RESUMEN

The complete amino acid sequence of a mammalian acetylcholinesterase from fetal bovine serum (FBS AChE) is presented. This enzyme has a high degree of sequence identity with other cholinesterases, liver carboxyesterases, esterase-6, lysophospholipase, and thyroglobulin. The locations of 191 amino acids in 10 regions of the FBS enzyme were compared with corresponding sequences of Torpedo, human, and Drosophila AChEs and human serum butyrylcholinesterase (BChE). In one region there is a marked difference in both the number of amino acids and their sequence between mammalian AChE and other AChEs and the human serum BChE. The amino acid sequence of FBS AChE showed overall homologies of 90% with human AChE, 60% with T. california AChE, 50% with human serum BChE, and 39% with Drosophila AChE in these regions.

Asunto(s)

Acetilcolinesterasa/sangre , Acetilcolinesterasa/genética , Secuencia de Aminoácidos , Animales , Bovinos , Colinesterasas/genética , Datos de Secuencia Molecular

Asunto(s)

Acetilcolinesterasa/sangre , Acetilcolinesterasa/inmunología , Secuencia de Aminoácidos , Animales , Anticuerpos Monoclonales , Catálisis , Bovinos , Reacciones Cruzadas , Epítopos/análisis , Sangre Fetal/enzimología , Humanos , Datos de Secuencia Molecular , Mapeo Peptídico , Conformación Proteica , Especificidad de la Especie , Torpedo