23% in group E and 5.02% in group C) differed significantly between groups at the 25th day after supplementation. This study revealed that supplementation of selenium and vitamin E resulted in an increase in the number of erythro- cytes to an average of 13.5 (Ë 10¹²/l) in the experimental group on 25th day with a significant increase in hemoglobin to 193 g/l in the experimental group.
Asunto(s)
Células de la Médula Ósea/fisiología , Ciervos/sangre , Eritrocitos/efectos de los fármacos , Selenio/farmacología , Vitamina E/farmacología , Alimentación Animal/análisis , Animales , Dieta/veterinaria , Suplementos Dietéticos , Eritrocitos/citología , Selenio/administración & dosificación , Vitamina E/administración & dosificaciónRESUMEN
Simvastatin is a drug commonly used to reduce the cholesterol level. 32 clinically healthy female pigs with a bodyweight of 35-40 kg, kept in standard laboratory conditions were chosen for the experiment and divided into 2 groups (control and experimental) consisting of 16 animals. The experimental group received simvastatin orally at a dose of 40 mg (one tablet once a day) for 56 days, and at the same time the control group received placebo (empty gelatin capsules). Bone marrow smears and peripheral blood samples were evaluated. The obtained results indicate that simvastatin may inhibit erythropoiesis even after a relatively short period of administration, and observed changes can be the cause of some symptoms (for example anemia) occurring during statin therapy.
Asunto(s)
Células de la Médula Ósea/efectos de los fármacos , Linaje de la Célula/efectos de los fármacos , Eritrocitos/efectos de los fármacos , Simvastatina/farmacología , Porcinos , Animales , Anticolesterolemiantes/farmacología , Eritrocitos/fisiología , FemeninoRESUMEN
The aim of the study was to evaluate the megakaryocyte lineage of bone marrow and coagulation parameters in fallow deer during the last month of pregnancy. The animals were managed in the barn-feeding system. Twenty female fallow deer, aged 2-3 years, divided into 2 groups were used in the study. Group 1 comprised the females in the last month of pregnancy, and the non-pregnant females were used as the control. All the animals were clinically healthy. Coagulation parameters were measured in all the deer: thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time (APTT), and plasma concentrations of fibrinogen, D-dimer, and antithrombin III. A quantitative assessment of bone marrow was carried out for the erythroblastic, myeloid, lymphoid, monocyte-macrophage, and megakaryopoietic cell lines. A detailed analysis of megakaryocyte lineage was performed after whole blood and platelet count. There were no significant differences in the erythroblast, granulocyte, monocyte-macrophage and lymphoid systems between the animal groups. Thrombocyte count in the pregnant deer was lower than that found in the control group. Bone marrow smears revealed a slightly decreased megakaryocyte count, while the megakaryoblast and promegakaryocyte counts were unchanged. The analysis of coagulation parameters showed increased levels of fibrinogen, thrombin time, prothrombin time and activated partial thromboplastin time in the pregnant animals. The study suggested a hyperactivation of the coagulation system with a slight reduction in the megakaryocyte count in bone marrow, and a reduction in platelet count in peripheral blood at the end of pregnancy.
Asunto(s)
Coagulación Sanguínea/fisiología , Células de la Médula Ósea/fisiología , Ciervos/fisiología , Megacariocitos/fisiología , Preñez , Animales , Femenino , EmbarazoRESUMEN
The aim of this study was to determine the presence of cocaine- and amphetamine-regulated transcript-like immunoreactive (CART-LI) neurons and co-localisation of CART with vesicular acetylcholine transporter (VAChT), neuronal nitric oxide synthase (n-NOS), vasoactive intestinal polypeptide (VIP), substance P (SP) and leu-enkephalin (LENK) in the enteric nervous system of the porcine gastric cardia by using a double-labelling immunofluorescence technique. CART-LI neurons were observed in the myenteric plexus (18.2±2.6%). A dense network of CART-LI nerve fibers was mainly observed in the muscular layer. CART showed co-localization mainly with VAChT, n-NOS, VIP and to a lesser degree with LENK and SP. Distribution of CART and its co-localization with other neurotransmitters suggest that this peptide plays an important role in gastric motility in the pig.
Asunto(s)
Cardias/inervación , Proteínas del Tejido Nervioso/metabolismo , Neuronas/metabolismo , Porcinos/fisiología , Animales , Regulación de la Expresión Génica/fisiología , Proteínas del Tejido Nervioso/genética , Neuronas/clasificaciónRESUMEN
The purpose of this study was to investigate possible alterations in acid-base balance parameters and the coagulation profile in neonatal diarrheic calves. Twenty neonatal diarrheic and 20 clinically healthy neonatal calves aged between 1 week to 10 days were used. All blood samples were taken on the third day from the onset of diarrhea symptom. Venous blood samples were collected from each animal to determine platelet numbers, pH, pCO2, pO2, HCO(3-), BE, O2SAT, ctCO2 and electrolytes (K+, Na+ and Cl(-)). Plasma samples were collected from each animal for the measurement of prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), the concentrations of fibrinogen, D-dimer and the activity of antithrombin III (AT III). Blood pH (7.19), BE (-10.6 mmol/l), HCO3(-) (25.15 mmol/l), pO2 (3.33 kPa), O2SAT (24.12 %) were significantly lower and serum concentration of K+ (6.55 mmol/l) was significantly higher in diarrheic calves. These changes indicate the state of uncompensated metabolic acidosis with accompanying hyperkalemia. TT (32.05s) and APTT (39.9s) values were more prolonged in calves with diarrhea than in the control group. D-dimer (587.25 microg/l) concentrations were significantly increased while a visible drop in AT III (103.75%) activity and platelets counts (598 x10(9)/l) were observed in diarrheic group of calves. The results suggest that a consumptive type of disseminated intravascular coagulation (DIC) developed in diarrheic calves.
Asunto(s)
Equilibrio Ácido-Base/fisiología , Coagulación Sanguínea/fisiología , Enfermedades de los Bovinos/sangre , Diarrea/veterinaria , Animales , Estudios de Casos y Controles , Bovinos , Diarrea/sangre , Diarrea/metabolismo , Humanos , Recién NacidoRESUMEN
The purpose of this study was to determine changes in coagulation profile parameters in cattle with left abomasal displacement (LAD). The study was performed on 20 Holstein-Friesian (H-F) cows divided into two groups: group I--10 cows with diagnosed left abomasal displacement and group II--10 clinically healthy cows. Coagulation tests, including TT (thrombin time), PT (prothrombin time) and APTT (activated partial thromboplastin time), were conducted, and fibrinogen content, D-dimer content, AT III (antithrombin III) activity and platelet (PLT) count were determined in all the animals. Prolonged TT, PT and APTT, a higher fibrinogen and D-dimer content, a drop in AT III activity and thrombocyte count were observed in the cattle with LAD. The above abnormal coagulation profiles were most predominant in three cows which died after surgical repositioning of the abomasum. The results of the study indicate that in cattle with abomasal displacement, the disseminated intravascular coagulation (DIC) syndrome was the most significant risk factor for mortality.